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2 protocols using 2 2 diphenyle 1 picrylhydrazyle

1

Phytochemical Screening and Antioxidant Assays

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Quercetin, morin, rutin, pyrogallol, mandelic acid, hydroxy benzoic acid, phloroglucinol and chlorogenic acid, Follin-ciocalteu reagent, sodium carbonate, 2,2-diphenyle-1 picrylhydrazyle [DPPH], aluminum chloride, sodium nitrate, sodium hydroxide, ethanol, methanol, ascorbic acid, distilled water, 5,5-dithio-bis-nitrobenzoic acid (DTNB), 2,2′-azino-bis-3 ethylbenzothiazoline-6-sulfonic acid (ABTS) were all purchased from Sigma Aldrich. Butyrylthiocholine iodide, acetylthiocholine iodide, acetyl cholinesterase from Electric eel (type-VI-S) and butyrylcholinesterase from Equine were obtained from Sigma-Aldrich USA and were used in enzyme inhibition assays. All solvents used in the assays were of HPLC grades purchased from Dae-Jung Korea.
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2

In Vitro Antioxidant Activity Evaluation

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Alliance Pharmaceuticals and Aries Pharmaceuticals, Peshawar, KPK, Pakistan, donated diclofenac sodium and tramadol. 2,2-Diphenyle-1 Picryl-hydrazyle (DPPH), hydrochloric acid (HCl), Sulphuric Acid (H2SO4) sodium hydroxide (NaOH), magnesium ribbon, petroleum ether, chloroform, n-pentane were purchased from Sigma-Aldrich, Germany. Merck (Darmstadt, Germany) provided methanol, formalin, and acetic acid.
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