Z 5000 atomic absorption spectrophotometer

Manufactured by Hitachi

Sourced in Japan

The Z-5000 atomic absorption spectrophotometer is a laboratory instrument manufactured by Hitachi. It is designed to perform quantitative analysis of elements in a sample by measuring the amount of light absorbed by the sample at specific wavelengths. The core function of the Z-5000 is to determine the concentration of elements in a wide range of materials, including liquids, solids, and gases.

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Lab products found in correlation

6400 automatic isoperibol calorimeter, by Parr (1 mentions) Ankom 200 fiber analyzer, by ANKOM Technology (1 mentions) Automatic energy analyzer, by Parr (1 mentions)

Spelling variants of this product

Z-5000 (38 citations) Atomic absorption spectrophotometer (12 citations) Hitachi Z-5000 Absorption Spectrophotometer (8 citations) Z-5000 spectrophotometer (4 citations) Z-5000 polarized Zeeman atomic absorption spectrophotometer (2 citations)

6 protocols using z 5000 atomic absorption spectrophotometer

Analytical Methods for Nutrient Composition

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The nitrogen content was determined by the Kjeldahl method^{22 (link)} and was multiplied by a factor of 6.25 to calculate the protein content.
The glycogen content was determined by the Somogyi method after trichloroacetic
acid extraction, ethanol precipitation, and hydrochloric acid hydrolysis.²³ Taurine was measured as described previously.^{24 (link)} Direct dry ashing was done as described previously.²⁵ The zinc content was determined with Hitachi Z-5000 atomic absorption
spectrophotometer (Tokyo, Japan) at wavelength of 213.8 nm using air-acetylene
flame after direct dry ashing.

Sakaguchi K., Zhong M., Kawai S., Shimizu Y, & Gohda E. (2016). Augmentation of Cytolytic Activity in Murine Natural Killer Cells and Inhibition of Tumor Growth by the Ethanol Fraction of Oyster Extract. Integrative Cancer Therapies, 17(1), 31-40.

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Comprehensive Assessment of Wilson's Disease

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All WD patients and normal controls were tested for urinary copper, serum copper, and liver function. The liver function was graded for Child‐Pugh scoring (Table 1). The metal index was determined by atomic absorption spectrophotometer flame method. The measuring instrument was Hitachi Z‐5000 atomic absorption spectrophotometer. Hollow CATHODE lamp: Copper L233 wavelength: 324.9 nm, current: 10 mA.
The modified Young scale (Zhou et al., 2011 ) (including eight categories of language, throat muscle tone, limb muscle tone, ataxia, tremor, dance‐like movement, gait, and advanced neural activity) was used to score WD patients for neurological symptoms. According to the clinical symptoms of cerebral type WD patients and the scores of modified Young scale, cerebral type WD patients can be divided into the following four types: (1) involuntary movements; (2) Parkinson's symptoms; (3) oral‐mandibular dystonia; (4) mental disorders.
Neurological symptom score, metal metabolism, liver function, and imaging data of WD patients were collected. The use of metal chelating agent in WD patients in drug withdrawal group and the reasons for stopping metal chelating agent were collected.

Zhou X., Liao J., Liu Y., Qin H, & Xiao X. (2023). Symptom aggravation after withdrawal of metal chelating agent therapy in patients with Wilson's disease. Brain and Behavior, 13(9), e3170.

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Cadmium Quantification in Submandibular Glands

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Known-weight halves of the left submandibular glands were wet-digested with a trace-pure concentrated nitric acid using UniClever II microwave system (Plazmatronika, Wroclaw, Poland) and then the wet-digests were diluted with ultra-pure water. The concentration of cadmium in such preparations was determined by the graphite furnace AAS method (GF AAS) using HITACHI Z-5000 atomic absorption spectrophotometer (Tokio, Japan) equipped with a graphite cuvette (Pyro cuvette A, Hitachi) and a hollow cathode lamp for this element assay (Photron, Narre Waren, Australia). The concentration of cadmium measured in the simultaneously analysed reference material (0.0020 ± 0.0001; Bovine muscle, ERM-BB184) was consistent with the value provided by the producer (0.0022 μg/g; uncertainty 0.0004 μg/g). The CV was < 6%.

Dąbrowski A., Onopiuk B.M., Car H., Onopiuk P., Dąbrowska Z.N., Rogalska J., Brzóska M.M, & Dąbrowska E. (2020). Beneficial Impact of an Extract from the Berries of Aronia melanocarpa L. on the Oxidative-Reductive Status of the Submandibular Gland of Rats Exposed to Cadmium. Antioxidants, 9(2), 185.

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Nutrient Digestibility Analysis in Fecal Samples

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The fecal samples were defrosted at 4 °C and subsequently dried at 65 °C within an oven for 72 h. The feed and fecal samples were crushed and sieved through 40 mesh. Dry matter (DM), ash, crude protein (CP) ether extract (EE), calcium (Ca) and phosphorus (P) were determined based on the methods of the Association of Official Analytical Chemists (AOAC) [15 ], respectively. Organic matter (OM) was calculated (OM (%) = 1-Ash (DM-basis) × 100%). Neutral detergent fiber (NDF) and acid detergent fiber (ADF) were determined with reference to the method of van Soest et al. [16 (link)] using a filter bag and ANKOM200 fiber analyzer (Ankom, USA). Gross energy (GE) was analyzed using a 6400 automatic isoperibol calorimeter (Parr, USA). Chromium (Cr) level was determined by Z-5000 atomic absorption spectrophotometer (Hitachi, Japan), according to the methodology of Williams et al. [17 (link)], to calculate the apparent total tract digestibility (ATTD) of nutrients with the following equation:

Ma J., Ma H., Liu S., Wang J., Wang H., Zang J., Long S, & Piao X. (2022). Effect of Mulberry Leaf Powder of Varying Levels on Growth Performance, Immuno-Antioxidant Status, Meat Quality and Intestinal Health in Finishing Pigs. Antioxidants, 11(11), 2243.

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Biochemical Analysis of Duckling Organs

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When the ducklings were 3 weeks old, 12 ducklings were randomly chosen from each group and sacrificed by cervical dislocation. The heart, gizzard, liver, kidney, breast, leg, and tibias (left and right) of ducklings were sampled and weighed. The relative weights of organs and carcass were calculated as follows:
Blood samples were collected into heparinized tubes by cardiac puncture. Plasma was obtained after the removal of red blood cells by centrifugation at 1500× g for 15 min. All plasma samples were stored at −20 °C until analysis. The concentrations of blood urea nitrogen (BUN), uric acid (UA), creatinine (CREA), creatine phosphokinase (CPK), glutamate oxaloacetate transaminase (GOT), glutamate pyruvic transaminase (GPT), alkaline phosphatase (ALK), cholinesterase (CHE), total protein (TP), albumin (ALB), and globulin (GLO) in plasma were determined using an automated clinical chemistry analyzer (Automatic Analyzer 7150, Hitachi, Tokyo, Japan), according to the manufacturer’s instructions. The levels of calcium (Ca) and magnesium (Mg) in plasma were determined with flame atomic absorption spectroscopy (Atomic Absorption Spectrophotometer Z-5000, Hitachi, Tokyo, Japan).

Tso K.H., Lumsangkul C., Cheng M.C., Ju J.C., Fan Y.K, & Chiang H.I. (2021). Differential Effects of Green Tea Powders on the Protection of Brown Tsaiya and Kaiya Ducklings against Trichothecene T-2 Toxin Toxicity. Animals : an Open Access Journal from MDPI, 11(9), 2541.

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Digestibility Analysis of Fecal Samples

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From d 25 to d 28, about 100 g of feces were collected and stored at −20 °C before oven drying. The samples were dried at 65 °C for 72 h, and then were ground and passed through a 1 mm screen (40 mesh) before analysis.
Feed and fecal samples were analyzed in terms of crude protein (CP), gross energy (GE), ether extract (EE), organic matter (OM), Ca, P, and dry matter (DM) with an automatic isoperibol oxygen bomb calorimeter (Parr 1281, Automatic Energy Analyzer; Moline, IL, USA). Digestibility was calculated by measuring chromium content in the diets and feces (Atomic Absorption Spectrophotometer; Z-5000, Hitachi, Tokyo, Japan).

Shang L., Zhou J., Tu J., Zeng X, & Qiao S. (2022). Evaluation of Effectiveness and Safety of Microcin C7 in Weaned Piglets. Animals : an Open Access Journal from MDPI, 12(23), 3267.

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