Mass spectrometric detection was carried out on a Solarix 7.0T FT-ICR mass spectrometer (Bruker, Bremen, Germany) coupled with an electrospray ionization (ESI) interface. The full-scan MS data acquired in both positive and negative modes scanned from 100 to 1200 Da. The capillary voltage was 4500 V, the endplate offset was 500 V, dry gas flow was 8 L/min, dry gas temperature was 200 °C, and nebulizer gas pressure was 4 bar. The collision gas and nebulizing gas were high-purity argon (Ar) and high-purity nitrogen (N2), respectively. Full-scan MS data were acquired over an m/z range of 100–1200 Da. And the collision energy were set from 10 to 40 eV for fragmentation information. Bruker Compass Hystar (version 4.1, Bruker Daltonics, Bremen, Germany) and Fourier Transform Mass Spectrometer Control (version 2.1, Bruker Daltonics, Bremen, Germany) were used for instrument control and data acquisition. Data Analysis Software (version 4.4, Bruker Daltonics, Bremen, Germany) was used for data analysis.
Compass hystar
Manufactured by Bruker
Sourced in Germany
The Compass Hystar is a high-performance liquid chromatography (HPLC) system designed for a wide range of analytical applications. It provides precise and reliable separation, detection, and quantification of analytes in complex samples.
Automatically generated - may contain errors
Lab products found in correlation
Data analysis software, by Bruker (1 mentions)
Solarix 7.0t ft icr ms system, by Bruker (1 mentions)
Shim pack xr ods c18 column, by Shimadzu (1 mentions)
Tune mix, by Agilent Technologies (1 mentions)
Microtof q, by Bruker (1 mentions)
Compact qtof, by Bruker (1 mentions)
Zorbax eclipse plus column, by Agilent Technologies (1 mentions)
Maxis q tof, by Bruker (1 mentions)
Dataanalysis, by Bruker (1 mentions)
Compass dataanalysis, by Bruker (1 mentions)
Microtofcontrol, by Bruker (1 mentions)
Cbm 20a controller, by Shimadzu (1 mentions)
8 protocols using compass hystar
1
Mass Spectrometric Analysis of Compounds
2
Metabolic Profiling by UHPLC-FT-ICR MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All analysis were performed on a UHPLC-FT-ICR MS instrument, an Agilent 1260 system coupled with a Bruker Solarix 7.0 T FT-ICR MS system (Bruker, Germany) equipped with an electrospray ionization source (ESI). Chromatographic separation was performed on a Shim-pack XR-ODS C18 column (75 mm × 3.0 mm, 2.2 μm) (SHIMADZU, Japan), the column temperature was maintained at 35 °C, the flow rate was set at 0.50 ml min−1. The mobile phase, consisting of 0.1% formic acid water (A) and acetonitrile (B), was delivered using a liner gradient program as follows: 10–20% (B) in 0–13 min, 20–35% (B) in 13–18 min, 35–50% (B) in 18–20 min, 50–50% (B) in 20–30 min. The injection volume was 5 μl.
For MS detection, the instrument was operated in negative ion mode, and full-scan mass rage was 100–1000 Da. The optimal conditions were as follows: a nebulizer gas pressure of 4.0 bar, a dry gas flow rate of 8.0 L min−1, a capillary voltage of −3.5 kV, an end plate off set of −500 V, and a transfer capillary temperature of 250 °C. While in MS/MS experiments, the collision energy was initially set at 20 V of the preferred ions and then adjusted according to the fragments. FT MS control and Bruker Compass-Hystar (Bruker, Germany) were used to control the equipment and for data acquisition and analysis, respectively.
For MS detection, the instrument was operated in negative ion mode, and full-scan mass rage was 100–1000 Da. The optimal conditions were as follows: a nebulizer gas pressure of 4.0 bar, a dry gas flow rate of 8.0 L min−1, a capillary voltage of −3.5 kV, an end plate off set of −500 V, and a transfer capillary temperature of 250 °C. While in MS/MS experiments, the collision energy was initially set at 20 V of the preferred ions and then adjusted according to the fragments. FT MS control and Bruker Compass-Hystar (Bruker, Germany) were used to control the equipment and for data acquisition and analysis, respectively.
3
Electrospray Ionization Mass Spectrometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The spectra were recorded on electrospray quadrupole/time-of-flight-type mass spectrometer MicrOTOF-Q (Bruker Daltonics, Germany) operated in positive ion mode. The performance and resolution were verified using Tunemix (Agilent Technologies, USA) with resolution at m/z 1,222 of 12,000. Mass calibration was achieved using Tunemix (internal calibration) between m/z 50 and 3,000 in the same acquisition mode that affords accuracy of 10 p.p.m. One acquisition consisted of infusing the sample at 50 μM in CH3CN:H2O (1:1) during 2 min, followed by 1-min infusion of Tunemix. The capillary voltage was set to 4,500 V and the ion energy at 5 eV. The system was controlled by Compass Hystar (Bruker Daltonics, Germany). Before each sample injection, a blank consisting of CH3CN:H2O (1:1) was injected. Data analysis was performed with the software Data Analysis 4.
4
HPLC-MS analysis of complex samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HPLC analysis was performed on a VWR Hitachi LaChrom Elite machine, equipped with a L-2130 pump, L-2200 autosampler, and L-2455 DAD detector. Samples were run on a ZORBAX Eclipse Plus column (C18, 4.6 × 100 mm, 3.5 µm; Agilent) at a flow rate of 1 mL min−1, using 0.1% formic acid in water (A) and acetonitrile (B) as mobile phase (gradient: 50–70% B from 0–5 min, 70–92% B from 5–10 min, 92–96% B from 10–28 min). For HRESI-MS characterisation, the HPLC system was coupled to a Bruker Compact QToF mass spectrometer (Bruker Daltonics, Bremen, Germany), operated via the Compass HyStar control platform (Bruker Daltonics). Measurements were performed in negative ionisation mode at 30,000 m Δm−1 resolving power, recording ions in the full scan range of m/z 50–1300. The m/z values obtained were recalibrated using the expected cluster ion m/z values of a sodium formate-isopropanol solution, which was injected at the beginning of each run. For LC-MS/MS analysis, the multiple reaction monitoring (MRM) mode of the instrument was used, setting the collision energy to 30, 35, and 40 eV.
5
Q-ToF Mass Spectrometry Protocol for Synthetic Mixtures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MS data were collected using a Bruker MaXis Q-ToF with Compass Hystar. For positive and negative ionization, the following settings were used: capillary voltage of 4,500 V; nebulizer gas pressure of 2 bar; ion source temperature of 200 °C; and dry gas flow of 9 l min–1. All spectra were collected using data-dependent acquisition with a scan range of 100–2,000 m/z, for which the spectral rate was set to 3 Hz and 10 Hz for MS1 and MS2, respectively. The MS/MS collision energies that were used are available in Supplementary Table 4 . The five most intense ions per MS1 were selected for MS/MS acquisition, and an active exclusion was enabled and set to release after 30 s and only allow 2 spectra, after which the precursor ions were reconsidered only if the current intensity/previous intensity was >2. Hexakis(1H,1H,2H-perfluoroethoxy)phosphazene (CAS 186817-57-2) was used as an internal calibrant, and lock mass correction was applied on raw.d files in Bruker DataAnalysis. LC–MS/MS data for the synthetic mixtures from the Q-ToF are publicly available and deposited into the MassIVE data repository under accession numbers MSV000089491 (acyl amides), MSV000089493 (acyl esters) and MSV000087522 (conjugated bile acids).
6
Mass Spectrometry Data Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MS data was collected using Bruker Compass/Hystar and processed using Bruker QuantAnalysis software. Statistics (t-test, ANOVA, variances, standard deviation) were calculated using GraphPad Prism and Microsoft Excel.
7
Metabolite Identification by FT-MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Metabolites were identified and analyzed by FT-MS control, Bruker Compass-Hystar and Data Analysis Software (Bruker, Germany).
8
HILIC-MS Analysis of Metabolites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The HPLC setup was based on a Shimadzu system and consisted of a CBM-20A controller, a SIL-HTA auto sampler, two 10ADVP pumps, a DGU-14A degasser, a CTO-10AVP column oven and an SPD-10AVP variable wavelength detector. The setup was coupled to a micrOTOF time of flight mass analyzer (Bruker) and controlled by Compass HyStar (Bruker) version 3.2 and microTOFControl (Bruker) version 3.0. Measurement data was analyzed using Compass DataAnalysis version 5.0 R1 (Bruker).
The column oven was set to 40 °C. A SeQunant ZIC-HILIC (Merk KGaA, 150 × 2.1 mm, particle size 3.5 µM, pore size 200 Å) with a SeQunant ZIC-HILIC (Merk KGaA, 20 × 2.1 mm, particle size 5 µM, pore size 200 Å) precolumn was used as stationary phase. The mobile phase was a mixture of water (0.1 % NH4FA, pH 3.2) and acetonitrile. The injection volume was 5 µL. The following gradient was used with a flow rate of 150 µL/min (Table 2 ).
The column oven was set to 40 °C. A SeQunant ZIC-HILIC (Merk KGaA, 150 × 2.1 mm, particle size 3.5 µM, pore size 200 Å) with a SeQunant ZIC-HILIC (Merk KGaA, 20 × 2.1 mm, particle size 5 µM, pore size 200 Å) precolumn was used as stationary phase. The mobile phase was a mixture of water (0.1 % NH4FA, pH 3.2) and acetonitrile. The injection volume was 5 µL. The following gradient was used with a flow rate of 150 µL/min (
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!