Mk 0752

Manufactured by MedChemExpress

Sourced in United States

MK-0752 is a chemical compound used in laboratory research. It is a potent and selective inhibitor of the enzyme 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1). 11β-HSD1 plays a role in the regulation of glucocorticoid levels within cells. The primary function of MK-0752 is to serve as a research tool for studying the effects of 11β-HSD1 inhibition in various experimental models.

Automatically generated - may contain errors

Lab products found in correlation

Vardenafil, by Merck Group (1 mentions) Rat tail collagen type 1, by BD (1 mentions) Blue loading buffer, by Cell Signaling Technology (1 mentions) Oridonin, by Merck Group (1 mentions) Milciclib, by MedChemExpress (1 mentions) Vascular smooth muscle cell growth kit, by American Type Culture Collection (1 mentions) Sk ut 1b, by American Type Culture Collection (1 mentions) Hut smc, by American Type Culture Collection (1 mentions) Htb 88, by American Type Culture Collection (1 mentions) Sk lms 1, by American Type Culture Collection (1 mentions)

2 protocols using mk 0752

High-Throughput Compound Screening for Drug Discovery

Check if the same lab product or an alternative is used in the 5 most similar protocols

Two collections of compounds assembled at the NCATS were screened A collection of 44,420 diverse small molecule drugs; and the Mechanism Interrogation PlatE 4.0 collection of 386 pharmacologically active small molecules (22 (link)). The active compounds were re-purchased for repeat and secondary assays. Milciclib (catalog #PHA-848125), MK-0752 (#HY-10974) and PP-121 (#HY-10372) were purchased from MedChem Express. Ouabain (#1076) and AMG-51 (#SYN-111) were purchased from Fisher Scientific. Vardenafil (#Y0001647) and Oridonin (#O9639) were purchased from Sigma-Aldrich. Peruvoside (# P227570) was purchased from Toronto Research Chemicals. NCGC00117362 (#D233–0871), NCGC00111761 (#C686–0165), NCGC00117328 (#D233–0834), NCGC00117505 (#D244–0327), NCGC00117477 (#D244–0252), NCGC00117166 (#D233–0497), NCGC00115018 (#D053–0260), NGC00117330 (#D233–0835) and NCGC00117364 (#D233–0885) were purchased from ChemDiv. Collagen type I (rat-tail), and fibronectin (human) were purchased from BD Biosciences. Rabbit anti-phospho m-TOR against Ser²⁴⁴⁸ (#2971), rabbit anti-mTOR (#2972), rabbit anti-phospho Rb against Ser^807/811 (#8516, clone D20B12), mouse anti-Rb (#9309), mouse anti-Cdk6 (#3136), lysis buffer (#9803), and 3x Blue Loading Buffer (#56036) were purchased from Cell Signaling Technology. Mouse anti-Cdk1/Cdk2 (#sc-53219) was purchased from Santa Cruz Biotechnology Inc.

Kenny H.A., Lal-Nag M., Shen M., Kara B., Nahotko D.A., Wroblewski K., Fazal S., Chen S., Chiang C.Y., Chen Y.J., Brimacombe K.R., Marugan J., Ferrer M, & Lengyel E. (2019). Quantitative high-throughput screening using an organotypic model identifies compounds that inhibit ovarian cancer metastasis. Molecular cancer therapeutics, 19(1), 52-62.

+ Open protocol

+ Expand

Inhibition of γ-secretase in uLMS Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Two human uLMS cancer cell lines, SK-UT-1B (ATCC HTB-115™) and SK-LMS-1 (ATCC HTB-88™), and one human uterine smooth muscle cell line HUt-SMC (ATCC PCS-460-011™), were purchased from ATCC (Manassas, VA, USA). uLMS cell lines were cultured in Eagle’s Minimum Essential Medium (EMEM) (ATCC) with 10% Fetal Bovine Serum (FBS) and 1% Pen-Strep. HUt-SMC was cultured in Vascular Smooth Muscle Cell Growth Kit (ATCC) as per the manufacturer’s instructions. All cells were maintained in a humidified incubator under standard culture conditions of 21% O₂ and 5% CO₂ at 37 °C.
γ-secretase activity was blocked by DAPT (Sigma-Aldrich, St. Louis, MO, USA) or MK-0752 (MedChemExpress, Monmouth, NJ, USA) dissolved in DMSO. SK-UT-1B and SK-LMS-1 cells were grown to greater than 80% confluence in 60 mm plates or 6-well plates and treated with DAPT or MK-0752. Vehicle control (DMSO) was used at equal volume to treatment.

Abedin Y., Gabrilovich S., Alpert E., Rego E., Begum S., Zhao Q., Heller D., Einstein M.H, & Douglas N.C. (2022). Gamma Secretase Inhibitors as Potential Therapeutic Targets for Notch Signaling in Uterine Leiomyosarcoma. International Journal of Molecular Sciences, 23(11), 5980.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!