Flow cytometry was performed on 1E6 stained cells from each enriched fraction, including a non-enriched sample from each tonsil for comparison. Surface and intracellular flow cytometry was performed as previously described12 (link) on a Becton Dickenson LSR II flow cytometer using FACS Diva analysis software (BD Biosciences). The following commercially available antibodies were used: CD8-PE, CD19-PE, CD19-FITC, CD94-APC, CD3-APC-H7, and IFN-γ-BV421 (BD Biosciences); CD3-PerCP-Vio700, CD14-PerCP-Vio700, CD117-PE-Vio770, CD3-VioGreen, and CD14-VioGreen (Miltenyi Biotec); and IL-22-PE (R&D Systems). Cytofix/Cytoperm and PermWash reagents (BD Biosciences) were used to perform intracellular flow cytometry as previously described12 (link).
Cd14 percp vio700
Manufactured by Miltenyi Biotec
Sourced in United States
The CD14-PerCP-Vio700 is a fluorochrome-conjugated antibody from Miltenyi Biotec used for the identification and enumeration of CD14-positive cells in flow cytometric analysis. It binds to the CD14 antigen, a glycosylphosphatidylinositol-anchored protein expressed on the surface of myeloid cells, such as monocytes and macrophages.
Automatically generated - may contain errors
Lab products found in correlation
Cd3 percp vio700, by Miltenyi Biotec (2 mentions)
Cd3 viogreen, by Miltenyi Biotec (1 mentions)
Facsdiva analysis software, by BD (1 mentions)
Cd3 apc h7, by BD (1 mentions)
Cd94 apc, by BD (1 mentions)
Ifn γ bv421, by BD (1 mentions)
Cd117 pe vio770, by Miltenyi Biotec (1 mentions)
Cd14 viogreen, by Miltenyi Biotec (1 mentions)
Cd19 pe, by BD (1 mentions)
Cd19 fitc, by BD (1 mentions)
Cytofix cytoperm, by BD (1 mentions)
Cd8 pe, by BD (1 mentions)
Pd 1 apc cy7, by BioLegend (1 mentions)
Nkg2d pe, by BioLegend (1 mentions)
Macsquant10 analyzer, by Miltenyi Biotec (1 mentions)
Cd62l pe cy7, by BioLegend (1 mentions)
Cd56 pe dazzle 594, by BioLegend (1 mentions)
Klrg1 apc fire 750, by BioLegend (1 mentions)
Ifn γ pe cy7, by BioLegend (1 mentions)
Nkp44 apc, by BioLegend (1 mentions)
2 protocols using cd14 percp vio700
1
Flow Cytometry of Immune Cells
2
Multiparameter Flow Cytometry Immunophenotyping
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The following fluorescence-labeled monoclonal antibodies (mAb) were used: CD3-FITC or PE/Cy5 (clone UCHT1), CD11c-FITC (3.9), CD16-APC/Cy7 (3G8), CD30-PE/Cy7 (BY88), CD33-PE, BV605 or PE/Cy5 (P67.6), CD56-PE/Dazzle™594 (N901), CD57-FITC (HCD57), CD62L-PE/Cy7 (DREG56), CD107-BV510 (H4A3), CD117-BV421 (104D2), KLRG1-APC/Fire 750 (SA231A2), PD1-APC/Cy7 (EH12-2H7), NKG2D-PE (1D11), NKp46-BV510 (9E2), NKp44-APC (P44-8), NKp30-BV785 (P30-15), Granzyme B-Pacific Blue (GB11), IFNγ PE/Cy7 (B27), Perforin-PE (dG9), TNFα PE/Dazzle™ 594 (all from Biolegend, CA, USA), CD3 PerCP-Vio700 (REA613), CD14-PerCP-Vio700 (REA599), CD33-APC, CD56 (REA196), anti-biotin-VioBright515 and 7-AAD (all Miltenyi Biotec), NKG2C-AF700 (134591) from R&D systems (MN, USA), and CD158b1,b2,j-PE/Cy5 (GL183), NKG2A-APC (Z199) purchased from Beckman Coulter (CA, USA). Flow cytometric analyses were performed on a CytoFLEX (Beckman Coulter) or MACSQuant 10 Analyzer (Miltenyi Biotec). Data analysis was performed on Kaluza 2.1.1 software.
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