A549 cells (5 × 106) were suspend in cell staining buffer (BioLegend, California, USA). Firstly, non-specific binding was blocked using Human TruStain FcX™ (5 µl per million cells in 100 µl staining volume, BioLegend) for 15 min. Then the FITC anti-human CD324 (E-Cadherin) antibody (BioLegend) and APC anti-human CD325 (N-Cadherin) antibody (BioLegend) were incubated on ice for 20 min in the dark. After washing with cell staining buffer, flow cytometry was performed to select the target cells. Isotype controls were included in all flow cytometry experiments.
Fitc anti human cd324 e cadherin antibody
Manufactured by BioLegend
Sourced in United States
The FITC anti-human CD324 (E-Cadherin) antibody is a laboratory reagent used to detect the presence of CD324, also known as E-Cadherin, on the surface of human cells. It is conjugated with the fluorescent dye FITC (Fluorescein isothiocyanate) for visualization and analysis purposes.
Automatically generated - may contain errors
2 protocols using fitc anti human cd324 e cadherin antibody
1
Quantifying Epithelial-Mesenchymal Transition
2
Flow Cytometric Analysis of E-cadherin
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Cells were washed with PBS, detached using Trypsin/EDTA (PAN Biotech, Adenbach, Germany) and washed twice with PBS containing 2% BSA. Cells were stained with 5 µl FITC anti-human CD324 (E-cadherin) antibody (RRID : AB_756066, Biolegend, San Diego, CA, USA) or 5 µl FITC Mouse IgG1 κ Isotype Ctrl antibody (RRID : AB_2861401, Biolegend) for 30 min at 4°C in the dark. After two further washing steps, cells were measured with a Gallios flow cytometer (Beckman Coulter, Brea, CA, USA) and data were analyzed using Kaluza Analysis 2.1 software.
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