Spectrum two spectrometer

Manufactured by PerkinElmer

Sourced in United States, United Kingdom, Japan

The Spectrum Two spectrometer is a versatile laboratory instrument designed for performing spectroscopic analysis. It uses infrared spectroscopy to identify and quantify chemical compounds. The spectrometer can analyze a wide range of sample types and provide detailed information about the molecular structure and composition of materials.

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Lab products found in correlation

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Close spelling variants of this product

Spectrum TwoTM IR spectrometer (2 citations) Spectrum TwoTM FT-IR Spectrometer (7 citations) Spectrum Two FT-IR spectrometer (234 citations) Spectrum Two Fourier-transform infrared spectrometer (3 citations) Spectrum Two UATR FTIR spectrometer (9 citations) Perkin-Elmer Spectrum Two IR spectrometer (2 citations) Spectrum Two ATR-FTIR spectrometer (8 citations) Spectrum Two Infrared Spectrometer (5 citations) PerkinElmer FTIR spectrometer Spectrum Two (2 citations) FTIR-Spectrometer Spectrum Two Model (2 citations)

96 protocols using spectrum two spectrometer

Starch Structure Analysis by FTIR

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The spectra of the starch samples were obtained using a PerkinElmer^® Spectrum Two spectrometer (PerkinElmer, Houston, TX, USA) with a resolution of 4 cm⁻¹, wavelength range from 4000 to 600 cm⁻¹, and 32 scans. The starch spectra were obtained via the attenuated total reflection (ATR) method.
The effect of ball milling on the ordered structure of the starch was also evaluated using FTIR spectra. It has been reported that bands between 950 and 1100 cm⁻¹ are sensitive to changes in the starch structure, especially those at approximately 995 and 1044 cm⁻¹, where the first is commonly related to the amorphous structure of the starch and the latter to the ordered structure of the starch [15 (link),16 (link)]. Therefore, the 995:1044 cm⁻¹ intensity ratio was used to indicate the degree of order in the starch structure.

de Oliveira Barros M., Mattos A.L., de Almeida J.S., de Freitas Rosa M, & de Brito E.S. (2023). Effect of Ball-Milling on Starch Crystalline Structure, Gelatinization Temperature, and Rheological Properties: Towards Enhanced Utilization in Thermosensitive Systems. Foods, 12(15), 2924.

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Characterization of Simvastatin-DMbCD Complex

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Differential Scanning Calorimetry (DSC) analyses of simvastatin, DMbCD, simvastatin /DMbCD physical mixture (PM ) and simvastatin /DMbCD complex were carried out on a simultaneous thermal analyzer STA 449 F3 Jupiter ® (Netzsch-Gerätebau GmbH, Germany). Samples weighing between 5 and 10 mg were loaded into open aluminum pans and placed into the DSC cell. The cell had a nitrogen purge flowing at approximately 20 mL min -1 . The DSC was used to analyze the samples from 40-200 °C at a 10 °C/min heating rate. An indium pan served as reference, and all scans were performed in triplicate. The instrument was calibrated before sample analysis using an indium standard.
Fourier-transform infrared (FT-IR) analyses of the samples were performed using a Perkin-Elmer Spectrum Two spectrometer (PerkinElmer Corporation, USA). Simvastatin, DMbCD, simvastatin /DMbCD PM and simvastatin /DMbCD complex was mixed separately with IR grade KBr in the weight ratio of 100:1 for tablet preparation. Final spectra were performed in a range of 400-4000 cm -1 with 2 cm -1 resolution. All samples were analyzed in triplicate.

Gu F., Ning J., Fan H., Wu C, & Wang Y. (2018). Preparation and characterization of simvastatin/DMβCD complex and its pharmacokinetics in rats. Acta pharmaceutica (Zagreb, Croatia), 68(2).

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Characterization of TRAIL Inclusion Bodies

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First, 1 mg of dry TRAIL IBs powder and 200 of mg pure KBr were mixed, grinded and tableted. Then, FT-IR spectra were obtained from the tablet using a Spectrum Two spectrometer (PerkinElmer, USA). Each spectrum was obtained between 4000 and 400 cm⁻¹ at a resolution of 4 cm⁻¹ with 256 scans. The FT-IR spectra was processed with Fourier self-deconvolution using OMNIC software (Themofisher, USA). The second-derivative spectra of TRAIL IBs in the amide I region were obtained using the Savitzky-Golay method^{38 (link)}.

Wang Z., Zhang M., Lv X., Fan J., Zhang J., Sun J, & Shen Y. (2018). GroEL/ES mediated the in vivo recovery of TRAIL inclusion bodies in Escherichia coli. Scientific Reports, 8, 15766.

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Characterization of Bioinspired Functional Polymers

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The reactions of ROP and photopolymerization were monitored based on the transformation of functional groups such as hydroxyl, carbonyl, and alkene by FT-IR. The spectra of samples were obtained in the region of 4000–500 cm⁻¹ using a Spectrum Two spectrometer (PerkinElmer). An air background spectrum was collected before the analysis of the sample and subtracted from each sample spectrum.
The Young’s moduli of the BFP samples were measured using the CellScale UniVert mechanical tester (CellScale, Waterloo, Canada) via compression testing at a strain rate of 6.7 × 10⁻³ s⁻¹ (n = 3 per BFP formulation). Young’s modulus was calculated from the linear strain region (5–10%). The data are reported as mean ± standard deviation. Optical transmissions of the thin BFP films were measured from 300 to 1000 nm using a UV–vis spectrophotometer (Infinite 200 PRO, Tecan, Man̈nedorf, Schweiz). Differential scanning calorimetry (DSC) was carried out using DSC Q20 (TA Instrument) over a temperature range from 0 to 180 °C, increasing 10 °C/min under nitrogen. Thermogravimetric analysis (TGA) was performed using Pyris 1 TGA (PerkinElmer) from 20 to 200 °C, increasing 10 °C/min under nitrogen. High-resolution scanning electron microscopy (SEM) images were obtained using Zeiss Sigma 500. The surface of the structure was coated with iridium by an Emitech K575X sputter coater prior to imaging.

Zhu W., Pyo S.H., Wang P., You S., Yu C., Alido J., Liu J., Leong Y, & Chen S. (2018). Three-Dimensional Printing of Bisphenol A‑Free Polycarbonates. ACS applied materials & interfaces, 10(6), 5331-5339.

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Characterization of Polymeric Materials

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1 H NMR and 13 C NMR spectra of polymers in CDCl3 were recorded on a Varian 600 MHz spectrometer with TMS as an internal standard. Infrared (FT-IR) spectra were recorded by means of a PerkinElmer Spectrum Two Spectrometer. Molecular weights of polyanhydrides were determined in methylene chloride by gel-permeation chromatography (GPC) using Agilent Technologies Infinity 1260 chromatograph equipped with a refractive index detector and calibrated with polystyrene standards. Molecular weights were also calculated from 1 H NMR spectra. Thermal analyses were performed using an 822 e DSC Mettler Toledo differential scanning calorimeter. Samples were tested in temperature range from -60 °C to 250 °C at a heating rate of 10 °C/min.

Niewolik D., Bednarczyk–Cwynar B., Ruszkowski P., & Jaszcz K. (2020). Novel Biodegradable Polyanhydrides Based on Betulin Disuccinate and Sebacic Acid for Medical Purpose.

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ATR-FTIR Characterization of mcl-PHA

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Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) analysis was conducted in a Perkin Elmer Spectrum Two spectrometer. The mcl-PHA was directly placed in the ATR-FTIR cell and spectra were recorded from 4000 to 400 cm⁻¹ resolution with 16 scans at room temperature.

Meneses L., Craveiro R., Jesus A.R., Reis M.A., Freitas F, & Paiva A. (2021). Supercritical CO2 Assisted Impregnation of Ibuprofen on Medium-Chain-Length Polyhydroxyalkanoates (mcl-PHA). Molecules, 26(16), 4772.

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FTIR Analysis of Anhydrous and Hydrated Biodentine

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FTIR spectra of anhydrous and hydrated Biodentine samples were acquired using a Perkin Elmer Spectrum Two spectrometer ((Perkin Elmer, London, UK)) between 700 and 1900 cm⁻¹ wavenumbers, with 10 scans at a resolution of 4 cm⁻¹. Hydrated Biodentine samples were manually ground with an agate mortar and pestle prior to analysis.

Li Q., Hurt A.P, & Coleman N.J. (2019). The Application of 29Si NMR Spectroscopy to the Analysis of Calcium Silicate-Based Cement using Biodentine™ as an Example. Journal of Functional Biomaterials, 10(2), 25.

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Comprehensive Characterization of Material Samples

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X-ray Diffraction (XRD) analysis was conducted on a Philips X-Ray Generator (Model PW 1130) with a PW (Model 1050) goniometry (Eindhoven, The Netherlands). Scanning Electron Microscopy (SEM) analysis was performed using a Philips XL30 ESEM coupled with EDX equipment (Amsterdam, The Netherlands). The morphology and compositional features of the samples were analyzed through a Scanning Electron Microscope Philips XL30 ESEM coupled with EDX equipment. Fourier-transform infrared (FTIR) analysis was carried out using a PerkinElmer Spectrum Two spectrometer (Waltham, MA, USA) in the ATR mode in the range 400–4000 cm⁻¹. Thermogravimetric (TG) analysis was done using a Netzsch STA/409/2 (Selb, Germany) thermal analysis system at a scanning rate of 10 °C min⁻¹ in the range 25–350 °C under a nitrogen atmosphere.

, & Bocchetta P. (2020). Ionotropic Gelation of Chitosan for Next-Generation Composite Proton Conducting Flat Structures. Molecules, 25(7), 1632.

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Tetracycline Hydrochloride Release Kinetics

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The TCH release behaviors were studied in 0.1 M phosphate buffer saline (PBS) buffers with pH 7.4. The sponges were cut into round pieces with the diameter of 10 mm. The tested samples were fully immersed in a beaker containing 50 mL PBS at 37 °C and placed in a dark place. At specific time points, an aliquot of 3.5 mL was collected from each solution and the absorbance was then measured at 356 nm on a Spectrum Two Spectrometer (Perkin Elmer, Akron, OH, USA). An equivalent volume of fresh PBS buffer was replaced into the system after each sampling to maintain constant medium volume. Thus, the concentrations of released TCH obtained at different times can be calculated. As a result, the cumulative released TCH amounts can be calculated accordingly. The experiments were performed in triplicate.

Wen Y., Yu B., Zhu Z., Yang Z, & Shao W. (2020). Synthesis of Antibacterial Gelatin/Sodium Alginate Sponges and Their Antibacterial Activity. Polymers, 12(9), 1926.

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Tetracycline Hydrochloride Loading in G/SA Sponges

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TCH loadings were calculated according to the original concentration of TCH (C₀) and the concentration of unloaded TCH (C₁) determined by a Spectrum Two Spectrometer (Perkin Elmer, Akron, OH, USA) at the monitoring wavelength of 356 nm. TCH loadings (W) in the G/SA sponges were calculated using the following equation:

W = \frac{C_{0} - C_{1}}{A} \times V

where V is the volume of total TCH solution, A is the area of the G/SA sponge.

Wen Y., Yu B., Zhu Z., Yang Z, & Shao W. (2020). Synthesis of Antibacterial Gelatin/Sodium Alginate Sponges and Their Antibacterial Activity. Polymers, 12(9), 1926.

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