Pelp1 antibody a300 180a

Manufactured by Fortis Life Sciences

Sourced in United States

The PELP1 antibody (A300-180A) is a research-use only product manufactured by Fortis Life Sciences. It is designed to detect the PELP1 protein in various biological samples using techniques such as Western blotting and immunohistochemistry. The antibody has a molecular weight of approximately 120 kDa.

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Lab products found in correlation

Ki67 antibody ab1667, by Abcam (2 mentions) Mda mb 231, by American Type Culture Collection (2 mentions) Mcf 7, by American Type Culture Collection (2 mentions) Fulvestrant, by MedChemExpress (2 mentions) Zr 75, by American Type Culture Collection (2 mentions) C myc, by Cell Signaling Technology (1 mentions) 4e bp1, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) P 4ebp1, by Cell Signaling Technology (1 mentions) Hcc1806, by American Type Culture Collection (1 mentions) Bt549, by American Type Culture Collection (1 mentions) Mda mb 468, by American Type Culture Collection (1 mentions) Hcc1937, by American Type Culture Collection (1 mentions) Mda mb 453, by American Type Culture Collection (1 mentions) Sum159, by American Type Culture Collection (1 mentions) β actin a2066, by Merck Group (1 mentions) Iose80, by American Type Culture Collection (1 mentions) Ki67 antibody ab 16667, by Abcam (1 mentions) Mcf 7, by Merck Group (1 mentions) Tamoxifen, by Merck Group (1 mentions)

5 protocols using pelp1 antibody a300 180a

TNBC Cell Lines Characterization

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Human TNBC cell lines: BT-549, HCC-1806, HCC-1937, MDA-MB-231, MDA-MB-453, MDA-MB-468 and SUM-159 were obtained from the American Type Culture Collection (ATCC) and were maintained using ATCC recommended media. All model cells utilized were free of mycoplasma contamination. Additionally, STR DNA profiling was used to confirm the identity of cell lines. The GAPDH, p-ERK (42/44), ERK (42/44), p-S6 (s235/236), S6, p-mTOR (S2448), mTOR, p-4EBP1, 4EBP1 and c-Myc antibodies were obtained from Cell Signaling Technology (Beverly, MA). LAS1L, TEX-10, SENP3 antibodies were purchased from Proteintech (Rosemont, IL). The β-Actin antibody (A-2066), WDR18 antibody (HPA050200) and Vinculin antibody (V9264) were purchased from Millipore Sigma (Burlington, MA). The Ki67 antibody (ab1667) was purchased from Abcam (Cambridge, MA). The PELP1 antibody (A300-180A) was purchased form Bethyl Laboratories Inc. (Montgomery, TX).

Altwegg K.A., Pratap U.P., Liu Z., Liu J., Sanchez J.R., Yang X., Ebrahimi B., Panneerdoss D.M., Li X., Sareddy G.R., Viswanadhapalli S., Rao M.K, & Vadlamudi R.K. (2023). Targeting PELP1 oncogenic signaling in TNBC with the small molecule inhibitor SMIP34. Breast Cancer Research and Treatment, 200(1), 151-162.

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Characterization of Breast Cancer Cell Lines

Cited 2 times

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Human WT- ER⁺ BC cells MCF7, ZR75, and T47D, human mammary epithelial cells (HMEC), murine mammary epithelial cells (HC11), primary human endometrial epithelial cells (HEEC), primary human endometrial stromal cells (HESC), and primary human nontumorigenic immortalized ovarian surface epithelial cells (IOSE-80) were obtained from the American Type Culture Collection (ATCC) and were maintained using ATCC recommended media. MCF7 and ZR75 model cells stably overexpressing PELP1 cDNA or PELP1-shRNA have been described previously (18 (link)). MCF7-TamR (tamoxifen resistant) and MCF7-LTLT (letrozole resistant) cells were cultured in tamoxifen or letrozole (1 μM) containing media (19 ). MCF7^(Y537S) and MCF7^(D538G) MT- ER⁺ BC cell lines were described previously (20 (link)). ZR75^(Y537S) and ZR75^(D538G) MT- ER⁺ BC cell lines were generated in our lab (19 ). All model cells utilized were free of mycoplasma contamination. Additionally, STR DNA profiling was used to confirm the identity of cells. The GAPDH (8884) antibody was obtained from Cell Signaling Technology (Beverly, MA). The β-Actin (A-2066) and Vinculin antibodies (V9264) were purchased from Millipore Sigma (Burlington, MA). The Ki67 antibody (ab1667) was purchased from Abcam (Cambridge, MA). The PELP1 antibody (A300-180A) was purchased form Bethyl Laboratories Inc. (Montgomery, TX).

Altwegg K.A., Viswanadhapalli S., Mann M., Chakravarty D., Krishnan S., Liu Z., Liu J., Pratap U.P., Ebrahimi B., Sanchez J.R., Li X., Ma S., Park B.H., Santhamma B., Chen Y., Lai Z., Raj G.V., Yuan Y., Zhou D., Sareddy G.R., Tekmal R.R., McHardy S., Huang T.H., Rao M.K., Vankayalapati H, & Vadlamudi R.K. (2022). A first-in-class inhibitor of ER coregulator PELP1 targets ER+ breast cancer. Cancer research, 82(20), 3830-3844.

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Cell Line Authentication and Resistance Development

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MCF7, ZR75, and HEK293T cell lines were purchased from the American-Type Culture Collection (ATCC) and maintained in ATCC recommended medium. All model cells utilized were free of mycoplasma contamination and STR DNA profiling of the cells was used to confirm identity. MCF7-TamR cells were cultured in medium supplemented with 1 µmol/L of tamoxifen (H7904, Sigma, St. Louis, MO). MCF7-FR cells were cultured in medium supplemented with 1 µmol/L of fulvestrant (MedChem Express, Monmouth Junction, NJ) for 6 months to develop resistance. The SETDB1 antibody (11231-1-AP) was obtained from Proteintech (Rosemont, IL). The PELP1 antibody (A300-180A) was purchased from Bethyl Laboratories (Montgomery, TX). The ERα (04-820) and β-Actin antibodies (A-2066) were purchased from Millipore Sigma (Burlington, MA). The phospho-ER (Ser167) antibody (PA5-37570) was purchased from Invitrogen (Waltham, MA). The mCherry antibody (632543) was obtained from Takara Bio (San Jose, CA). The GFP antibody (632460) was obtained from Clontech. Antibodies for GST (2624), p-Akt (4060), Akt (9272), tri-methyl lysine motif (K-me3,14680) and GAPDH (8884) were purchased from Cell Signaling Technology (Beverly, MA). For IHC analysis, Ki67 antibody (ab16667) was purchased from Abcam (Cambridge, MA) and p-Akt (05-1003) antibody was purchased from Millipore Sigma.

Liu Z., Liu J., Ebrahimi B., Pratap U.P., He Y., Altwegg K.A., Tang W., Li X., Lai Z., Chen Y., Shen L., Sareddy G.R., Viswanadhapalli S., Tekmal R.R., Rao M.K, & Vadlamudi R.K. (2022). SETDB1 interactions with PELP1 contributes to breast cancer endocrine therapy resistance. Breast Cancer Research : BCR, 24, 26.

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Cell Line Characterization and Antibody Validation

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Human MDA-MB-231 and BT549 cells were purchased from the American-Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in ATCC recommended medium. Cells utilized were free of mycoplasma contamination and identity was confirmed by STR DNA profiling. The PELP1 antibody (A300-180A) was purchased from Bethyl Laboratories (Montgomery, TX, USA). The c-Myc antibody (S1826) was purchased from Clontech (Takara Bio USA, San Jose, CA, USA). The antibody for GAPDH (8884) was purchased from Cell Signaling Technology (Beverly, MA, USA).

Liu Z., Altwegg K.A., Liu J., Weintraub S.T., Chen Y., Lai Z., Sareddy G.R., Viswanadhapalli S, & Vadlamudi R.K. (2022). Global Genomic and Proteomic Analysis Identified Critical Pathways Modulated by Proto-Oncogene PELP1 in TNBC. Cancers, 14(4), 930.

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Estrogen Receptor Signaling Pathway Modulation

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MCF7 and ZR75 cell lines were purchased from the American‐Type Culture Collection (ATCC, Manassas, Virginia) and maintained in RPMI 1640 medium supplemented with 10% FBS (Millipore Sigma, St. Louis, MO, USA). HEK293T cell line was purchased from ATCC and maintained in Dulbecco's Modified Eagle's medium (DMEM) supplemented with 10% FBS. For Fulvestrant (MedChemExpress, Monmouth Junction, NJ, USA) treatment, both MCF7 and ZR75 cells were hormone stripped in phenol red‐free media with 5% dextran‐coated charcoal‐treated FBS (Gemini Bio Products, West Sacramento, CA, USA). After 2 days of incubation, cells were seeded into six‐well plates and treated with Fulvestrant in the presence of 10 nm 17‐β‐estradiol (E2; Sigma). All model cells utilized were free of mycoplasma contamination, and STR DNA profiling of the cells was used to confirm identity. The TFAP2C (sc‐12762) and GFRA1 (sc‐271546) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The PELP1 antibody (A300‐180A) was purchased from Bethyl Laboratories (Montgomery, TX, USA). The ERα (04‐820) antibody was purchased from Sigma. The p‐ERK1/2 (9106), ERK1/2 (9102), p‐Akt (9271), Akt (9272), RET (3223), GST (2624), and GAPDH (8884) antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA). The Ki67 (ab16667) antibody was purchased from Abcam (Cambridge, MA, USA).

Liu J., Liu Z., Li M., Tang W., Pratap U.P., Luo Y., Altwegg K.A., Li X., Zou Y., Zhu H., Sareddy G.R., Viswanadhapalli S, & Vadlamudi R.K. (2021). Interaction of transcription factor AP‐2 gamma with proto‐oncogene PELP1 promotes tumorigenesis by enhancing RET signaling. Molecular Oncology, 15(4), 1146-1161.

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