Ferrous chloride fecl2 4h2o

Manufactured by Merck Group

Sourced in United Kingdom

Ferrous chloride (FeCl2·4H2O) is an inorganic compound that consists of iron (II) ions and chloride ions. It is a crystalline solid that is soluble in water and other polar solvents. Ferrous chloride is commonly used as a coagulant in water treatment and as a reducing agent in various chemical processes.

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5 protocols using ferrous chloride fecl2 4h2o

Biopolymer Hydrogel for Antimicrobial Therapy

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Sodium alginate, casein (from bovine milk), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxy-succinimide (NHS), agar powder, calcium chloride (CaCl₂), ferrous chloride (FeCl₂·4H₂O) (>98%), ferric chloride (FeCl₃·6H₂O) (>98%), sodium hydroxide (NaOH) (pellets, >98%) and dimethylformamide (DMF) (>99%) were supplied by Sigma-Aldrich, Praguel, Czech Republic. Ammonium hydroxide (NH₄OH) (30% solution) was purchased from Fluka, Neu-Ulm, Germany. BC used in this study was produced by strain Gluconacetobacter xylinus 3611T procured from Czech Collection of Microorganisms (CCM), Brno, Czech Republic. Minocyclin hydrochloride 50 mg capsules were purchased from Stada Pharma, Prague, Czech Republic. All chemicals were used without any further purification. Double distilled water was used throughout the experimental studies.

Patwa R., Zandraa O., Capáková Z., Saha N, & Sáha P. (2020). Effect of Iron-Oxide Nanoparticles Impregnated Bacterial Cellulose on Overall Properties of Alginate/Casein Hydrogels: Potential Injectable Biomaterial for Wound Healing Applications. Polymers, 12(11), 2690.

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Synthesis of Iron Oxide Nanoparticles

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All the reagents used in this work including ferrous chloride FeCl₂.4H₂O (M.W. 198.81), ferric chloride FeCl₃.6H₂O, and sodium hydroxide (NaOH) were of analytic grade and purchased from Sigma Aldrich, UK.

Asimeng B.O., Nyankson E., Efavi J.K., Nii Amarkai A., Manu G.P, & Tiburu E. (2020). Characterization and Inhibitory Effects of Magnetic Iron Oxide Nanoparticles Synthesized from Plant Extracts on HeLa Cells. International Journal of Biomaterials, 2020, 2630735.

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Synthesis of Polyaniline Nanocomposites

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All chemicals were of analytical grade. Polyvinyl alcohol with a degree of hydrolysis of 89% (molecular weight, M_w, of about 115.000 g mol⁻¹, Sigma-Aldrich), ferrous chloride (FeCl₂·4H₂O (Sigma-Aldrich)), ferric chloride (FeCl₃·6H₂O) (Sigma-Aldrich), sodium hydroxide (NaOH) (Sigma-Aldrich), deionized water, aniline (Sigma-Aldrich), dodecylbenzene sulfonic acid (DBSA), and ammonium persulfate ((NH₄)₂S₂O₈) (Sigma-Aldrich) were used without further purification.

Almeida Moraes T., Farrôco M.J., Pontes K., Fontes Bittencourt M., Guenter Soares B, & Gomes Souza F J.r. (2020). An optical-magnetic Material as a toxic gas filter and sensing device. RSC Advances, 10(39), 23233-23244.

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Analytical-grade Reagent Synthesis

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Analytical-grade K₂Cr₂O₇ (≥ 99.0%), Orange II sodium salt (AO7 dye) (dye content ≥ 85 %), ferrous chloride (FeCl₂.4H₂O) (≥ 99.0%), sodium hydroxide (NaOH) (≥ 98%), and ferric chloride (FeCl₃·6H₂O) (97%) were purchased from Sigma-Aldrich. All the solutions were prepared in distilled water.

Santhosh C., Daneshvar E., Tripathi K.M., Baltrėnas P., Kim T., Baltrėnaitė E, & Bhatnagar A. (2020). Synthesis and characterization of magnetic biochar adsorbents for the removal of Cr(VI) and Acid orange 7 dye from aqueous solution. Environmental Science and Pollution Research International, 27(26), 32874-32887.

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Ferrous Chloride Infection in Mice

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Swiss-Webster mice (n = 5/group) were injected with 75 μg of ferrous chloride (FeCl₂·4H₂O; Sigma-Aldrich Inc., St. Louis, MO) by the intraperitoneal (i.p.) route (65 (link)) and then challenged i.n. with either the KIM/D27 strain or the LMA vaccine strain (2 × 10⁵ to 5 × 10⁶ CFU/50 μl). A similar number of untreated mice (without FeCl₂·4H₂O) were also infected and served as controls. The animals were observed for body weight loss, other clinical signs of disease (ruffled fur, hunched back, lethargy, lack of grooming, sunken eyes, squinting of eyes with ocular and/or nasal discharge, open-mouth breathing, and gasping for air), and morbidity over a period of 14 days.

Kilgore P.B., Sha J., Hendrix E.K., Motin V.L, & Chopra A.K. (2021). Combinatorial Viral Vector-Based and Live Attenuated Vaccines without an Adjuvant to Generate Broader Immune Responses to Effectively Combat Pneumonic Plague. mBio, 12(6), e03223-21.

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