C57bl 6j

Manufactured by GemPharmatech

Sourced in China

The C57BL/6J is a widely used laboratory mouse strain. It is an inbred strain, meaning it has been bred to maintain a consistent and uniform genetic background. The C57BL/6J strain is commonly used in research due to its well-characterized genetics and physiological characteristics.

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Lab products found in correlation

Pde6bem1cin, by GemPharmatech (2 mentions) C57bl 6j mice, by GemPharmatech (2 mentions) C57bl 6 tg cag egfp 1osb j mice, by Jackson ImmunoResearch (1 mentions) C57bl 6j, by Research Diets (1 mentions) Dba 1 j mice, by GemPharmatech (1 mentions) Balb c mice, by GemPharmatech (1 mentions) Dextran sulfate sodium (dss), by MP Biomedicals (1 mentions) Nω nitro l arginine methyl ester hydrochloride l name, by Merck Group (1 mentions) Mle 12 cells, by American Type Culture Collection (1 mentions) B16f10 cells, by American Type Culture Collection (1 mentions)

28 protocols using c57bl 6j

Orthodontic Movement in CB2 Knockout Mice

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Cnr2 knockout mice (CB2^−/−, C57BL/6 J background) were obtained from Gempharmatech Co., Ltd. A total of 15 male CB2^−/− mice (22 to 23 g) and 15 male wild-type mice (WT, C57BL/6 J, 22 to 23 g) aged 6 weeks were used in this study. The traffic light breeding strategy was used to obtain heterozygous mice. All mice were housed in temperature- and humidity-controlled cages with a 12:12 h light: dark cycle and had free access to food and water. They were fed a soft diet to minimize any discomfort or displacement of the orthodontic appliance. Mouse genotyping protocol was performed by polymerase chain reaction (PCR) to identify CB2^−/− and WT mice from the offspring of heterozygous mice. The PCR protocol was provided by Gempharmatech Co., Ltd [16 (link), 17 (link)]. The mice were randomly allocated into two groups (Table 1)-WT orthodontic movement groups (WT OTM):0,7 and 14 days; CB2^−/− orthodontic movement groups (CB2^−/− OTM): 0, 7 and 14 days.

Table 1

Distribution of samples for each group

OTM (days)	WT (mice)	CB2(mice)
0	5^a	5^a
7	5	5
14	5	5

^aWithout force application

Fan D.Y., Zhai H.Y., Zhao Y., Qiao X., Zhu D.C., Liu H.J, & Liu C. (2024). The role of cannabinoid receptor 2 in bone remodeling during orthodontic tooth movement. BMC Oral Health, 24, 23.

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Immunological Experiment with Genetically Modified Mice

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BLAB/C (female, 6–8 weeks, 18–22 g), C57BL/6J (female, 6–8 weeks, 18–22 g), and C57BL/6J (born within 24 h) were purchased from Jiangsu Gempharmatech Co, Ltd cGAS^−/− mice were obtained from Prof. Cheng Qian of Nanjing Medical University. Mice were maintained in an animal facility under standard laboratory conditions for 1 week before the experiments and provided water and standard chow. The animal welfare and experimental procedures were approved by the Institutional Animal Ethnical and Welfare Committee of Nanjing University (IACUC-D2003011, Nanjing, China). All efforts were made to reduce the number of animals used and to minimize animals’ suffering.

Luan C., He Y., Liu W., Rong Y., Gao J., Xu K., Yu H., Hu Y., Zhang J., Chen K, & Guo W. (2023). PCSK9 inhibition interrupts the cross-talk between keratinocytes and macrophages and prevents UVB-induced skin damage. The Journal of Biological Chemistry, 299(7), 104895.

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Induction of Peritoneal Adhesions in Mice

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The Jinling Hospital’s Animal Investigation Ethics Committee authorized all of the animal care and experimental protocols, which were carried out in strict accordance with the Chinese Guidelines for the Care and Use of Laboratory Animals (Ministry of Science and Technology [2006] file no. 398). Pain was kept to a minimum by doing all procedures under anesthesia. Adhesion induction procedures were performed on wildtype B6 (C57BL/6J; GemPharmatech Co. Ltd., China) mice at 6–8 weeks. On the median line, a skin incision was made along the length of the abdomen. Based on the length of the peritoneum, a comparable midline abdominal incision was created in the peritoneum. The peritoneum was gently folded to the right and compressed with a hemostat. An ischemia button was inserted into the right side of the peritoneal wall after a tiny section of peritoneum (about 5 mm in diameter) was clamped with hemostatic forceps, the bottom of which was ligated with 4-0 silk (Suzhou Medical Co. Ltd., China), and the forceps were then released. Optionally, a light scrubbing of56 the button (20 times) and the adjacent liver, cecum, small intestine, and large intestine (7 times) could be performed with a surgical brush (depending on the desired severity of adhesions). The peritoneum and skin were closed with 4-0 silk sutures^{[25 ]}.

Li S., Huang J., Xu Z., Liu Y., Ren H., Li Z., Chen C., Chen K., Wu* X, & Ren* J. (2023). Melt electrowriting-printed peritoneal scaffold prevents peritoneal adhesion and facilitates peritoneal repair. International Journal of Bioprinting, 9(3), 682.

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Liver Fibrosis Mouse Model Development

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C57BL/6 J and B6/JGpt-Ccr2^em8Cd6657/Gpt were obtained from GemPharmatech. C57BL/6J-TgN (Chicken-β-actin-LUC) ZLFILAS mice were obtained from Shanghai Sciencelight Biology Science&Technology. C57BL/6-Tg (CAG-EGFP) 1Osb/J mice were obtained from the Jackson Laboratory. All mice were housed under standard conditions as previously described [20 (link)]. The 8- to 12-week-old and sex-matched mice were used for the experimental procedures. For liver fibrosis models, mice were intraperitoneally injected with CCl₄ (1 mL/kg; 1:10 [v/v] in olive oil, Sangon Biotech) twice a week for 4 weeks. The animals were sacrificed 72 h after the final CCl₄ injection, and serum and whole livers were collected for biochemical, histological, and molecular analyses as previously described [31 (link)]. For the transplantation experiment, mice were treated with CCl₄ for 4 weeks with eight injections, and 1 × 10⁶ BM-MSCs were transplanted into mice 24 h after the final injection. The mice were then continually injected with CCl₄ for another week until use. All animal experiments were performed in accordance with legal regulations and approved by a local ethics committee.

Li Y.H., Shen S., Shao T., Jin M.T., Fan D.D., Lin A.F., Xiang L.X, & Shao J.Z. (2021). Mesenchymal stem cells attenuate liver fibrosis by targeting Ly6Chi/lo macrophages through activating the cytokine-paracrine and apoptotic pathways. Cell Death Discovery, 7, 239.

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Rarα Knockout Mice Characterization

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C57/BL6 mice were purchased from the Guangdong Medical Laboratory Animal Center (Guangzhou, China). Rarα heterozygous knockout mice on the C57BL/6J background were purchased from Gempharmatech Co.,Ltd (Nanjing, China). The mice were housed in individually ventilated cages at 22 ± 1 °C for a 12 h light-dark cycle and ad libitum access to water and food. Rarα homozygous knockout mice were obtained from the mating of Rarα heterozygous parental mice at 1:1. The day after partum was designated as 0.5 days postpartum (dpp). For genotyping, genomic DNA was isolated from tail biopsies by PCR with a combination of two primers. The primers were synthesized at BGI Genomics (BGI-Tech, Shenzhen, China) and the sequences are listed in Supplementary Table S1. The females were observed continuously daily afternoon from 17 dpp until the vaginal opening and recorded. To avoid male interference, monitored females were caged individually at 21 dpp. All experiments were conducted under the Guidelines of the Animal Care and Use Committee of South China University of Technology. Unless otherwise stated, the reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Zheng N., Zhang W., Zhang X., Li B., Wu Z., Weng Y., Wang W., Miao J., Yang J., Zhang M, & Xia W. (2023). RA-RAR signaling promotes mouse vaginal opening through increasing β-catenin expression and vaginal epithelial cell apoptosis. Reproductive Biology and Endocrinology : RB&E, 21, 36.

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Ginsenoside CK Improves Obesity in ob/ob Mice

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Male C57BL/6J (wild-type mice) and ob/ob (B6/JGpt-Lep^em1Cd25/Gpt) mice were obtained from GemPharmatech Co., Ltd. (Nanjing, China). At 5 weeks of age, mice were housed in a temperature-controlled facility (21 °C, 12 h light/12 h dark cycle, 60–70% humidity). Standard laboratory food (60% cereals, 33% protein, and 3% oil) and water were given ad libitum. After 1 week of feeding, the mice were subjected to the different treatments at 6 weeks of age (25 ± 5 g). Body weight and blood glucose were measured weekly. All experiments were approved by the Animal Care and Use Committee of Northeast Normal University (SYXK 2018-0015). At the end of the experiments, serum and organ samples were collected for the determination of biochemical parameters. Further details about the biochemical analysis and histology methods are included in the Supporting Information (Appendix A).
The mice were treated with 20 mg/kg of the ginsenoside CK or DMSO (vehicle) through i.p. injection once a day for five weeks. The mice were orally administered 150 mg/kg orlistat as a positive control.

Yang S., Liu T., Hu C., Li W., Meng Y., Li H., Song C., He C., Zhou Y, & Fan Y. (2022). Ginsenoside Compound K Protects against Obesity through Pharmacological Targeting of Glucocorticoid Receptor to Activate Lipophagy and Lipid Metabolism. Pharmaceutics, 14(6), 1192.

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Healthy Mice Feeding and Care

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In this study, 6–8-week-old male healthy mice (C57BL/6J) were used (GemPharmatech Co., Ltd., Nanjing, China). All mice were kept in specific-pathogen-free (SPF) feeding rooms in the Laboratory Animal Center and Animal Laboratory of Nephrology, Shanxi Provincial People's Hospital (Shanxi, China) at a temperature of 24 ± 2°C and a humidity of 40–70% and under 24 h light-dark cycle condition. Mice were fed with sterile food and water, and autoclaved bedding litter in the cage was changed two times a week. Under the permission of the Committee on the Ethics of Animal Experiments of Shanxi University (Shanxi, China), all experiments were performed in accordance with published National Institutes of Health guidelines.

Yang R., Shan S., An N., Liu F., Cui K., Shi J., Li H, & Li Z. (2022). Polyphenols from foxtail millet bran ameliorate DSS-induced colitis by remodeling gut microbiome. Frontiers in Nutrition, 9, 1030744.

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Retinitis Pigmentosa Mouse Model Study

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There were two groups of mice, including the rd1 mice group with retinitis pigmentosa (rd1, Pde6bem1Cin purchased from Gem Pharmatech Co., Ltd., Nanjing, China) and the control mice group (C57BL/6J, purchased from Gem Pharmatech Co., Ltd.). Other variables were controlled for both groups, such as equal numbers of males and females. The animals used in this study were all males. All samples were collected between 1:00 pm and 2:00 pm, to prevent potential effects from circadian rhythms and the onset of light. The mice were be euthanized by cervical subluxation after anesthesia.

Zhou Y., Sheng Y., Pan M., Tu J., Zhao X., Ge Q, & Lu Z. (2023). Spatial Transcriptomic Analysis Reveals Regional Transcript Changes in Early and Late Stages of rd1 Model Mice with Retinitis Pigmentosa. International Journal of Molecular Sciences, 24(19), 14869.

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Retinitis Pigmentosa Mouse Model Study

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Mice Models for Inflammatory Bowel Disease

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C57BL/6J and Il10^−/− mice were bought from GemPharmatech company (Jiangsu, China). Six-to eight-week-old male and female C57BL/6J wild-type mice and approximately 20 weeks old Il10^−/− mice were housed at specific pathogen-free (SPF) facilities at the Sixth Affiliated Hospital of Sun Yat-Sen University and Guangzhou Ruige Biological Technology Co., Ltd. Mice were handled in accordance with the protocols approved by the Institutional Animal Care and Use Committee (IACUC) at the Sun Yat-Sen University and Guangzhou Ruige Biological Technology Co., Ltd. The approval numbers for the studies involving mice were IACUC-2020071402 and IACUC-20211021-027.

Zeng W., Wu J., Xie H., Xu H., Liang D., He Q., Yang X., Liu C., Gong J., Zhang Q., Luo Z., Chen Y., He Z, & Lan P. (2024). Enteral nutrition promotes the remission of colitis by gut bacteria-mediated histidine biosynthesis. eBioMedicine, 100, 104959.

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