To observe the stomatal morphology, we used a published protocol [30 (link)]. To remove any debris, leaves were acetylated in 80% ethanol for two to three min. The tiny sections of leaf were prepared using s-cutting, and after that platinum was used to fix the abaxial and adaxial surfaces and sputtered using Leica Mikrosystem GmbH (ACE600) for 25 min, and finally examined under a SEM (Thermo Scientific, Verios G4 UC, Waltham, MA, USA).
Ace600
Manufactured by Thermo Fisher Scientific
Sourced in United States
The ACE600 is a compact and versatile laboratory centrifuge designed for a wide range of applications. It features a high-capacity rotor that can accommodate multiple sample sizes, allowing for efficient and reliable sample separation. The ACE600 is built with a durable and user-friendly interface, making it a reliable and efficient tool for laboratory professionals.
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Lab products found in correlation
2 protocols using ace600
1
Stomatal Morphology Visualization Protocol
2
Leaf Surface Imaging by SEM
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For this purpose, a previously published protocol with slight modification was followed [82 (link)]. The dried leaves were first acetylated for 2 to 3 min in 80% ethanol to eliminate any debris. The s-cutting was used to prepare tiny sections of leaf, then both abaxial and adaxial surfaces were fixed on the stub, sputtered with platinum for 25 min using Leica Mikrosystem GmbH, (ACE600), and finally studied with a scanning electron microscope (Thermo Scientific, Model: verios G4 UC) present in Hainan University, Haikou China.
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