The expression of RXFP1 and GR (liver, heart, kidney, lung, esophagus) was examined using rabbit anti-RXFP1 Ab (Santa Cruz) and rabbit anti-GR mAb (Cell Signaling Technology, Danvers, MA). Immunostaining signals were visualized with a labeled polymer in the EnVision+ system horseradish-peroxidase kit (Dako, Carpinteria, CA). Liver infiltrating macrophages and neutrophils were detected with rat anti-CD11b Ab (BD Biosciences, San Jose, CA) and rat anti-Ly6G Ab (Bio-Rad, Hercules, CA), respectively. Signals were visualized with secondary Alexa Fluor 488 anti-rat IgG. Results were scored semi-quantitatively by blindly counting the number of positive cells in 10 HPF/section (x400).
Rabbit anti gr mab
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit anti-GR mAb is a monoclonal antibody that specifically recognizes the glucocorticoid receptor (GR) protein. It can be used for the detection and analysis of GR in various experimental applications.
Automatically generated - may contain errors
2 protocols using rabbit anti gr mab
1
Immunohistochemical Evaluation of RXFP1 and GR
2
Caudatin Regulates GR and YAP in MDA-MB-231 Cells
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After caudatin (100 µM) treatment for 24 h, MDA-MB-231 cells were fixed with 3.7% paraformaldehyde for 20 min, permeabilized with 0.5% Triton X-100 for 15 min, blocked with 3% bovine serum albumin (BSA) for 60 min, stained with a rabbit anti-GR mAb (#12041, Cell Signaling Technology, Danvers, MA, USA) and mouse anti-YAP mAb (sc-101199, Santa Cruz Biotechnology, Dallas, TX, USA), followed by staining with an anti-rabbit Alexa 488-conjugated secondary antibody (A32731) and anti-mouse Alexa 555-conjugated secondary antibody (A32727) (ThermoFisher, Waltham, MA, USA). Finally, the nuclei of cancer cells were stained with 4′, 6-diamidino-2-phenylindole (DAPI), and GR and YAP were visualized with a fluorescence microscope (Lionheart, Biotek, VT, USA).
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