The newly acquired specimens used for description and photography were collected from Nan Province in Northern Thailand. Larvae were collected from cobble in moderate to fast-flowing areas. Mature larvae were reared using earthenware pots connected to an air supply (Figure 8C) until emergence of winged stages. The specimens that have been used to obtain initial COI data were retrieved from L.M.J.’s research collection. The chorionic structure was investigated by drying the eggs, coating them with gold, and observing them with a FEI Quanta 450 Scanning Electron Microscope (SEM). The external structures were prepared on permanent slides using Euparal® as a medium and observed by light microscopy. Final plates were prepared with Adobe Photoshop® CC 2020. Holotype and paratype specimens of the new species are deposited in the collections of the Zoological Museum at Kasetsart University in Bangkok, Thailand [ZMKU ] and the Museum of Zoology in Lausanne, Switzerland [MZL ]. L.M.J.’s materials are currently stored in trays with other specimens used for DNA barcoding, and these units will be deposited in the Purdue University Entomological Research Collection, West Lafayette, Indiana, United States of America [PERC ]. Our species hypotheses are based on the convergence of the morphological species concept and the phylogenetic species concept.
Quanta 450 scanning electron microscope
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Quanta 450 Scanning Electron Microscope (SEM) is a versatile instrument designed for high-resolution imaging and analysis of a wide range of samples. It utilizes a focused electron beam to scan the surface of the sample, producing detailed images that reveal the morphology, composition, and topography of the specimen. The Quanta 450 SEM is capable of operating under high-vacuum, low-vacuum, and environmental conditions, making it suitable for a variety of applications.
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Lab products found in correlation
Conductive silver paint, by Ted Pella (1 mentions)
Quanta 450 electron microscope, by Thermo Fisher Scientific (1 mentions)
Em cpd300 automated critical point dryer, by Leica (1 mentions)
Em scd050, by Leica (1 mentions)
K850 critical point dryer, by Quorum Technologies (1 mentions)
High purity chitosan, by Merck Group (1 mentions)
14 protocols using quanta 450 scanning electron microscope
1
Rearing and Describing a New Aquatic Insect Species
2
Quantifying HA-Particle Surface Composition
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An investigation of the effect of various volume fractions of micro/nano HA particles on the surface composition of the composites was carried out using the Quanta 450 scanning electron microscope (FEI) equipped with an Apollo XL Silicon Drift Detector 1 EA EDS extension (EDAX Inc., Mahwah, NJ, USA) at magnification 5,000× and accelerating voltage 20 kV. Prior to the EDS analyses, the samples were attached by carbon paste on an alumina stub and carboncoated on a K550X (EMITech, Inc., Fall River, MA, USA) sputter coater in an argon atmosphere. The atomic fractions (at %) of chemical elements (namely C, O, Si, P, and Ca) were characterized in order to verify their surface incorporation. Ten 25 μm × 25 μm areas were analyzed at each HA volume fraction level.
3
Sample Preparation for SEM Imaging
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Samples for SEM analysis were subjected to gradual cooling (−20°C to −80°C) after fixation with 10% glutaraldehyde solution for 6–8 h. After the fixed samples were completely frozen, the samples were subjected to lyophilization for 48 h at about −105°C/100 mTorr vacuum. Freeze-dried samples were attached to an aluminum sample stub with conductive silver paint (Ted Pella, Inc., Redding, CA) and tightened using copper tape. Samples were then subjected to sputter-coating with a layer of ∼10 nm gold film before imaging by SEM. SEM images were acquired using an FEI Quanta 450 Scanning Electron Microscope at different magnifications. The accelerating voltage applied was 10 kV, and images were acquired using a dwell time of 30–60 μs.
4
Scanning Electron Microscopy of Biomaterial Scaffolds
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A Quanta 450 electron microscope (FEI, Hillsboro, OR, USA) was used for the scanning electron microscopy (SEM) of the scaffold in the high vacuum mode. An ion sputter (Emitech K550X, Quorum Technologies, Kent, UK) was used for the coating of the samples with a thin layer of gold. The adsorption of various components from the blood plasma, SBF, and PBS following exposure was qualitatively characterized via energy-dispersive spectrometry (EDS) using a Quanta 450 scanning electron microscope (FEI, Hillsboro, OR, USA) equipped with an Apollo XL Silicon Drift Detector 1 EA EDS extension (EDAX Genesis system, Mahwah, NJ, USA) at a magnification of 1000× and an accelerating voltage of 12.5 kV. Prior to the EDS analysis, dried samples (n = 5) were sputter-coated with carbon in an ion sputter (Emitech K550X, Quorum Technologies, Kent, UK). The weight fractions of the chemical elements incorporated within the dried samples before and after exposure in the media were characterized.
5
Characterizing C. difficile Biofilm Structure
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The C. difficile biofilm structure was characterized using Scanning electron microscopy (SEM), as described in Pantaleon et al., 2014 (Pantaléon et al., 2014 (link)), with some modifications. C. difficile strains were grown onto coverslips with a diameter of 13 mm, as described above (please see the section 2.2) in order to analyze biofilm production after 72 h. Samples were fixed in 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer for 30 min, followed by a post-fixation with 1% osmium tetroxide for 15 min. Samples were dehydrated in a series of ethanol concentrations (15%, 30%, 50%, 70%, 90% and 100%), critical point‐dried in CO2 and mounted on specimen stubs. Stubs were sputtered with a thin layer of gold using a Balzer’s apparatus and examined in a Quanta 450 scanning electron microscope (FEI Company), at the Central analytical at the Universidade Federal do Ceará (UFC).
6
Scanning Electron Microscopy of Uncoated Wood
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A FEI Quanta 450 Scanning Electron Microscope with a voltage of 8 kV on low vacuum was used. Spot size was 4.5, chamber pressure was 50–80 Pa with working distances of 7.0–10.0 mm. The SEM samples were carefully shaved with a razor in order to get a surface which was flat, enabling better visualisation of the wood cells. It was ensured that not too much material was shaved off from the surface, in order not to compromise the results. No coating was required since a high vacuum was not used.
7
Scanning Electron Microscope Imaging
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Scanning Electron Microscope (SEM) images were recorded on an FEI Quanta 450 Scanning Electron Microscope. Electron images were taken using a large field detector (LFD) under low vacuum (LV) mode in order to avoid charging of the samples. The other parameters (voltage, spot size, pressure, and working distance) were modified depending on the sample with average values of HV 6.0 kV, spot size 4.5, pressure 100 Pa and working distance of 7.9 mm.
8
Elemental Composition Analysis Using SEM-EDS
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For elemental concentration, EDS was used on the Quanta 450 scanning electron microscope (FEI, Hillsboro, OR, USA) equipped with a Si(Li) Apollo XL Silicon Drift Detector with an FET preamplifier (EDAX Inc., Mahwah NJ, USA) at a magnification of 1000× and an accelerating voltage of 12.5 kV. Prior to the EDS analyses, samples were attached by carbon tape to an alumina stub and were gold-coated on a K550X (Quorum Technologies Ltd, Ashford, UK) sputter coater in an argon atmosphere. The weight and the atomic fractions of the chemical elements were characterized. Five measured fields were taken for each sample at a magnification of 1000× (measured area of 85.456 µm2, i.e., a total of 427.331 µm2 for each sample). On each sample, the EDS map was taken at 1000× magnification. Data acquisition was performed on EDAX TSL OIM software, v. 7.0 with ZAF corrections.
9
Ultrastructural Analysis of Curtobacterium luteum
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Curtobacterium luteum cells at the exponential growth phase were treated with 20 × CL (0.6 μM) of Py4 or 20 × vancomycin (2.0 μM) at 37 °C for 90 min. After centrifugation, bacterial pellets were fixed with 2.5% glutaraldehyde at 4 °C overnight, followed by washing three times with water. Dehydration was carried out with a series of graded ethanol solutions. Cells were then dried by a Leica EM CPD300 Automated Critical Point Dryer (Leica Microsystems, Wetzla, Germany) before being mounted on carbon tape and sputtered with a gold coating by a Leica EM SCD050. Images were visualized by using a FEI Quanta 450 scanning electron microscope (FEI, Hillsboro, OR, USA).
10
MRSA Membrane Morphology by SEM
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In order to investigate the effects of morusin and kuwanon G on the morphology of MRSA ATCC 43300 membrane, scanning electron microscopy (SEM) was performed according to Basri et al. (2013) with slight modifications. After 24 h incubation at 37 °C with morusin or kuwanon G (each at ½ × MIC), the treated and untreated (control) bacterial cell suspensions were centrifuged at 2000 rpm for 10 min, washed two times with 0.1 M PBS (pH = 7.2), fixed with 2.5% glutaraldehyde in 0.1 M PBS at 4 °C for 2 h, washed with PBS twice and post-fixed with 1% osmium tetroxide in sterile water at room temperature for 4 h. The bacterial cells were further washed two times with PBS, successively dehydrated with a series of ethanol dilutions (30%, 50%, 70% and 99.8%, 15 min for each dilution) and finally suspended in propylene oxide overnight. For imaging the samples, FEI Quanta 450 Scanning Electron Microscope was used (high vacuum mode (about 10–4 Pa), electron acceleration voltage of 12.5 V).
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