Lung CICs were dissociated into single-cell suspensions by Accumaxtm cell counting solution (EMD Millipore) and stained with mouse anti-CD133-PE antibody (clone AC133, Miltenyi Biotec) and mouse anti-CD44-FITC antibody (clone DB105, Miltenyi Biotec). The cells were sorted by flow cytometry. Apoptosis was measured by flow cytometry analysis of PE-labeled Annexin-V (BD Biosciences) and 7-AAD (BD Biosciences) co-staining, according to the manufacturer’s protocol. ALDH assay was performed by flow cytometry analysis with ALDEFLUOR™ Kit (STEMCELL), following the recommendation of the manufacturer.
Mouse anti cd133 pe antibody clone ac133
Manufactured by Miltenyi Biotec
The Mouse anti-CD133-PE antibody (clone AC133) is a flow cytometry reagent used for the detection and identification of CD133-positive cells. CD133 is a transmembrane glycoprotein that has been widely used as a marker for various stem and progenitor cell populations. This antibody conjugated with the fluorescent dye Phycoerythrin (PE) enables the visualization and analysis of CD133-expressing cells by flow cytometry.
Automatically generated - may contain errors
2 protocols using mouse anti cd133 pe antibody clone ac133
1
Lung CICs Isolation and Characterization
2
Isolation and Analysis of Lung TICs
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Lung TICs spheres were dissociated into single-cell suspensions by StemPro Accutase (ThermoFisher Scientific, USA). The cells were stained with mouse anti-CD133-PE antibody (clone AC133; Miltenyi Biotec) and mouse. The cells were sorted by flowcytometry. The cells were centrifuged and the pellet was used to extract RNA and to prepare cell lysate for western blot analyses to examine G9A expression.
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