For scanning electron microscopy, crosslinked scaffolds were washed in demineralized water to remove storage medium, and subsequently frozen at −80 °C. The frozen scaffolds were lyophilized, mounted on stubs with double-sided carbon tape, and coated with an ultrathin gold layer (Scancoat Six Sputter Coater, Edwards, Crawley, United Kingdom). The samples were evaluated with a Sigma 300 field emission scanning electron microscope (Carl Zeiss B. V., Sliedrecht, The Netherlands) with an accelerating voltage of 10 kV. The pore sizes of the different scaffolds were determined using ImageJ analysis software.
Scancoat six sputter coater
Manufactured by Edwards Lifesciences
Sourced in United Kingdom
The Scancoat Six Sputter Coater is a laboratory equipment designed for the deposition of thin films on various substrates. It is used to apply conductive coatings to samples in preparation for analysis using electron microscopy techniques.
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Lab products found in correlation
3 protocols using scancoat six sputter coater
1
Characterizing Crosslinked Scaffold Pores
2
SEM Morphological Analysis of Collagen Plugs
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Scanning electron microscopy (SEM) was used to analyze the morphology and structure of the collagen plugs. After lyophilization, specimens were fixed on a stub with double-sided carbon tape, sputtered with gold for 60 s (Scancoat Six Sputter Coater, Edwards, Crawley, United Kingdom) and examined with a Zeiss Sigma 300 Field Emission Scanning Electron microscope (Zeiss, Jena, Germany) at an accelerating voltage of 5 kV.
3
SEM Imaging of Material Surfaces
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Scanning electron microscopy (SEM) images of the structures surfaces were taken using a Karl-Zeiss EVO series instrument. All samples were sputtered with a gold coating (~8 nm) using an Edwards Scancoat Six Sputter Coater prior to analysis under SEM. During sputtering, samples were immobilised onto glass substrates using the silicone elastomer to ensure they remained stable.
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