H23 crl 5800

The non-small cell lung cancer (NSCLC) A549 (CCL-185) and H23 (CRL-5800) cell lines were purchased from ATCC (Middlesex, UK). Copper chloride (CuCl₂, Cu), disulfiram (DS), diethyldithiocarbamate (DDC), S-methyl-N,N-diethyldithiocarbamate (S-Me-DDC), tetramethylthiuram disulfide (TMDS), 2-hydroxy-dithiobenzoic acid (HDTA), 4-imidazoledithiocarboxylic acid (IDTA), 2,4,6-Trimercaptotriazine (TMT) and poly-2-hydroxyethyl methacrylate (poly-HEMA) were purchased from Sigma (Dorset, UK). Bis(N,N-diethyldithiocarbamate)-copper(II) (Cu-DDC) was from Santa Cruz (Dallas, TX, USA). Annexin V kit was from Roche Applied Sciences (Burgess Hill, UK). Fc OxyBURST Assay Reagents was purchased from Invitrogen, Molecular Probes (Waltham, MA, USA). ALDEFLUOR kit was from StemCell Tech (Durham, NC, USA). FITC mouse Anti-Human CD44 from BD Biosciences (Oxford, UK).

Human lung cancer H460 (HTB-177), H23 (CRL-5800) and A549 (CCL-185) cells were obtained from ATCC (Rockville, MD, USA). H460 and H23 cells were cultured in RPMI-1640, and A549 cells were cultured in DMEM. All the cell cultures were supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine and 100 units/mL penicillin/streptomycin and incubated in 5% CO₂ at 37 °C. The media and supplements were obtained from Thermo Fisher Scientific (Waltham, MA, USA).