For fluorescence microscopy, 500 μL of cultured cells were removed from the flask at the desired time points, centrifuged for 3 min at 5000 × g, and resuspended in an appropriate volume of water. A total of 4 μL of each sample was spotted on a microscope slide and viewed using an Olympus 1 × 51 inverted fluorescence microscope.
51 inverted fluorescence microscope
Manufactured by Olympus
Sourced in Japan
The 1x51 inverted fluorescence microscope is a laboratory equipment designed for microscopic analysis. It features an inverted optical configuration, allowing for the observation of samples from below. The microscope is capable of fluorescence imaging, enabling the visualization of fluorescently labeled specimens.
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3 protocols using 51 inverted fluorescence microscope
1
Fluorescence Microscopy of Cultured Cells
2
Methylene Blue Viability Assay
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Cultured cells (1 mL) were collected and stained with 0.025% methylene blue (Sigma-Aldrich) for 5 minutes. Samples (10 µL) were then placed on a hemocytometer and viewed using an Olympus (Tokyo, Japan) 1×51 inverted fluorescence microscope equipped with U-MWU filters (330–385/400/420 nm). Dead cells were stained, while alive cells remained completely unstained.
3
Fluorescence and TEM Microscopy Protocol
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For fluorescence microscopy, 500 µL of cultured cells were removed from the flask at the desired time points, centrifuged for 3 minutes at 5,000× g, and resuspended in an appropriate volume of water. A total of 4 µL of each sample was spotted on a microscope slide and viewed using an Olympus 1×51 inverted fluorescence microscope.
For transmission electron microscopy (TEM), samples were observed under an FEI Tecnai G2 20 TWIN transmission electron microscope (FEI, Eindhoven, the Netherlands).
For transmission electron microscopy (TEM), samples were observed under an FEI Tecnai G2 20 TWIN transmission electron microscope (FEI, Eindhoven, the Netherlands).
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