Salivary cortisol is a convenient and minimally invasive collection method that provides a valid and reliable measure of the bioactive cortisol in the body.36 To prevent sample contamination from food debris or fluid intake, participants were not allowed to eat or drink 15 minutes before saliva collection. Salivary samples were collected via a cotton mouth swab (Salivette; Sarstedt, Nümbrecht, Germany) at eight 15-minute intervals during each testing sessions; three were collected during baseline (B30, B15, B0) and five samples were collected during recovery (P0, P15, P30, P45, P60). These collection times were deemed consistent with previous research that noted cortisol peaks approximately 20 to 40 minutes after a stressful task begins, before gradually returning to baseline.37 The samples were kept on ice until testing was completed and then centrifuged at 5000 rev/min for 5 minutes, and stored at -80°C. Levels of cortisol were analyzed using an enzyme-linked immunosorbent assay (ELISA; SLV-2930, DRG International, Inc., Hamburg, Germany). The assay was performed according to the manufacturer's directions and read at 450 nm on a luminescence microplate reader (Synergy™ 2 SL; BioTek, Winooski, VT). Analytical sensitivity (lower limit of detection) was 0.14 nmol/L.
Synergy 2 sl
Manufactured by Agilent Technologies
Sourced in United States
The Synergy™ 2 SL is a multi-mode microplate reader from Agilent Technologies. It is designed to perform various detection modes, including absorbance, fluorescence, and luminescence, in a single instrument. The Synergy 2 SL is capable of reading 6- to 384-well microplates and supports a wide range of applications in life science research and drug discovery.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using synergy 2 sl
1
Salivary Cortisol Measurement for Stress Assessment
2
Hsf1-Dependent Stress Response Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The activity of the Hsf1-dependent stress response was determined as described previously43 (link). Briefly, yeast cells were transformed with a plasmid (pAM10) carrying the luciferase NanoLuc under the control of a heat shock promoter (HSE) or with a control plasmid (pAM09). Nano-Glo substrate (Promega) was diluted 1:100 with the supplied lysis buffer and mixed 1:10 with cells grown in SD-Ura medium in a white 96-well plate. Bioluminescence was determined immediately, using a plate reader (Biotek Synergy 2 SL).
3
Mitochondrial ATP Synthesis Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ATP synthesis rate in isolated mitochondria was measured ex-vivo in freshly isolated mitochondria by luciferin-luciferase luminescence assay in a microplate luminometer (Synergy 2 SL, BioTek, Winooski, VT, USA) as previously described [26 (link),28 (link)]. Several combinations of respiratory substrates were contemporarily tested using the following final reaction concentrations (mmol/L): 0.25 pyruvate, 0.0125 palmitoyl-L-carnitine, 2.5 α-ketoglutarate, 0.25 malate (PPKM); 0.025 palmitoyl-L-carnitine, 0.5 malate (PCM); 20 succinate, 0.1 rotenone (SR); 10 glutamate, 5 malate (GM). Measurements obtained were normalized by sample citrate synthase activity, measured spectrophotometrically as referenced [28 (link)].
4
Salivary Cortisol Levels During Physical Work
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Salivary samples were collected using a cotton swab (Salivette; Sarstedt, Nümbrecht, Germany) at baseline (i.e., 07:30) and at the completion of each physical work circuit (i.e., 09:00, 11:15, 13:30, 15:30, 17:30). Further daily samples were taken in both conditions after awakening (i.e., 06:30) and in the evening (i.e., 19:30, 21:30; Fig.1 ). To prevent sample contamination, participants were not allowed to eat or drink 15 min prior to saliva collection. All samples were stored at <−80°C. Samples were then thawed and centrifuged for 10 min at 5000 revolution/min (83 Hz) before assessing salivary cortisol concentration using a high-sensitivity enzyme immunoassay ELISA kit (IBL International, Hamburg, Germany). The assay was performed according to the manufacturer’s directions and read at 450 nm on a luminescence microplate reader (Synergy™ 2 SL, BioTek, Winooski, VT). Analytical sensitivity (lower limit of detection) was 0.14 nmol/L and the intra- and interassay CVs were 7.2% and 10.7% (both mean 13.8 nmol/L), respectively, which are within the acceptable ranges (i.e., accuracy <15%; intra-assay CV <10%; interassay CV <15%; Biopharmaceutics Coordinating Committee, 2001 , Nicolson 2008 ).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!