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41 protocols using p benzoquinone

1

Synthesis of Polymer Nanocomposites

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TiO2 (nanopowder, 21 nm), ZnO (nanopowder, 100 nm), 2-VP, 4-VP, MO, BA, isopropyl alcohol (IPA) as solvent, benzoyl peroxide (BPO) as initiator, and p-benzoquinone and ascorbic acid as scavengers were purchased from Sigma-Aldrich. All reagents were used as received without further purification.
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2

Compound Preparation for Biological Assays

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Curcumin, azathioprine, resveratrol, catechin hydrate, baicalin hydrate, L-canavanine, 4-nitropyridine N-oxide, p-benzoquinone, esculin hydrate, cinnamaldehyde, and ciprofloxacin were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Stock solutions of ciprofloxacin and the compounds were prepared in dimethyl sulfoxide (pure DMSO), sterile water, or hydrochloric acid according to the manufacturer’s recommendations.
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3

Synthesis of Cd-Se Quantum Dots

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Cadmium acetate (Cd(OAc), 99.995%, Sigma), sodium selenite (Na2SeO3, 99%, Sigma), diethylenetriamine (99%, Sigma), TiO2 nanoparticles (Sigma-Aldrich, Aeroxide® P25, CAS: 13463-67-7), rhodamine B (RhB, >95%, Sigma), tert-butanol (t-BuOH, >99.5%, Sigma), p-benzoquinone (>98%, Sigma) and ethanol (>99%, anhydrous) were used as received without additional purification. All solutions were prepared using Milli-Q water (18.2 MΩ·cm, Millipore) as the solvent.
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4

Synthesis and Characterization of Nanoparticles

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Acetyl trimethylammonium bromide (C19H42BrN, purity >99.9%), hydrochloric acid (HCl, 37 wt%), sodium hydroxide (NaOH, purity >99.9%), silicon dioxide (SiO2, purity >99.8%), ammonium titanyl oxalate monohydrate ((NH4)2TiO(C2O4)2·H2O, purity: 98%), ethanol (C2H6O, purity ≥99.9%), nickel nitrate (Ni(NO3)2, purity> 99.8%) were purchased from Merck Company. Phenol red (C19H14O5S, purity: 98%), tert-butanol alcohol (C₄H₁₀O, purity ≥99.5%), p-benzoquinone (C6H4(=O), purity >98%), silver nitrate (AgNO3, purity ≥99%), and ammonium oxalate ((NH4)2C2O4·H2O, purity ≥99.99%) were provided from Sigma-Aldrich Company. The stock solution of the Phenol red dye was prepared daily in doubly distilled water (DDW). All working solutions were prepared by diluting the stock with DDW.
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5

Electrochemical Biosensor for COVID-19 Antigen Detection

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CPE (254 μm thick, type Spectracarb 2050A-1050, Fibers Technology), Ag/AgCl reference electrode (type RE-1CP, ALS Ltd.), Pt electrode (99.999%, 1 mm diameter, Holland-Moran Ltd), 3CLpro enzyme (Recombinant derived from Escherichia coli, ab277614, ABCAM), 3CLpro specific antibody (Rabbit-derived polyclonal, NBP3-07062, Novous Biological), 3CLpro substrate (peptide, KTSAVLQSGFRKME, Sigma-Aldrich), GFP (Recombinant derived from Escherichia coli, ab84191, ABCAM), myoglobin antibody (Monoclonal rabbit-derived, ab77232, ABCAM), HIV-2 Protease (Recombinant derived from Escherichia coli, ab84117, ABCAM), CA-15.3 (Recombinant derived from Escherichia coli, ab80082, ABCAM), Human TMPRSS2 protein (Recombinant derived from Wheat germ, ab112364, ABCAM), MERS-CoV 3CL Protease (Recombinant derived from Escherichia coli, E-719, Novous Biological), SARS-CoV 3CL Protease (Recombinant derived from Escherichia coli, E-718, Novous Biological), Chymotrypsin protein (Native human, ab90927, ABCAM), p-benzoquinone (reagent grade, Sigma-Aldrich), Acetone (9005-68, J.T.Baker), Isopropanol (IPA, 9079-05, J.T.Baker), Deionized water (DIW, 18 MΩ·cm), PBS (40 mM NaCl, 10 mM phosphate buffer, and 3 mM KCl, pH 7.4; Sigma-Aldrich), Disodium hydrogen phosphate (S7907, Sigma-Aldrich), COVID-19 antigen saliva test (P2004s, GenSure). For protein sequences, see Supplementary Table 1.
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6

Preparation of Mutant TrxR1 Enzymes

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Thioredoxin reductase 1 (TrxR1) from rat and human point-mutated TrxR1 species (in which Sec is mutated to Cys or Ser) were prepared according to previously reported methods [17 (link),32 ,33 (link)]. Reduced glutathione (GSH), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), nicotinamide adenine dinucleotide phosphate hydrogen (NADPH), biotin polyethyleneoxide iodoacetamide (BPI), 5-hydroxy-1,4-naphthoquinone (juglone), 9,10-phenanthrenequinone (9,10-PQ), auranofin and p-benzoquinone (BQ) were purchased from Sigma (St. Louis, MO, USA). BQ, juglone and 9,10-PQ was prepared freshly for every experiment by dissolving these in dimethyl sulfoxide (DMSO) to give stock solutions, with these then diluted into the relevant reaction systems. All other reagents were of highest possible quality. High purity (MQ) water was obtained from a Milli-Q System (Millipore, Bedford, MA).
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7

Synthesis and Characterization of Dye-Doped Nanoparticles

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All chemicals were of analytical grade and the highest purity available. AgNO3, Na3PO4, HNO3, phenol, p-benzoquinone, tert-butanol (t-BuOH), and ethylenediamine tetraacetate (EDTA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). MB, methyl red (MR), acid blue 1, and rhodamine B (RhB) were purchased from Invitrogen (Eugene, OR, USA). Milli-Q deionized water was used to prepare all solutions in all experiments.
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8

Radical Degradation of Organic Compounds

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Benzoic acid (BA, CAS 65–85-0) and 3-methyl-1-cyclohexanecarboxylic acid (3mCHA, CAS 13293–59) used in the sample preparation were from Sigma Aldrich Canada along with deionized water (Milli-Q/Milli-RO Millipore system, Milford, USA). Formic acid (CAS 64–18-6, Fisher Scientific Canada), methanol (Optima, CAS 67–56-1, Fisher Scientific Canada), and dichloromethane (DCM, CAS 75–09-2, Acros NJ, USA) were the solvents used for solid-phase extraction (SPE) and Fourier-transform infrared spectroscopy (FTIR). Oasis HLB (3 cc, 540 mg, P/N 186004134) cartridges were purchased from Waters (Milford, USA).
LCMS radical degradation product standards including; salicylic acid, phenol, catechol, p-benzoquinone, pyrogallol and mineralization product standards including oxalic and fumaric acid were obtained from Sigma Aldrich (Oakville, Ontario) to confirm the OH degradation and pyrolysis products after sonication.
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9

Synthesis and Characterization of Chitosan-Based Nanocomposites

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Chitin (acetylation degree > 95%), p-benzoquinone (pBQ; purity ≥ 94%), 3,5-Dinitrosalicylic acid (DNS; assay 98%), potassium persulfate (assay 98%), lysozyme enzyme (assay ≥ 95%), 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide (assay ≥ 97%), and dimethyl sulfoxide anhydrous (DMSO; assay ≥ 99.9%) were acquired from the Sigma Aldrich Company (Darmstadt, Germany). Zinc oxide NPs (99.8%, 50 ± 10 nm) were procured from Aladdin Chemical Co., Ltd. (Shanghai, China). Ethylene diamine (EDA; assay ≥ 94%) was imported from Oxford Lab Fine Chem. LLP. (Mumbai, India). Acetic acid (purity 99.8%), sodium hydroxide (purity 98%), and ethanol (98%) were purchased from Loba Chemie (Maharashtra, India).
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10

Synthesis of Graphene Oxide-Based Nanomaterials

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Titanium (IV) isopropoxide (TTIP, 97%, Sigma-Aldrich, St. Louis, MO, USA), acetylacetone (AcAc, ≥99%, Honeywell, Charlotte, NC, USA), absolute ethanol (EtOH, p.a., Grammol, Zagreb, Croatia), urea (99.5%, Sigma-Aldrich, St. Louis, MO, USA), graphene oxide water dispersion (0.4 wt.% concentration, Graphenea, Cambridge, MA, USA), ascorbic acid (p.a., Sigma-Aldrich, St. Louis, MO, USA), ciprofloxacin (CIP, 98%, Acros Organics, Waltham, MA, USA), diclofenac sodium salt (DCF, Sigma-Aldrich, St. Louis, MO, USA), salicylic acid (SA, p.a., Grammol, Zagreb, Croatia), p-Benzoquinone (≥99%, Sigma-Aldrich, St. Louis, MO, USA), formic acid (≥95%, Sigma-Aldrich, St. Louis, MO, USA) and methanol (≥99.9%, Sigma-Aldrich, St. Louis, MO, USA) were used as received without further purification. Deionized water of ultrapure quality (electrical conductivity of 0.055 µS∙cm−1 at 25 °C) was used throughout the experiments.
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