I control microplate reader software v1

Accutase was added to each well and plates were incubated at 37°C for 10 minutes to detach cells. Cells were dislodged, placed in tubes, and centrifuged for 5 min at 400 x g at RT. The pellet was resuspended and a SensoLyte ® 520 TACE (α - Secretase) Activity Assay Kit was used to assess TACE activity (per instructions). After adding the TACE substrate solution into each well, the plate was mixed and sample fluorescence intensity was immediately recorded by an Infinite M1000 PRO microplate reader (Tecan) using i-controlTM microplate reader software v1.6.19.2 (Tecan) or Synergy H1 microplate reader (BioTek Instruments, Inc., Winooski, Vermont, USA) using Gen5 software (BioTek Instruments, Inc.). Excitation/emission wavelengths were 490/520 nm. An aliquot of live cell suspension was used for manual cell counting on a hemocytometer to determine cell density. Raw TACE activity values were normalized to the cell density and to untreated controls that originated from the same colon.