3 iodo 7 azaindole

K₂[PtCl₄], 3-chloro-7-azaindole (3Claza), 3-iodo-7-azaindole (3Iaza), 5-bromo-7-azaindole (5Braza) and solvents (acetone, methanol, ethanol, diethyl ether, N,N′-dimethylformamide, water) were purchased from the following commercial sources - Sigma–Sigma-Aldrich Co. (Praha, Czech Republic), Acros Organics Co. (Pardubice, Czech Republic) and Fisher-Scientific Co. (Pardubice, Czech Republic). The complexes cis-[PtCl₂(3Claza)₂] (1), cis-[PtCl₂(3Iaza)₂] (2) and cis-[PtCl₂(5Braza)₂] (3) (Figure 1) were synthesized by the reactions of water solution of K₂[PtCl₄] with two molar equivalents of naza dissolved in ethanol, and characterized as reported previously [23] (link).
The RPMI 1640 medium and penicillin-streptomycin mixture were purchased from Lonza (Verviers, Belgium). Phosphate-buffered saline (PBS), fetal bovine serum (FBS), phorbol myristate acetate (PMA), Auranofin (98%≤), erythrosin B, and Escherichia coli 0111:B4 lipopolysaccharide (LPS) were purchased from Sigma-Aldrich (Steinheim, Germany). Cell Proliferation Reagent WST-1 was obtained from Roche (Mannheim, Germany). Instant ELISA Kits (eBioscience, Vienna, Austria) were used to evaluate the production of TNFα and IL-1β.

The chemicals K₂[PtCl₄] was purchased from Precious Metals Online, while KI, 7-azaindole, 3-chloro-7-azaindole, 3-bromo-7-azaindole, 3-iodo-7-azaindole, 4-chloro-7-azaindole, 4-bromo-7-azaindole, 5-bromo-7-azaindole, 4-chloro-2-methyl-7-azaindole, cisplatin, oxaliplatin, reduced glutathione, guanosine 5′-monophosphate disodium salt hydrate, ethidium bromide, tris(hydroxymethyl)aminomethane, calf thymus DNA, Roswell Park Memorial Institute medium, fetal calf serum, glutamine, penicillin and streptomycin, and solvents (methanol, diethyl ether, n-octanol, DMF-d₇, D₂O) were supplied by Sigma-Aldrich Co. and Fisher-Scientific Co.