Nmri foxn1nu foxn1nu mice

B16/F1 cells (3x10⁶) in a volume of 150 μL 0.9% NaCl were inoculated subcutaneously on the right shoulder of NMRI-Foxn1^nu/Foxn1^nu mice (Janvier Labs, Saint-Berthevin, France). After 1–2 weeks of growth, tumor bearing mice were injected with approximately 15 MBq of ⁶⁸Ga-DOTA-NAPamide in a volume of approximately 150 μL 0.9% NaCl into a lateral tail vein via a catheter. Positron emission tomography (PET) / magnetic resonance imaging (MRI) (1 Tesla nanoScan PET/MRI Mediso, Budapest, Hungary) was performed at Berlin Experimental Radionuclide Imaging Center (BERIC), Charité –Universitätsmedizin Berlin. Anatomic MRI scans were acquired using a T2-weighted 2D fast spin echo sequence (T2 FSE 2D) with the following parameters: coronal sequentially, matrix 256x256x20 with dimensions 0.36x0.36x1.5mm³, TR: 8695 ms, TE: 103 ms, and a flip angle of 180°. PET scans were performed for 90 min starting directly before intravenous injection of tracer. PET images were reconstructed from the raw data with the following image sequence: 6 x 10 s, 6 x 30 s, 5 x 60 s and 8 X 600 s. The tracer standardized uptake value (SUV) in the tumor tissue was determined by manual contouring of a volume of interest (VOI) of the PET images using PMOD 3.610 (PMOD Technologies, Zürich, Switzerland).

NMRI-Foxn1^nu/Foxn1^nu mice were provided by Janvier Laboratories (Le Genest-Saint-Isle, France). Mice were kept within the Experimental Animal House of the Centre de Cancérologie de Marseille (CRCM) pôle Luminy. All experimental protocols were carried out in accordance with nationally approved guidelines for the treatment of laboratory animals. All experimental procedures on animals were approved by the Comité d’éthique de Marseille numéro 14. Ten millions MiaPaCa-2 cells were inoculated subcutaneously and mice were separated into 3 groups of 6 subjects each. Mice were treated daily with either physiologic serum, 5 mg/kg or 10 mg/kg of the compound when the tumor volume reached approximately 400 mm³. Every 5 days, the weight and the tumors volumes were measured. Mice were sacrificed after 35 days of treatment.

For in vivo experiments, at least 8-week-old female athymic NMRI-Foxn1^nu/Foxn1^nu mice (Janvier Labs, Saint-Berthevin, France) were used. Animal care followed institutional guidelines and all experiments were approved by local animal research authorities. For the generation of tumor xenografts, 5 x 10⁶ cells of Capan-2 cells were inoculated subcutaneously into the left and right shoulder (1:1 phosphate-buffered saline [PBS]/Matrigel Basement Membrane Matrix High Concentration, Corning, Corning, USA). Tumors were allowed to grow for two to four weeks (tumor volume > 100 mm³) after cell inoculation.