Anti gh2ax

Manufactured by Merck Group

The Anti-gH2AX is a laboratory equipment product used for the detection and quantification of phosphorylated histone H2AX, which is a marker for DNA double-strand breaks. It is a key tool in the study of DNA damage and repair processes.

Automatically generated - may contain errors

Lab products found in correlation

3 protocols using anti gh2ax

Immunoblotting Procedures and Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blotting was performed as described [19 (link), 20 (link)]. Immunoblotting experiments were carried out according to standard procedures and visualized using the ECL chemiluminescence system (Amersham/ Pharmacia Biotech).
The following antibodies were utilized: anti-CCDC6 (Abcam), anti-USP7 (Bethyl), anti-tubulin, (SIGMA-Aldrich, Inc), anti-PCNA (Millipore), anti-DNMT1 (Cell Signaling), anti-gH2AX (#05636) was from Millipore. Secondary antibodies were from Biorad, California.

Morra F., Merolla F., Criscuolo D., Insabato L., Giannella R., Ilardi G., Cerrato A., Visconti R., Staibano S, & Celetti A. (2019). CCDC6 and USP7 expression levels suggest novel treatment options in high-grade urothelial bladder cancer. Journal of Experimental & Clinical Cancer Research : CR, 38, 90.

+ Open protocol

+ Expand

Immunoblotting for Cellular Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole-cell extracts were prepared according to standard protocols and tested by western blot using anti-GAPDH (Bethyl; dilution: 1:25,000), anti-IGFBP-1 (Santa Cruz; dilution:1:1,000), anti-phospho acetyl-coA-carboxylase (Cell Signaling; dilution: 1:2,000), anti-phospho-AMPK (Cell Signaling; dilution: 1:2,000), anti-phospho-mTOR (Cell Signaling; dilution: 1:2,000), anti-phospho-p53 (Cell Signaling; dilution: 1:2,000), anti-p21 (Santa Cruz; dilution: 1:1,000), anti-gH2Ax (Millipore; dilution: 1:1,000), anti-β-actin (Sigma; dilution: 1:10,000), anti-PKM2 (Santa Cruz; dilution: 1:1,000), anti-PDHK1 (Cell Signaling; dilution: 1:2,000), OxPhos Complex Kit (Anti-Rt/ms; Invitrogen), anti-TFAM (Calbiochem; dilution: 1:1,000) and anti-PGC1α (Abcam; dilution: 1:1,000).

Missios P., Zhou Y., Guachalla L.M., von Figura G., Wegner A., Chakkarappan S.R., Binz T., Gompf A., Hartleben G., Burkhalter M.D., Wulff V., Günes C., Sattler R.W., Song Z., Illig T., Klaus S., Böhm B.O., Wenz T., Hiller K, & Rudolph K.L. (2014). Glucose substitution prolongs maintenance of energy homeostasis and lifespan of telomere dysfunctional mice. Nature Communications, 5, 4924.

+ Open protocol

+ Expand

Chromatin Immunoprecipitation Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

For each ChIP experimental condition (day 0 for pluripotency, day 3 for blood-and neural-differentiation protocols), 3 3 10 6 cells were collected and cross-linked using 1% formaldehyde. Chromatin was fragmented by sonication in buffer containing 1% NP-40 to obtain fragments of 250-500bp in length. Sonicated DNA was subjected to immunoprecipitation using anti-rabbit IgG, anti-gH2A.X, anti-pan-H2A.X (all Millipore) and anti-HA (Thermo Scientific) antibodies conjugated with Dynabeadsâ Protein G (Thermo Fisher Scientific). Immunoprecipitated DNA was further reverse cross-linked, purified and subjected to qPCR/sequencing analyses. For qPCR, sequence enrichment was calculated relative to input signal. Primers used for ChIP-qPCR are listed in Table S1.

Orlando L., Tanasijevic B., Nakanishi M., Reid J.C., García-Rodríguez J.L., Chauhan K.D., Porras D.P., Aslostovar L., Lu J.D., Shapovalova Z., Mitchell R.R., Boyd A.L, & Bhatia M. (2021). Phosphorylation state of the histone variant H2A.X controls human stem and progenitor cell fate decisions. Cell reports, 34(10).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!