Tumors treated with PBS, d106S, or d106S-IL12 were collected on day 10 and day 17 following one or four intratumoral injections, respectively, were snap frozen in liquid nitrogen. Frozen tumors were ground into a powder and tissue homogenate generated by addition of ice-cold RIPA lysis buffer (150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 25 mM Tris) and 1:100 protease/phosphatase inhibitor cocktail (ThermoFisher). Homogenate was incubated by shaking for 30 min at 4°C and subsequently centrifuged at 14,000 × g for 15 min to yield clarified lysate. The clarified supernatant was subjected to a 32-plex cytokine/chemokine array (Eve Technologies). Expression of IL-13 was undetectable in all samples and is absent from the heatmap.
32 plex cytokine chemokine array
Manufactured by Eve Technologies
The 32-plex cytokine/chemokine array is a multiplex assay that allows for the simultaneous detection and quantification of 32 different cytokines and chemokines from a single sample. This lab equipment provides a comprehensive analysis of the immune and inflammatory status of a sample.
Automatically generated - may contain errors
4 protocols using 32 plex cytokine chemokine array
1
Tumors Analyzed for Cytokine Profiles
2
Cytokine profiling of B16Nectin1 tumors
3
Cytokine profiling of B16Nectin1 tumors
Check if the same lab product or an alternative is used in the 5 most similar protocols
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B16Nectin1 tumors collected on day 10 and day 17 following one or four intratumoral injections, respectively, were snap frozen in liquid nitrogen. Frozen tumors were ground into a powder and tissue homogenate generated by addition of ice-cold RIPA lysis buffer (150mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 25mM Tris) and 1:100 protease/phosphatase inhibitor cocktail (ThermoFisher). Homogenate was incubated shaking for 30 min at 4◦C and subsequently centrifuged at 14,000 x g for 15 min to yield clarified lysate. The clarified supernatant was subjected to a 32-plex cytokine/chemokine array (Eve Technologies). The protein concentrate of lysate was determined using the Micro BCA Protein Assay Kit (ThermoFisher) to normalize samples to contain an equal amount of protein.
4
Tumors Analyzed for Cytokine Profiles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tumors treated with PBS, d106S, or d106S-IL12 were collected on day 10 and day 17 following one or four intratumoral injections, respectively, were snap frozen in liquid nitrogen. Frozen tumors were ground into a powder and tissue homogenate generated by addition of ice-cold RIPA lysis buffer (150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 25 mM Tris) and 1:100 protease/phosphatase inhibitor cocktail (ThermoFisher). Homogenate was incubated by shaking for 30 min at 4°C and subsequently centrifuged at 14,000 × g for 15 min to yield clarified lysate. The clarified supernatant was subjected to a 32-plex cytokine/chemokine array (Eve Technologies). Expression of IL-13 was undetectable in all samples and is absent from the heatmap.
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