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Apc anti mouse cd206 mmr antibody

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The APC anti-mouse CD206 (MMR) antibody is a flow cytometry reagent that specifically binds to the mouse CD206 antigen, also known as the mannose receptor. CD206 is a transmembrane receptor expressed on the surface of macrophages and dendritic cells, and plays a role in the endocytosis of glycoproteins. This antibody can be used to identify and analyze CD206-positive cells in mouse samples.

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Lab products found in correlation

Fitc anti mouse cd86 antibody, by BioLegend (1 mentions) Software, by FlowJo (1 mentions) Facscelesta, by BD (1 mentions) Apc anti mouse f4 80 antibody, by BioLegend (1 mentions) Fitc anti mouse human cd11b antibody, by BioLegend (1 mentions) Cytoflex flow cytometer, by Beckman Coulter (1 mentions)

2 protocols using apc anti mouse cd206 mmr antibody

1

Intracellular Staining of CD86 and CD206

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For intracellular staining, the cells were blocked by blocking buffer (PBS solution with 5% BSA) and incubated with FITC anti-mouse CD86 antibody (Biolegend, 105005, 1:100) and APC anti-mouse CD206 (MMR) antibody (Biolegend, 141707, 1:100) at 4 °C for 1 h in a dark place. The cells were then washed in PBS and detected by a flow cytometry (BD FACSCelesta, USA). The results were analyzed using FlowJo software (FlowJo LLC, Ashland, OR, USA).
Jia N., Gao Y., Li M., Liang Y., Li Y., Lin Y., Huang S., Lin Q., Sun X., He Q., Yao Y., Zhang B., Zhang Z, & Zhang L. (2023). Metabolic reprogramming of proinflammatory macrophages by target delivered roburic acid effectively ameliorates rheumatoid arthritis symptoms. Signal Transduction and Targeted Therapy, 8, 280.
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2

Multicolor Flow Cytometry Analysis of Immune Cells

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The cells were analysed by cytoFLEX flow cytometer (Beckman Coulter, USA), using the FlowJo software. The antibodies used in present study were FITC anti‐mouse/human CD11B Antibody (Biolegend, Cat. No. 101205, USA), APC anti‐mouse F4/80 Antibody (Biolegend, Cat. No. 123116, USA), APC anti‐mouse CD206 (MMR) Antibody (Biolegend, Cat. No. 141707, USA) and PE anti‐mouse Ly6G Monoclonal Antibody (Elabscience, Cat. No. E‐AB‐F1108D, China).
Li K., Xu W., Lu K., Wen Y., Xin T., Shen Y., Lv X., Hu S., Jin R, & Wu X. (2020). CSF‐1R inhibition disrupts the dialog between leukaemia cells and macrophages and delays leukaemia progression. Journal of Cellular and Molecular Medicine, 24(22), 13115-13128.
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