8890 gas chromatograph

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 8890 gas chromatograph is a laboratory instrument designed for the separation and analysis of chemical compounds. It utilizes a gas carrier and heated column to separate a sample into its individual components, which are then detected and measured.

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Gas chromatograph (43 citations)

5 protocols using 8890 gas chromatograph

HS-GC-MS Analysis of Volatile Compounds

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HS-GC-MS analyses were carried out on an 8890 gas chromatograph (Agilent Technologies, Santa Clara, CA, USA) equipped with an HS system (Gerstel, Mülheim, Germany) and coupled to a mass spectrometer (5977B) quadrupole mass selective detector (Agilent Technologies) with inert ion source based on electron impact (EI). The chromatographic separation was carried out on an HP-5MS capillary column (30 m × 0.25 mm inner diameter × 0.25 µm film thickness), also from Agilent Technologies.
Analysis and data acquisition were carried out using the MSD Chemstation Data Analysis application (Version G1701EA), and data were processed using MS-DIAL (Version 4.80, RIKEN) and SIMCA-P (Umetrics, Malmö, Sweden).

Arroyo-Manzanares N., García-Nicolás M., Abellán-Alfocea F., Prieto-Baeza L., Campillo N., del Val Oliver B., Zarauz-García J., Sáenz L, & Viñas P. (2023). Application of untargeted volatile profiling in inflammatory bowel disease research. Analytical and Bioanalytical Chemistry, 415(17), 3571-3579.

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GC-MS Analysis of Volatile Compounds

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The volatile compounds were separated on a DB-5MS column (30 m × 0.25 mm × 0.25 μm film thickness; Agilent J&W Scientific, Folsom, CA, USA) at splitless mode installed on an Agilent 8890 gas chromatograph equipped with a Agilent 7000D mass spectrometer (Santa Clara, CA, USA). The GC–MS system was controlled and operated using an Agilent Mass-hunter. Helium (99.999 %) was selected as the carrier gas at a linear velocity of 1.2 mL/min. The temperatures of 250 °C were set for the injector. To ensure the complete desorption of volatile components, the SPME fiber need to be inserted into the heated GC injector after sampling and maintained at 250 °C for 5 min. The temperature program of column was set as follows: first, the initial column temperature was maintained at 40 °C for 3.5 min, followed by a ramp of 10 °C/min to 100 °C, then a ramp of 7 °C/min to 180 °C, finally increased to 280 °C at a rate of 25 °C/min, and held at 280 °C for 5 min. For MS, selected ion monitoring (SIM) mode was used for the identification and quantification of analytes. The ionization voltage was 70 eV, and the temperatures of ion source, quadrupole mass detector and transfer line were 230, 150 and 280 °C, respectively. The total ionic chromatogram (TIC) with m/z range of 50–450 was obtained (Supplementary Fig. 1).

Liu H., Cheng Z, & Xie J. (2024). Formation of special odors driven by volatile compounds during the growth and maturation in edible fungi (Phallus impudicus). Food Chemistry: X, 22, 101288.

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GC-MS Analysis of Volatile Compounds in Honey

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An 8890-gas chromatograph from Agilent Technologies (CA, USA) with a multi-purpose sampler (MPS) operating in headspace mode and a 2.5 mL syringe (Gerstel, Mülheim, Germany) were coupled to a 5977B-quadrupole mass spectrometer with an inert ion source also from Agilent. Chromatographic separation was performed using two in-line Agilent HP-5MS capillary columns (5% diphenyl-95% dimethylpolysiloxane) with 15 m × 0.25 mm I.D. × 0.25 μm, combined with a backflush system setting 0.83 min as post run time.
MassHunter Workstation software (Qualitative Analysis version B.08.00) from Agilent Technologies was used for data acquisition. StatGraphics Plus 5.1 (Statistical Graphics, Rockville, MD, USA), MS-DIAL 4.80 and SIMCA 14.1 (Umetrics, Umeå, Sweden) software were used for the processing of data. The NIST mass spectral library was used for the identification of VOCs.
For the homogenization of honey samples before analysis, an LLG-uniTEXER vortex agitator (Heathrow Scientific, Vernon Hills, Chicago, IL, USA) was used.

Castell A., Arroyo-Manzanares N., Guerrero-Núñez Y., Campillo N, & Viñas P. (2023). Headspace with Gas Chromatography-Mass Spectrometry for the Use of Volatile Organic Compound Profile in Botanical Origin Authentication of Honey. Molecules, 28(11), 4297.

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Volatile Organic Compound Analysis by GC-MS

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Approximately 50 mL of each gas
sample was collected to a Tenax tube (TA 200 mg 35/60 mesh inert coated
conditioned stainless-steel TD tube) and analyzed using a TD100-XR
GC system coupled to an 8890 gas chromatograph (Agilent) with 5977B
MSD (Agilent). The column was Agilent HP-5MS (30 m, 0.25 mm, 0.25
μm). Pretrap fire purging was performed for 1 min, after which
the trap was fired at 300 °C for 3 min. Split flow during trap
desorption was 50 mL min^–1, resulting in a split
ratio of 42:1. Helium was applied as a carrier gas at 1.2 mL min^–1. The column program was started at 40 °C, which
was held for 7 min, and increased at a rate of 10 °C min^–1 to 150 °C and then 40 °C min^–1 to 325 °C, which was held for 7 min, giving a total run time
of 27.3 min.

Hongthong S., Leese H.S, & Chuck C.J. (2020). Valorizing Plastic-Contaminated Waste Streams through the Catalytic Hydrothermal Processing of Polypropylene with Lignocellulose. ACS Omega, 5(32), 20586-20598.

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Rapid GC-MS System Configuration

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All analyses were completed using an Agilent 3971 QuickProbe GC-MS system coupled to an Agilent 8890 gas chromatograph and a 5977B mass spectrometer, equipped with a 7693 autosampler (Agilent Technologies, Santa Clara, CA, USA). Configuration of the rapid GC-MS column such that the MS could be used by both the rapid and traditional liquid injection GC was done using an inert splitter (Supplementary Data, Figure S1). The bottom port of the splitter was connected to the MS through a DB-1ms Ultra Inert QuickProbe GC column (1 m length × 0.18 mm outer diameter × 0.18 μm inner diameter). The middle port was connected to the rapid GC column, a DB-1ht QuickProbe GC column (2 m length × 0.25 mm outer diameter × 0.10 μm inner diameter), which is housed inside the rapid system attachment. The top port of the splitter was connected to the traditional, liquid-injection GC column, a 100 % polydimethylsiloxane column (DB-1, 30 m length × 0.25 mm outer diameter × 0.25 μm inner diameter, Agilent Technologies). For both rapid and traditional GC, helium (99.999 %) was used as the carrier gas with a flow rate of 1 mL/min.

Capistran B.A, & Sisco E. (2022). Rapid GC-MS as a Screening Tool for Forensic Fire Debris Analysis. Forensic chemistry (Amsterdam, Netherlands), 30, 10.1016/j.forc.2022.100435.

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