After treatment, the intracellular ATP content was assessed using a luciferase ATP detection assay kit (Beyotime, China) according to the manufacturer's protocol. Briefly, the cells were harvested and lysed. Then, the protein content of the supernatant was determined with a BCA protein kit (Beyotime, China), and the protein supernatant was mixed with an ATP detection solution. Finally, the luminescence of each sample was measured by a fluorescence microplate reader.
Luciferase atp detection assay kit
Manufactured by Beyotime
Sourced in China
The Luciferase ATP detection assay kit is a laboratory tool designed to quantify the amount of ATP (adenosine triphosphate) present in a sample. The kit utilizes the enzyme luciferase, which catalyzes a reaction that produces light in the presence of ATP. This light emission is then measured and used to determine the ATP concentration.
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Lab products found in correlation
2 protocols using luciferase atp detection assay kit
1
Intracellular ATP Quantification Protocol
2
Cellular ATP Content Quantification
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After treatment, the intracellular ATP content was assessed using a luciferase ATP detection assay kit (Beyotime, China) according to the manufacturer’s protocol. Briefly, the cells were harvested and lysed. Then, the protein content of the supernatant was determined with a BCA protein kit (Beyotime, China), and the protein supernatant was mixed with ATP detection solution. Finally, the luminescence of each sample was measured by a fluorescence microplate reader.
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