The epididymal adipose tissue and liver and spleen fresh masses were determined gravimetrically. Mice carcass composition was determined by weighing carcass before and after water drying and before and after lipid extraction of the dried carcass with petroleum ether as previously described by Salerno et al. [16 (link)]. Liver lipids were extracted using the Folch method [17 (link)]. The liver content of cholesterol and triglycerides was determined using colorimetric-enzymatic assays (Roche-Hitachi, Germany) after dissolving the lipid extracts in a triton-containing buffer (0.1 M potassium phosphate, pH 7.4, 0.05 M NaCl, 5 mM cholic acid, and 0.1% Triton X-100).
Colorimetric enzymatic assays
Manufactured by Roche
Sourced in Italy
Colorimetric enzymatic-assays are laboratory instruments designed to measure the concentration of specific analytes in a sample through a color-based reaction. The core function of these assays is to quantify the presence and amount of a target substance by analyzing the intensity of the color change that occurs during a enzymatic reaction.
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Lab products found in correlation
8 protocols using colorimetric enzymatic assays
1
Carcass Composition and Lipid Analysis
2
Serum Lipid Profile Measurement
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Serum glucose level was measured by the glucose oxidase enzymatic method (Roche Diagnostics, Monza, Italy). The sensitivity of the method was 2 mg/dL.
Colorimetric enzymatic-assays (Roche Diagnostics, Monza, Italy) were used to determine serum T-C and HDL-C levels. The sensitivities of the assays were 3.86 mg/dL and 3.09 mg/dL, respectively.
Colorimetric enzymatic-assays (Roche Diagnostics, Monza, Italy) were used to determine serum T-C and HDL-C levels. The sensitivities of the assays were 3.86 mg/dL and 3.09 mg/dL, respectively.
3
Metabolic Biomarker Quantification Protocol
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Blood samples (about 10 mL) were collected at around 8:00 AM after an overnight fast. Aspartate aminotransferase (AST), T-C, HDL-C, LDL-C, TG, total bilirubin, uric acid, glucose, insulin and CRP were measured. Colorimetric enzymatic-assays (Roche Diagnostics, Monza, Italy) were used to determine serum AST, T-C, LDL-C, HDL-C, TG, uric acid and total bilirubin levels. The sensitivities of the method for each parameter were 5 U/L, 3.86 mg/dL, 3.87 mg/dL, 3.09 mg/dL, 8.85 mg/dL, 0.2 mg/dL and 0.146 mg/dL, respectively.
The serum glucose level was measured by the glucose oxidase enzymatic method (Roche Diagnostics, Monza, Italy). The sensitivity of the method was 2 mg/dL. The serum insulin concentration was determined by a chemiluminescent immunometric assay, using a commercial kit (Elecsys Insulin, Roche Diagnostics, Monza, Italy). The sensitivity of the method was 0.2 µIU/mL.
Insulin resistance was estimated using the HOMA-IR method [39 (link)]. CRP was measured using an immunoturbidimetric assay (CRP RX, Roche Diagnostics GmbH, Mannheim, Germany). The sensitivity of the method was 0.03 mg/dL. APRI was calculated by means of the following formula: (AST [IU/L]/40)/platelet count [109/L] * 100.
The serum glucose level was measured by the glucose oxidase enzymatic method (Roche Diagnostics, Monza, Italy). The sensitivity of the method was 2 mg/dL. The serum insulin concentration was determined by a chemiluminescent immunometric assay, using a commercial kit (Elecsys Insulin, Roche Diagnostics, Monza, Italy). The sensitivity of the method was 0.2 µIU/mL.
Insulin resistance was estimated using the HOMA-IR method [39 (link)]. CRP was measured using an immunoturbidimetric assay (CRP RX, Roche Diagnostics GmbH, Mannheim, Germany). The sensitivity of the method was 0.03 mg/dL. APRI was calculated by means of the following formula: (AST [IU/L]/40)/platelet count [109/L] * 100.
4
Fasting Lipid and Glucose Assessment
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Blood samples (a total of about 20 mL) were collected at around 8:00 AM after an overnight fast only at the beginning of the BWRP. Lipids (high-density lipoprotein (HDL) cholesterol and triglycerides) and glucose were measured.
Serum glucose level was measured by the glucose oxidase enzymatic method (Roche Diagnostics, Monza, Italy). The sensitivity of the method was 2 mg/dL [1 mg/dL = 0.06 mmol/L].
Colorimetric enzymatic-assays (Roche Diagnostics, Monza, Italy) were used to determine serum HDL cholesterol and triglycerides levels. The sensitivities of the assays were 3.09 mg/dL [1 mg/dL = 0.03 mmol/L] and 8.85 mg/dL [1 mg/dL = 0.01 mmol/L], respectively.
Serum glucose level was measured by the glucose oxidase enzymatic method (Roche Diagnostics, Monza, Italy). The sensitivity of the method was 2 mg/dL [1 mg/dL = 0.06 mmol/L].
Colorimetric enzymatic-assays (Roche Diagnostics, Monza, Italy) were used to determine serum HDL cholesterol and triglycerides levels. The sensitivities of the assays were 3.09 mg/dL [1 mg/dL = 0.03 mmol/L] and 8.85 mg/dL [1 mg/dL = 0.01 mmol/L], respectively.
5
Metabolic Profiling of Fasted Participants
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Approximately 10 mL of blood samples were obtained from each participant in standard tubes at 8:00 AM following an overnight fast. Subsequently, blood count and metabolic variables were assessed.
Hematologic parameters were analyzed using Beckman Coulter instruments. Leukocyte count was performed with the impedance-based method upon erythrocyte (RBC) lysis. The determination of leukocyte subtype populations relied on the assessment of volume, conductivity, and scatter properties of leukocytes (VCS Technology).
Colorimetric enzymatic assays (Roche Diagnostics, Monza, Italy) were used to determine serum HDL-C and triglyceride levels. Serum glucose level was measured by the glucose oxidase enzymatic method (Roche Diagnostics, Monza, Italy). All serum analyses for the determination of HDL-C, triglycerides, and glucose were performed by using a Roche Cobas 6000 analyzer.
Hematologic parameters were analyzed using Beckman Coulter instruments. Leukocyte count was performed with the impedance-based method upon erythrocyte (RBC) lysis. The determination of leukocyte subtype populations relied on the assessment of volume, conductivity, and scatter properties of leukocytes (VCS Technology).
Colorimetric enzymatic assays (Roche Diagnostics, Monza, Italy) were used to determine serum HDL-C and triglyceride levels. Serum glucose level was measured by the glucose oxidase enzymatic method (Roche Diagnostics, Monza, Italy). All serum analyses for the determination of HDL-C, triglycerides, and glucose were performed by using a Roche Cobas 6000 analyzer.
6
Blood Collection and Metabolic Analysis
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About 10 mL of blood samples were collected in standard tubes at 8:00 AM after an overnight fast. Blood count and metabolic variables were than determined.
Hematologic parameters were measured using Beckman Coulter instruments. Leukocytes count was performed with the impedance-based method upon erythrocyte (RBC) lysis. Volume, conductivity and scatter properties of leukocytes (VCS Technology) were used to determine leukocytes populations.
Colorimetric enzymatic assays (Roche Diagnostics, Monza, Italy) were used to determine serum HDL-C and triglycerides levels. Serum glucose level was measured by the glucose oxidase enzymatic method (Roche Diagnostics, Monza, Italy). All serum analysis on HDL-C, triglycerides and glucose were performed by using the Roche Cobas 6000 analyzer.
Hematologic parameters were measured using Beckman Coulter instruments. Leukocytes count was performed with the impedance-based method upon erythrocyte (RBC) lysis. Volume, conductivity and scatter properties of leukocytes (VCS Technology) were used to determine leukocytes populations.
Colorimetric enzymatic assays (Roche Diagnostics, Monza, Italy) were used to determine serum HDL-C and triglycerides levels. Serum glucose level was measured by the glucose oxidase enzymatic method (Roche Diagnostics, Monza, Italy). All serum analysis on HDL-C, triglycerides and glucose were performed by using the Roche Cobas 6000 analyzer.
7
Fasting Blood Lipid and Glucose Profiles
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Blood samples (about 10 mL) were collected at around 8:00 a.m. after an overnight fast at the beginning of the BWRP at T1 and T2. Total cholesterol (T-C), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), triglycerides (TG), glucose and insulin were measured.
Colorimetric enzymatic-assays (Roche Diagnostics, Monza, Italy) were used to determine serum T-C, LDL-C, HDL-C and TG levels. The sensitivities of the assays were 3.86 mg/dL [1 mg/dL = 0.03 mmol/L], 3.87 mg/dL [1 mg/dL = 0.03 mmol/L], 3.09 mg/dL [1 mg/dL = 0.03 mmol/L] and 8.85 mg/dL [1 mg/dL = 0.01 mmol/L], respectively.
Serum glucose level was measured by the glucose oxidase enzymatic method (Roche Diagnostics, Monza, Italy). The sensitivity of the method was 2 mg/dL [1 mg/dL = 0.06 mmol/L].
Serum insulin concentration was determined by a chemiluminescent immunometric assay, using a commercial kit (Elecsys Insulin, Roche Diagnostics, Monza, Italy). The sensitivity of the method was 0.2 µIU/mL [1 µU/mL = 7.18 pmol/L].
The intra- and inter-assay coefficients of variation (CVs) were the following: 1.1% and 1.6% for T-C, 1.2% and 2.5% for LDL-C, 1.8% and 2.2% for HDL-C, 1.1% and 2.0% for TG, 1.0% and 1.3% for glucose, and 1.5% and 4.9% for insulin.
Colorimetric enzymatic-assays (Roche Diagnostics, Monza, Italy) were used to determine serum T-C, LDL-C, HDL-C and TG levels. The sensitivities of the assays were 3.86 mg/dL [1 mg/dL = 0.03 mmol/L], 3.87 mg/dL [1 mg/dL = 0.03 mmol/L], 3.09 mg/dL [1 mg/dL = 0.03 mmol/L] and 8.85 mg/dL [1 mg/dL = 0.01 mmol/L], respectively.
Serum glucose level was measured by the glucose oxidase enzymatic method (Roche Diagnostics, Monza, Italy). The sensitivity of the method was 2 mg/dL [1 mg/dL = 0.06 mmol/L].
Serum insulin concentration was determined by a chemiluminescent immunometric assay, using a commercial kit (Elecsys Insulin, Roche Diagnostics, Monza, Italy). The sensitivity of the method was 0.2 µIU/mL [1 µU/mL = 7.18 pmol/L].
The intra- and inter-assay coefficients of variation (CVs) were the following: 1.1% and 1.6% for T-C, 1.2% and 2.5% for LDL-C, 1.8% and 2.2% for HDL-C, 1.1% and 2.0% for TG, 1.0% and 1.3% for glucose, and 1.5% and 4.9% for insulin.
8
Fasting Biomarker Measurement Protocol
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Blood samples (about 10 mL) were collected at around 8 : 00 AM after an overnight fast at the beginning of the BWRP. High-density lipoprotein cholesterol (HDL-C), triglycerides, and glucose were measured.
Colorimetric enzymatic assays (Roche Diagnostics, Monza, Italy) were used to determine serum HDL-C and triglycerides levels. The sensitivities of the assays were 3.09 mg/dL (1 mg/dL = 0.03 mmol/L) and 8.85 mg/dL (1 mg/dL = 0.01 mmol/L), respectively.
Serum glucose level was measured by the glucose oxidase enzymatic method (Roche Diagnostics, Monza, Italy). The sensitivity of the method was 2 mg/dL (1 mg/dL = 0.06 mmol/L).
Colorimetric enzymatic assays (Roche Diagnostics, Monza, Italy) were used to determine serum HDL-C and triglycerides levels. The sensitivities of the assays were 3.09 mg/dL (1 mg/dL = 0.03 mmol/L) and 8.85 mg/dL (1 mg/dL = 0.01 mmol/L), respectively.
Serum glucose level was measured by the glucose oxidase enzymatic method (Roche Diagnostics, Monza, Italy). The sensitivity of the method was 2 mg/dL (1 mg/dL = 0.06 mmol/L).
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