The TAGs composition of red sorghum oil was identified using a Waters Xevo‐G2 Q‐TOF MS system combined with the Ultra Performance Convergence Chromatography (UPC2) system (Milford, MA, USA) according to a laboratory protocol (Gao et al., 2017 ).
Xevo g2 q tof ms system
Manufactured by Waters Corporation
Sourced in United States
The Xevo-G2 Q-TOF MS system is a high-resolution mass spectrometer designed for accurate mass analysis and identification of unknown compounds. It utilizes quadrupole and time-of-flight (Q-TOF) technology to provide precise mass measurements and structural information.
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Lab products found in correlation
Upc2 system, by Waters Corporation (1 mentions)
1525 single pump, by Waters Corporation (1 mentions)
Acquity upc2 system, by Waters Corporation (1 mentions)
Masslynx v4, by Waters Corporation (1 mentions)
Waters acquity uplc 1 class system, by Waters Corporation (1 mentions)
Waters acquity uplc beh c18, by Waters Corporation (1 mentions)
4 protocols using xevo g2 q tof ms system
1
Characterization of Red Sorghum Oil
2
Characterization of Seed Oil TAGs
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A Waters Xevo-G2 Q-TOF MS system was utilized to characterize the chemical structures of TAGs in cucumber, tomato, pumpkin, and carrot seed oils. The electrospray ionization (ESI) in positive mode was used in a mass range from 100 to 1200 Da at a capillary voltage of 3.0 kV and a cone voltage of 50.0 V. The source offset was 80.0 V. Leucine enkephalin was used as the lock mass (m/z 556.2771 in ESI+). The source temperature was 120 °C and the desolvation temperature was 500 °C. The desolvation gas (nitrogen) was set at a flow of 800.0 L/h and the collision gas (argon) was set at 150.0 L/h.
Data were collected using Masslynx 4.1 software (Milford, MA, USA) in MSE mode. The MS acquisition method consisted of a low collision energy mode (MS1) and a high collision energy mode (MS2). In the MS1 mode, the collision energy was 6 eV and parent ions were obtained. The information of fragment ions was collected in the MS2 mode and the collision energy was 35 eV. The scan time was 0.2/s.
Data were collected using Masslynx 4.1 software (Milford, MA, USA) in MSE mode. The MS acquisition method consisted of a low collision energy mode (MS1) and a high collision energy mode (MS2). In the MS1 mode, the collision energy was 6 eV and parent ions were obtained. The information of fragment ions was collected in the MS2 mode and the collision energy was 35 eV. The scan time was 0.2/s.
3
Comprehensive TAG Profiling of Berry Seed Oils
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The conditions of UPC2-Q TOF MS were set according to a previously reported laboratory procedure [26 (link),27 (link),28 (link),29 (link)]. Waters Acquity UPC2 system (Milford, MA, USA) equipped with an Acquity UPC2 BEH HSS C18 column (150 mm × 3.0 mm i.d.; 1.7 µm) was utilized for the separation of TAGs. All the other instrument conditions were similar as previous reported method. A Waters 1525 single pump was used as the compensated pump, and pumped 0.3 mL/min of 0.1% ammonium formate in methanol into the MS source. The TAG compositions of the berry seed oils were analyzed and semi-quantified with a Waters Xevo-G2 Q-TOF MS system as previously described. The ion mode, capillary voltage, cone voltage, temperatures of source and desolvation, as well as other parameters were similar as our previous publications. The collision energy was 6 eV in MS1, and information of fragment ions was collected in the MS2 mode and the collision energy was 35 eV, with the scan time set at 0.2/s.
4
Quantitative LC-MS Analysis of Metabolites
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LC-MS analysis was performed on a Waters Acquity UPLC I-Class system (Waters Co., Milford, MA, USA) coupled with a Waters Xevo G2 QTOF MS system (Waters Co.) at the Chuncheon Center of Korea Basic Science Institute (KBSI). Chromatographic separation was performed with a Waters Acquity UPLC BEH C18 (150 mm × 2.1 mm, 1.7 μm) maintained at 40 °C, and the injection volume was set at 2 μL. The mobile phase was composed of two mobile phases (A:0.1 % (v/v) formic acid in distilled water; B: 0.1 % (v/v) formic acid in acetonitrile) with gradient elution (10–90 % B, 0–14 min) at a flow rate of 400 μL/min. QTOF-MS analysis was performed in electrospray ionization (ESI) negative ion mode in a continuum format. MS/MS ion patterns were scanned within the mass range of m/z 100 to 1200, and the collision energy ramp was set from 15 to 45 eV in MSE mode. The ESI source had the following settings: the capillary and cone voltages were 2.5 kV and 45 V, respectively; temperatures of the source and desolvation gas were 120 and 350 °C, respectively; the cone and desolvation gas flows were 50 L/h and 800 L/h, respectively. To ensure reproducibility and accuracy, leucine encephalin was used as the reference compound (m/z 554.2615) at a flow rate of 5 and 200 pg/μL. The instrument was controlled MassLynx V4.1 software (Waters Corporation, Milford, USA).
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