Sc19220

L-phenylephrine hydrochloride, acetylcholine chloride, sodium nitroprusside, L-NAME, apocynin, indomethacin, SOD, losartan, salts and other reagents were purchased from Sigma Chemical Co., and Merck (Darmstadt, Germany). NS398, SQ29548, SC19220, and CAY10441 were purchased from Cayman Chemical (Ann Arbor, MI, United States). Lead acetate was obtained from Vetec (Rio de Janeiro, RJ, Brazil). All drugs were dissolved in distilled water except NS398, SC19220, and CAY10441, which were dissolved in DMSO, and SQ29548, which was dissolved in ethanol. DMSO and ethanol did not have any effects on the parameters evaluated for vascular reactivity.

L-NAME, ACh, PE, AA, and the non-selective COX inhibitor indomethacin were purchased from Sigma (St Louis, MO, USA). The TP agonist U46619, PGI₂, PGF_2α, PGE₂, and PGD₂, the TP antagonist SQ29548, the IP antagonist CAY10441, the EP3 antagonist L798106, and the EP1 antagonist, SC19220 were bought from Cayman Chemical (Ann Arbor, MI, USA). The composition of physiological salt solution (PSS; pH 7.4 with 95%O₂–5% CO₂) was as follows (in mM): NaCl 123, KCl 4.7, NaHCO₃ 15.5, KH₂PO₄ 1.2, MgCl₂ 1.2, CaCl₂ 1.25, and D-glucose 11.5. The 60 mM K⁺ -PSS (K⁺) was prepared by replacing an equal molar of NaCl with KCl.
L-NAME, PE, AA, and ACh were dissolved in distilled water (purged with N₂ for dissolving AA), while PGI₂ was dissolved in carbonate buffer (50 mM, pH 10.0). PGF_2α, PGE₂, PGD₂, CAY10441, SQ29548, L798106, and indomethacin were dissolved in dimethyl sulfoxide (DMSO). The final ratio of a solvent (distilled water, carbonate buffer, or DMSO) to working PSS was 0.5/1,000, which doesn’t alter the final pH value of the working buffer (pH 7.4). The concentration of an inhibitor or antagonist used was based on previous reports, which would selectively inhibit the effect of its intended target27 (link)54 (link)55 (link).

AlCl₃·6H₂O (Sinopharm Chemical Reagent Co., Ltd., China) and maltol (Aladdin, USA) were of analytical grade. Maltol solution(60 mM) was prepared by adding 0.3784g maltol into 50 ml of autoclaved PBS and 0.1207g AlCl₃·6H₂O was added into 25 ml of autoclaved PBS as AlCl₃ solution(20 mM). Aluminum-maltolate solution (10 mM) was prepared by adding 25ml maltol solution and 25 ml AlCl₃ solution. After filtered through 0.22 mm millipore filter, 60 mM maltol solution and 10 mM aluminum-maltolate solution was stored at 4°C until used [24 (link), 78 (link)]. SC19220, AH6809 and L-161982 (Cayman, USA) were dissolved in the DMSO (Sigma, USA) to be 10 mM reserve liquid. 17-phenyl trinor Prostaglandin E2 ethyl amide and CAY10598 (Cayman, USA) were dissolved in ethyl alcohol to be 10 mM reserve liquid. Butaprost and Sulprostone (Cayman, USA) were dissolved in methyl acetate to be 10 mM reserve liquid [24 (link)].