Ab124798

Manufactured by Abcam

Sourced in United Kingdom

Ab124798 is a lab equipment product. It is a device used for performing specific laboratory tasks. The core function of this product is to facilitate certain experimental or analytical procedures in a laboratory setting.

Automatically generated - may contain errors

Lab products found in correlation

A28175, by Thermo Fisher Scientific (1 mentions) Ab184919, by Abcam (1 mentions) Peroxidase conjugated secondary antibody, by Merck Group (1 mentions) Chemiluminescence substrate, by Roche (1 mentions) Memcode, by Thermo Fisher Scientific (1 mentions) Whatman, by Cytiva (1 mentions)

2 protocols using ab124798

Immunostaining and Protein Analysis Techniques

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Immunostaining (confocal microscopy), immunoblotting, and in-cell Western blottings were performed using primary antibodies recognizing β-arrestin 1/2 (Cell Signaling Technology 46745; rabbit, 1:1000), β-actin (Cell Signaling Technology 3700S; mouse, 1:5000), α-tubulin (Cell Signaling Technology 3873;mouse, 1:4000), β-tubulin (Cell Signaling Technology 2146S; rabbit, 1:500), GRK2 (Santa Cruz Biotechnology sc-562; rabbit, 1:200), Rac1 (Millipore 05-389; mouse, 1:4000), DSC1 (Abcam ab150382; rabbit, 1:1000), DSG1 (Abcam ab124798; rabbit, 1:1000), IQGAP1 (Abcam ab86064; rabbit, 1:2000), JUP (Abcam ab184919; rabbit, 1:1000), CRKL (Abcam ab151791; rabbit, 1:1000), FLAG M2 for confocal microscopy (Sigma F3165; mouse, 1:1000), and FLAG M2 for in-cell Western blottings (Sigma F3165; mouse, 1:200).
Primary antibodies were detected using the following fluorescent secondary antibodies: goat anti-mouse 680 (LI-COR 926-68070; 1:10,000 for immunoblotting or 1:800 for in-cell Western blottings); goat anti-rabbit 800 (LI-COR 926-32211; 1:10,000 for immunoblotting or 1:800 for in-cell Western blottings); goat anti-mouse AlexaFluor 488 (ThermoFisher Scientific A28175; 1:500 for confocal microscopy). Endogenous Rac1 and α-tubulin (immunoblotting) were detected using a HRP-linked antibody (Cell Signaling Technology 7076; mouse, 1:4000).

Civciristov S., Huang C., Liu B., Marquez E.A., Gondin A.B., Schittenhelm R.B., Ellisdon A.M., Canals M, & Halls M.L. (2019). Ligand-dependent spatiotemporal signaling profiles of the μ-opioid receptor are controlled by distinct protein-interaction networks. The Journal of Biological Chemistry, 294(44), 16198-16213.

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Proteomic Profiling of Dystrophin Complex

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For the gel-and liquid chromatography-based proteomic and biochemical profiling of the dystrophin complex and membrane fractions from skeletal muscles, analytical grade reagents and materials were purchased from GE Healthcare (Little Chalfont, Buckinghamshire, UK), Sigma Chemical Company (Dorset, UK), Bio-Rad Laboratories (Hemel-Hempstead, Hertfordshire, UK) and National Diagnostics (Atlanta, GA, USA). Proteolytic digestion was carried out with sequencing grade modified trypsin and Lys-C enzymes from Promega (Madison, WI, USA). Materials for immunoblotting included Whatman nitrocellulose transfer membranes from Invitrogen (Carlsbad, CA, USA), chemiluminescence substrate from Roche Diagnostics (Mannheim, Germany) and the reversible membrane stain MemCode from Thermo Fisher Scientific (Waltham, MA, USA). Primary antibodies were purchased from Abcam, Cambridge, UK (ab124798 to desmoglein isoform DSG1, ab2413 to fibronectin, ab58562 to biglycan, ab52488 to lactate dehydrogenase, ab14739 to the voltage-dependent anion-selective channel protein VDAC-1, and ab16048 to lamin-B1), and Sigma-Aldrich, Dorset, UK (L9393 to laminin). Peroxidase-conjugated secondary antibodies were obtained from Chemicon International (Temecula, CA, USA).

Murphy S., Brinkmeier H., Krautwald M., Henry M., Meleady P, & Ohlendieck K. (2017). Proteomic profiling of the dystrophin complex and membrane fraction from dystrophic mdx muscle reveals decreases in the cytolinker desmoglein and increases in the extracellular matrix stabilizers biglycan and fibronectin. Journal of muscle research and cell motility, 38(2).

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