The CXCR4 receptor was identified on cells by immunofluorescence staining. Cells were plated in a fibronectin coated 24 well plate at a density of 5000 cells/cm2 and incubated in 500 μl culture medium for 24 hours at 37°C. Cells were fixed in 4% paraformaldehyde for 40 minutes, blocked with 1% BSA overnight at 4°C, incubated with rabbit anti-CXCR4 (Thermo Scientific) (1:200) primary antibody for 45 minutes at 37°C, and incubated with AlexaFluor 488 goat anti-rabbit IgG (Abcam) (1:400) secondary antibody for 45 minutes at 37°C. Nuclei were counterstained with DAPI.
Rabbit anti cxcr4
Manufactured by Thermo Fisher Scientific
Rabbit anti-CXCR4 is a primary antibody that targets the chemokine receptor CXCR4. It can be used to detect and analyze the expression of CXCR4 in various biological samples.
Automatically generated - may contain errors
3 protocols using rabbit anti cxcr4
1
CXCR4 Receptor Immunofluorescence Staining
2
PLA for CXCR4-ADRB2 Heteromers in Glioblastoma PDX
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Example 7
To perform PLA with PDX samples, the glioblastoma patient derived FFPE samples were used (provided by Samsung Seoul hospital in Seoul, Korea). After FFPE sample were de-paraffinized and performed heat induced antigen retrieval for 15 minutes at 100° C. Slides were blocked with blocking solution provided by Duolink and incubated with rabbit anti-CXCR4 (1:200, Thermoscientific, PA3305), mouse anti-ADRB2 (1:200, Santacruz, Sc-271322), at 37° C. for 1 h in a humidifying chamber. The other process was same as described above (PLA with PDC).
In the
3
Personalized PLA for Glioblastoma PDX Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Example 7
To perform PLA with PDX samples, the glioblastoma patient derived FFPE samples were used (provided by Samsung Seoul hospital in Seoul, Korea). After FFPE sample were de-paraffinized and performed heat induced antigen retrieval for 15 minutes at 100° C. Slides were blocked with blocking solution provided by Duolink and incubated with rabbit anti-CXCR4 (1:200, Thermoscientific, PA3305), mouse anti-ADRB2 (1:200, Santacruz, Sc-271322), at 37° C. for 1 h in a humidifying chamber. The other process was same as described above (PLA with PDC).
In the
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