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2 7 dichlorodihydrofluorescein diacetate dcfh da tert butyl hydroperoxide t buooh 6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox

Manufactured by Merck Group

2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) is a cell-permeant fluorogenic probe used to detect reactive oxygen species (ROS) in cells. tert-Butyl hydroperoxide (t-BuOOH) is an organic peroxide compound used as an oxidizing agent. 6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) is a water-soluble vitamin E analog that functions as an antioxidant.

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2 protocols using 2 7 dichlorodihydrofluorescein diacetate dcfh da tert butyl hydroperoxide t buooh 6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox

1

Assessing Cellular Oxidative Stress

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Dulbecco’s Modified Eagle Medium (DMEM) and Fetal Bovine Serum (FBS) were obtained from Gibco (Paisley, UK). L-glutamine was purchased from Life Technologies. Penicillin, streptomycin, 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA), tert-butyl hydroperoxide (t-BuOOH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) and DMSO were purchased from Sigma Aldrich.
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2

Assessing Cellular Oxidative Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dulbecco’s Modified Eagle Medium (DMEM) and Fetal Bovine Serum (FBS) were obtained from Gibco (Paisley, UK). L-glutamine was purchased from Life Technologies. Penicillin, streptomycin, 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA), tert-butyl hydroperoxide (t-BuOOH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) and DMSO were purchased from Sigma Aldrich.
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