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Abt128

Manufactured by Merck Group
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The ABT128 is a piece of laboratory equipment manufactured by Merck Group. It is designed for conducting various scientific experiments and analyses. The core function of the ABT128 is to provide a controlled and precise environment for performing a range of laboratory procedures.

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Lab products found in correlation

Mini protean tgx gel, by Bio-Rad (2 mentions) Nb300 221, by Novus Biologicals (2 mentions) Sc 47734, by Santa Cruz Biotechnology (2 mentions) Pvdf membrane, by Bio-Rad (2 mentions) Ab14106, by Abcam (1 mentions) Ab50011, by Abcam (1 mentions) Ab52866, by Abcam (1 mentions) Ab47363, by Abcam (1 mentions) Ab7952, by Abcam (1 mentions) Ab189403, by Abcam (1 mentions) Ab17942, by Abcam (1 mentions) Ab46794, by Abcam (1 mentions) Ab134175, by Abcam (1 mentions) Ab5694, by Abcam (1 mentions) Ab32072, by Abcam (1 mentions) Ab4821, by Abcam (1 mentions)

4 protocols using abt128

1

Protein Expression Analysis in Cells

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The content of PC1, SM22, ACTA2, CNN1, p38, ERK, JNK, PI3K and Myc, CyclinD1, and the phosphorylation of p38, ERK, JNK and PI3K were determined by Western blot. Preparation of whole cell lysates and tissue homogenates, and the immunoblotting assay were performed according to previously described procedures.18 (link) The primary antibodies were obtained from the following sources: anti-Polycystin-1 (ABT128; Millipore); anti-SM22 alpha (ab14106; Abcam, Cambridge, UK); anti-alpha smooth muscle Actin (ab5694; Abcam); anti-Calponin (ab46794; Abcam); anti-alpha Tubulin (ab52866; Abcam); anti-p38 (phospho Y182) (ab47363; Abcam); Anti-p38 (ab7952; Abcam); Anti-ERK1 (pT202/pY204) + ERK2 (pT185/pY187) (ab50011; Abcam); Anti-ERK1 + ERK2(ab17942; Abcam); Anti-JNK1+JNK2 (phospho T183 + Y185) antibody (ab4821); Anti-JNK1+JNK2 (ab37228; Abcam); Anti-PI3K p85 (phospho Y607); Anti-PI3K p85 (ab189403; Abcam); anti-myc (ab32072; Abcam) and anti-Cyclin D1 (ab134175; Abcam).
Feng J., Ge S., Zhang L., Che H, & Liang C. (2016). Aortic Dissection is Associated with Reduced Polycystin-1 Expression, an Abnormality That Leads to Increased ERK Phosphorylation in Vascular Smooth Muscle Cells. European Journal of Histochemistry : EJH, 60(4), 2711.
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2

Aortic Remodeling via PC1 Immunohistochemistry

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The thoracic aorta were fixed in 4% paraformaldehyde in PBS, embedded in paraffin, sectioned into 5-μm thick slices, blocked with BSA, the sections were incubated with primary antibody against PC1 (ABT128, Millipore, Billerica, MA, USA) overnight at 4°C and then biotinylated secondary antibody for 60 min at room temperature. Vascular remodeling was analyzed under light microscopy (BX 45, Olympus Corporation, Tokyo, Japan).
Feng J., Ge S., Zhang L., Che H, & Liang C. (2016). Aortic Dissection is Associated with Reduced Polycystin-1 Expression, an Abnormality That Leads to Increased ERK Phosphorylation in Vascular Smooth Muscle Cells. European Journal of Histochemistry : EJH, 60(4), 2711.
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3

Western Blot Analysis of Polycystins

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Cells were lysed in RIPA buffer boiled at 95 °C for 5 min. And after quantification loaded to 4-15% mini-Protean TGX gel (4568086, Bio-Rad) and transferred to PVDF membranes (162-0177, Bio-Rad). The membranes were blotted for polycystin-1, rabbit polyclonal antibody (1:2,000, ABT128, Millipore) or polycystin-2, mouse monoclonal antibody (1:1,000, sc-47734, Santa Cruz Biotechnology). GAPDH was used for loading control, mouse monoclonal antibody (1:2,000, NB300-221, NovusBio). For full details see Supplementary Methods.
Xu Y., Kuppe C., Perales-Patón J., Hayat S., Kranz J., Abdallah A.T., Nagai J., Li Z., Peisker F., Saritas T., Halder M., Menzel S., Hoeft K., Kenter A., Kim H., van Roeyen C.R., Lehrke M., Moellmann J., Speer T., Buhl E.M., Hoogenboezem R., Boor P., Jansen J., Knopp C., Kurth I., Smeets B., Bindels E., Reinders M.E., Baan C., Gribnau J., Hoorn E.J., Steffens J., Huber T.B., Costa I., Floege J., Schneider R.K., Saez-Rodriguez J., Freedman B.S, & Kramann R. (2022). Adult human kidney organoids originate from CD24+ cells and represent an advanced model for adult polycystic kidney disease. Nature genetics, 54(11), 1690-1701.
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4

Western Blot Analysis of Polycystins

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Cells were lysed in RIPA buffer boiled at 95 °C for 5 min. And after quantification loaded to 4-15% mini-Protean TGX gel (4568086, Bio-Rad) and transferred to PVDF membranes (162-0177, Bio-Rad). The membranes were blotted for polycystin-1, rabbit polyclonal antibody (1:2,000, ABT128, Millipore) or polycystin-2, mouse monoclonal antibody (1:1,000, sc-47734, Santa Cruz Biotechnology). GAPDH was used for loading control, mouse monoclonal antibody (1:2,000, NB300-221, NovusBio). For full details see Supplementary Methods.
Xu Y., Kuppe C., Perales-Patón J., Hayat S., Kranz J., Abdallah A.T., Nagai J., Li Z., Peisker F., Saritas T., Halder M., Menzel S., Hoeft K., Kenter A., Kim H., van Roeyen C.R., Lehrke M., Moellmann J., Speer T., Buhl E.M., Hoogenboezem R., Boor P., Jansen J., Knopp C., Kurth I., Smeets B., Bindels E., Reinders M.E., Baan C., Gribnau J., Hoorn E.J., Steffens J., Huber T.B., Costa I., Floege J., Schneider R.K., Saez-Rodriguez J., Freedman B.S, & Kramann R. (2022). Adult human kidney organoids originate from CD24+ cells and represent an advanced model for adult polycystic kidney disease. Nature genetics, 54(11), 1690-1701.
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