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3 race system for the rapid amplification of cdna ends

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The 3' RACE System for the Rapid Amplification of cDNA ends is a laboratory equipment designed for the amplification of the 3' end of messenger RNA (mRNA) transcripts. It is used to determine the complete sequence of an mRNA, including the 3' untranslated region, which is important for understanding gene expression and regulation.

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Topo ta cloning, by Thermo Fisher Scientific (2 mentions)

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3′ RACE System (23 citations) 3′ RACE System for Rapid Amplification of cDNA Ends (18 citations) RACE system (12 citations)

2 protocols using 3 race system for the rapid amplification of cdna ends

1

Profiling ISG15 Splice Variants

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted from the patient’s hTERT-immortalized fibroblasts. We performed 3′ RACE PCR with the 3′ RACE System for the Rapid Amplification of cDNA ends (Invitrogen), according to the manufacturer’s instructions. We used the 3′ RACE Universal Amplification Primer (UAP) supplied in the kit, which was complementary to the adaptor primer, and a gene-specific primer that bound to ISG15. PCR products were cloned by TOPO-TA cloning (Thermo Fisher Scientific). Twenty-five colonies were grown. The plasmids isolated were subjected to Sanger sequencing to quantify the different splicing variants.
Martin-Fernandez M., García-Morato M.B., Gruber C., Loza S.M., Malik M.N., Alsohime F., Alakeel A., Valdez R., Buta S., Buda G., Marti M.A., Larralde M., Boisson B., Rodriguez M.F., Qiu X., Chrabieh M., Ayed M.A., Muhsen S.A., Desai J.V., Ferre E.M., Rosenzweig S.D., Amador-Borrero B., Bravo-Gallego L.Y., Olmer R., Merkert S., Bret M., Sood A.K., Al-rabiaah A., Temsah M.H., Halwani R., Hernandez M., Pessler F., Casanova J.L., Bustamante J., Lionakis M.S, & Bogunovic D. (2020). Systemic Type I IFN Inflammation in Human ISG15 Deficiency Leads to Necrotizing Skin Lesions. Cell reports, 31(6), 107633.
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2

Profiling ISG15 Splice Variants

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted from the patient’s hTERT-immortalized fibroblasts. We performed 3′ RACE PCR with the 3′ RACE System for the Rapid Amplification of cDNA ends (Invitrogen), according to the manufacturer’s instructions. We used the 3′ RACE Universal Amplification Primer (UAP) supplied in the kit, which was complementary to the adaptor primer, and a gene-specific primer that bound to ISG15. PCR products were cloned by TOPO-TA cloning (Thermo Fisher Scientific). Twenty-five colonies were grown. The plasmids isolated were subjected to Sanger sequencing to quantify the different splicing variants.
Martin-Fernandez M., García-Morato M.B., Gruber C., Loza S.M., Malik M.N., Alsohime F., Alakeel A., Valdez R., Buta S., Buda G., Marti M.A., Larralde M., Boisson B., Rodriguez M.F., Qiu X., Chrabieh M., Ayed M.A., Muhsen S.A., Desai J.V., Ferre E.M., Rosenzweig S.D., Amador-Borrero B., Bravo-Gallego L.Y., Olmer R., Merkert S., Bret M., Sood A.K., Al-rabiaah A., Temsah M.H., Halwani R., Hernandez M., Pessler F., Casanova J.L., Bustamante J., Lionakis M.S, & Bogunovic D. (2020). Systemic Type I IFN Inflammation in Human ISG15 Deficiency Leads to Necrotizing Skin Lesions. Cell reports, 31(6), 107633.
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