Hze j

Manufactured by Jackson ImmunoResearch

The Hze/J is a lab equipment product offered by Jackson ImmunoResearch. It serves as a core function for laboratory applications, but a detailed description while maintaining an unbiased and factual approach is not available.

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6 protocols using hze j

Fate Mapping of Regulatory T Cells

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For fate mapping of exTregs, Foxp3^{eGFP-Cre-ERT2} (Jackson; #016961) mice were crossed to B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J (Jackson; #007914) and B6.129P2-Apoe^tm1Unc/J (Jackson; #002052) to obtain the lineage tracker Apoe^−/− mice (Foxp3^{eGFP-Cre-ERT2 (link)};Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J;129P2-Apoe^tm1Unc/J, ROSA26-SORT-CAG/FoxP3-eGFP-ERT-Cre/Apoe^−/−). Mice received tamoxifen injections (75 mg/kg body weight; i.p) for 5 days at 8 weeks old and again one week before the experiment to induce Cre, resulting in GFP and RFP expression in Foxp3⁺ cells. These animals were used to distinguish current Tregs (GFP+RFP+) from exTregs (GFP-RFP+). 8-10 weeks old female B6.129P2-Apoe^tm1Unc/J mice (Apoe^−/−) were used as recipients for adoptive transfer experiments. The housing conditions for these mice were as follows; lights on at 6am and off at 6pm, ambient temperature between 69-75 degrees, humidity within 30%-70% (average 40%, outdoor environment can affect humidity inside).

Saigusa R., Roy P., Freuchet A., Gulati R., Ghosheh Y., Suthahar S.S., Durant C.P., Hanna D.B., Kiosses W.B., Orecchioni M., Wen L., Wu R., Kuniholm M.H., Landay A.L., Anastos K., Tien P.C., Gange S.J., Kassaye S., Vallejo J., Hedrick C.C., Kwok W.W., Sette A., Hodis H.N., Kaplan R.C, & Ley K. (2022). Single cell transcriptomics and TCR reconstruction reveal CD4 T cell response to MHC-II-restricted APOB epitope in human cardiovascular disease. Nature cardiovascular research, 1(5), 462-475.

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Transgenic Mouse Lines for Lineage Tracing

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B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J (007914), B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J (007676), B6D2F1/J (100006), B6.Cg-Pdgfrbtm1.1(cre/ERT2)Csln/J (030201), B6.Cg-Tg(Tek-cre)1Ywa/J (008863), B6.129P2(C)-Cx3cr1tm2.1(cre/ERT2)Jung/J (020940), and B6.129S4-Gt(ROSA)26Sortm2(FLP*)Sor/J (012930) were obtained from Jackson Laboratories. Hoxa13^Cre-WPRE and Hoxa13^Cre mice generated in this study were described below. For timed matings, embryonic day 0.5 (E0.5) was identified by the time a vaginal plug was discovered. The pregnant dams at the indicated times were sacrificed and the embryo and placenta were dissected out from the uterus. All experiments were performed at least twice using different litters and used littermate controls on a mixed background unless otherwise indicated. These mice were maintained in a specific pathogen-free environment under a 14/10 hour light/dark cycle and all animal experiments described were performed in accordance and approval of the University of Pennsylvania Institutional Animal Care and Use Committee.

Chen X., Tang A.T., Tober J., Yang J., Leu N.A., Sterling S., Chen M., Yang Y., Mericko-Ishizuka P., Speck N.A, & Kahn M.L. (2022). Mouse placenta fetal macrophages arise from endothelial cells outside the placenta. Developmental cell, 57(23), 2652-2660.e3.

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Mouse Breeding and Genotyping Protocol

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Mice were housed and bred under specific pathogen–free conditions at the UCSF Laboratory Animal Research Center. Male and female mice between 8 and 12 weeks of age were used for the experiments. WT C57BL/6J, C57BL/6-Tg(Pf4-icre)Q3Rsko/J, B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTdTomato)Hze}/J, B6.129(Cg)-Gt(ROSA)26Sor^{tm4(ACTB-tdTdTomato,-EGFP)Luo}/J, C57BL/6-Tg(UBC-GFP)30Scha/J, B6.129(Cg)-Lepr^tm2(cre)Rck/J and B6.129S2-CD40lg^tm1lmx/J mice were purchased from The Jackson Laboratory. c-mpl^−/− mice (on a C57BL/6 background) were obtained from Genentech through a material transfer agreement.

Valet C., Magnen M., Qiu L., Cleary S.J., Wang K.M., Ranucci S., Grockowiak E., Boudra R., Conrad C., Seo Y., Calabrese D.R., Greenland J.R., Leavitt A.D., Passegué E., Méndez-Ferrer S., Swirski F.K, & Looney M.R. (2022). Sepsis promotes splenic production of a protective platelet pool with high CD40 ligand expression. The Journal of Clinical Investigation, 132(7), e153920.

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Generating Transgenic Gli1-CE;tdT Mice

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All animal procedures were reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) at Stanford University. The following mice were purchased from The Jackson Laboratory (Sacramento, CA): FVB (Stock Number # 001800), Gli1^{tm3(cre/ERT2)Alj/J}(Gli1-CreER^T2 (Stock number # 007913)) and B6.Cg-Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}/J(Stock number # 007909). To obtain Gli1^{CreERT2;tdTomato} (denoted as Gli1-CE;tdT) mice, heterozygous Gli1-^CreERT2 mice were crossed with homozygous B6.Cg-Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze/J} mice. To confirm mouse genotypes, tail samples of pups under 21 days of age were analyzed by PCR. Tail samples were digested using Quick Extract DNA Extraction Solution (Fisher, cat # QE09050) and PCR was performed on DNA samples using a 2700 ABI thermocycler (Applied Biosystems). The program of the thermocycler was as follows: 95 – 5 minutes, 35 cycles of :95 – 45 seconds, 55 – 45 seconds, 72 – 45 seconds, then 72 – 10 minutes and 10 – hold. List of Primers are shown in Table 1.

Faniku C., Kong W., He L., Zhang M., Lilly G, & Pepper J. (2020). Hedgehog signaling promotes endoneurial fibroblast migration and Vegf-A expression following facial nerve injury. Brain research, 1751, 147204.

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Diabetic Fracture Healing in Mice

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All animal experiments were initiated on 8- to 10-week-old male and female mice conforming to a protocol approved by the University of Pennsylvania Institutional Animal Care and Use Committee. The following mice were purchased from The Jackson Laboratory: C57BL/6J, B6.Cg-Tg(Prrx1-cre)1Cjt/J (Prx1^Cre), B6.Cg-Tg(Prrx1-cre/ERT2,-EGFP)1Smkm/J (Prx1^CreER), B6.Cg-Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}/J (R26^TdTomato), and B6.129P2-Gt(ROSA)26Sor^tm1(DTA)Lky/J (R26^DTA). B6;SJL-Tg(Col2α1-cre)1Bhr/J mice (Col2α1^Cre) were obtained from Dr. Patrick O’Connor (Rutgers University, Newark, NJ), and floxed Ikkβ mice (Ikkβ^f/f) from Dr. Michael Karin (University of California San Diego, La Jolla, CA). Low-dose streptozotocin injection was performed intraperitoneally for T1D induction (5 consecutive days, 50 mg/kg) (Cayman Chemical) as previously described (22 (link)). Animals were considered diabetic when blood glucose was >220 mg/dL 1 week after last injection and remained diabetic for 4–6 weeks prior to fracture surgery.

Ko K.I., Syverson A.L., Kralik R.M., Choi J., DerGarabedian B.P., Chen C, & Graves D.T. (2019). Diabetes-Induced NF-κB Dysregulation in Skeletal Stem Cells Prevents Resolution of Inflammation. Diabetes, 68(11), 2095-2106.

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Tracing Tbet+ Cell Lineage

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C57BL/6J female mice aged 6-8 weeks old were purchased from Jackson Laboratories or bred in-house at La Jolla Institute for Immunology. B6;CBA-Tg(Tbx21-cre)1Dlc/J (Tbet-cre) were purchased from Jackson Laboratories and then bred with B6.Cg-Gt(ROSA)26Sor tm14(CAG-tdTomato)Hze/J (Td-tomato) mice (also obtained from approved by the Institutional Animal Care and Use Committee at the La Jolla Institute for Immunology.

Murray M., Engel I., Seumois G., Mata S.H.l., Rosales S.L., Sethi A., Premlal A.L.R., Seo G., Greenbaum J., Vijayanand P., Scott‐Browne J., & Kronenberg M. (2020). Transcriptome and Chromatin Landscape of iNKT cells are Shaped by Subset Differentiation and Antigen Exposure.

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