The Y-maze apparatus consisted of 3 arms, 32 cm (long) 610 cm (wide) with 26-cm walls (San Diego Instruments, San Diego, CA). The test was performed as previously described in details in our publications39 (link),40 (link).
26 cm walls
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The 26-cm walls are a structural component designed for use in laboratory environments. This product provides a physical barrier with standardized dimensions to assist in the organization and containment of laboratory equipment and materials.
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7 protocols using 26 cm walls
1
Y-Maze Apparatus for Spatial Memory
2
Y‐maze Spontaneous Alternation Assay
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The Y‐maze apparatus consisted of 3 arms, 32 cm (long) 610 cm (wide) with 26‐cm walls (San Diego Instruments, San Diego, CA). As previously described13 , 14 , testing was always performed in the same room and at the same time to ensure environmental consistency as previously described. After introduction to the center of the Y‐maze, the animal is allowed to freely explore the three arms for 5 min and the sequence and total number of arms entered was video‐recorded in order to calculate the percentage of alternation. Any entry into an arm was considered valid if all limbs were within the arm. An alternation was defined as three consecutive entries in three different arms (1,2,3 or 2,3,1, etc.). The percentage alternation score was calculated using the following formula: total alternation number/total number of entries−2) × 100.
3
Y-Maze Spontaneous Alternation Behavior
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The Y-maze apparatus consisted of three arms 32 cm (long) 610 cm (wide) with 26-cm walls (San Diego Instruments, San Diego, CA) and testing was performed as previously described [17 (link)–19 ]. Briefly, each mouse was placed in the center of the Y-maze and allowed to explore freely for five minutes to measure spontaneous alternating behavior. The sequence and total number of entries were video-recorded. An entry into an arm was considered valid if all four paws entered the arm. An alternation was defined as three consecutive entries into three different arms (1, 2, 3, or 2, 3, 1, etc.). Percentage of alternation was calculated using the following formula: total alternation number/total number of entries−2) × 100.
4
Measuring Spontaneous Alternation in Y-Maze
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The Y-maze apparatus consisted of three arms 32 cm (long) and 610 cm (wide) with 26-cm walls (San Diego Instruments, San Diego, CA). Testing was always performed in the same room and at the same time to ensure environmental consistency as previously described12 (link),13 (link). Briefly, each mouse was placed in the center of the Y-maze and allowed to explore freely during a 5-min session as a measure of spontaneous alternating behavior. The sequence and total number of arms entered were video-recorded. An entry into an arm was considered valid if all four paws entered the arm. An alternation was defined as three consecutive entries into three different arms (1, 2, 3, or 2, 3, 1, etc.). Percentage of alternation was calculated using the following formula: total alternation number/total number of entries−2) × 100.
5
Y-Maze Exploration Behavioral Assay
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The Y-maze apparatus consisted of three arms 32 cm (long) 610 cm (wide) with 26-cm walls (San Diego Instruments, San Diego, CA). Testing was always performed in the same room and at the same time to ensure environmental consistency as previously described29 (link)33 (link). Briefly, each mouse was placed in the center of the Y-maze and allowed to explore freely through the maze during a 5-min session. The sequence and total number of arms entered were video recorded. An entry into an arm was considered valid if all four paws entered the arm. An alternation was defined as three consecutive entries in three different arms (i.e. 1, 2, 3 or 2, 3, 1, etc). The percentage alternation score was calculated using the following formula: Total alternation number/ (total number of entries-2)*100.
6
Y-Maze Spontaneous Alternation Behavior
7
Y-Maze Exploration Behavior Assessment
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The Y‐maze apparatus consisted of 3 arms, 32 cm (long) 610 cm (wide) with 26‐cm walls (San Diego Instruments, San Diego, CA). As previously described (Lauretti, Iuliano, et al., 2017 ; Li, Barrero, Merali, & Praticò, 2017 ), testing was always performed in the same room and at the same time to ensure environmental consistency. After introduction to the center of the Y‐maze, the animals freely explored the three arms for 5 min and the sequence and total number of arms entered was video‐recorded to calculate number of entries and percentage of alternation. Entry into an arm was considered valid if all limbs were within the arm, while alternation was defined as three consecutive entries in three different arms (1, 2, 3 or 2, 3, 1, etc.). The percentage of alternation was calculated using the following formula: total alternation number/total number of entries–2) × 100.
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