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Wako labassay glucose kit

Manufactured by Fujifilm
Sourced in United States, Japan

The Wako Labassay™ Glucose kit is a laboratory reagent used for the quantitative measurement of glucose levels in various biological samples. It provides a standardized and reliable method for glucose determination.

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3 protocols using wako labassay glucose kit

1

Glucose Metabolism and Signaling

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K597, a DPP-4 inhibitor, and the Wako Labassay™ Glucose kit were from Wako Pure Chemical. Primary antibodies against GLUT1 and GLUT4, horseradish peroxidase-conjugated anti-goat, anti-rabbit and anti-mouse IgG antibodies and protein A/G plus-agarose were obtained from Santa Cruz Biotechnology Inc. Anti-AMPKα, anti-PI3K, anti-Akt and anti-insulin receptor (IR)β were from Cell Signaling Technology Inc. Anti-phosphotyrosine and anti-insulin receptor substrate (IRS)-1 were from Becton, Dickinson and Company. All other reagents used were of the highest grade available from commercial sources.
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2

Metabolic Profile Assessment in Rats

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Blood was collected from the retro-orbital venous plexus of overnight-fasted rats. Plasma glucose, free fatty acid, triglyeride and total cholesterol levels were measured using a Wako LabAssay glucose kit (298-65701), LabAssay NEFA kit (294-63601), LabAssay Triglyceride (290-63701) and LabAssay Cholesterol kit (294-65801) (Wako Chemicals USA, Inc.), respectively.
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3

Blood Glucose and Insulin Assay

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Blood glucose levels were determined using the Wako LabAssay™ Glucose kit (Wako Pure Chemical Industries, Ltd., Japan), according to the manufacturer’s protocol. Serum insulin levels were measured using the BlueGene Rat Insulin enzyme-linked immunosorbent assay kit (Shanghai BlueGene Biotech CO., Ltd., China), according to the manufacturer’s protocol.
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