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13 protocols using cdaq 9171

1

Measuring Force and Pressure in 3D Printed Artificial Arteries

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A force of 30 N was applied to the 3D printed films covered with electrodes by a permanent magnet shaker (LV201, M4-CE) at 1 Hz controlled by a function generator (DS 345, Stanford Research Systems). The force was quantified by a portable sensor measurement system (compression piezoelectric sensor (CL-YD-303) integrated with a four-channel dynamic signal acquisition module (NI 9234) and compact data acquisition chassis (NI, cDAQ-9171)). The real-time pressure of artificial artery system was quantified by a force resistive sensor (FSR 402, Interlink Electronics).
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2

Quantitative Tissue Indentation Protocol

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A custom cantilever-based indenter (Fig. 2i) [15 (link),20 ] was used to indent tissue samples and record force relaxation over time. A piezoelectric stage (P-628.1CD, Physik Instrumente) displaced a soft titanium cantilever with a 3 mm-diameter rigid tip. Cantilever stiffness (175.4 N/m) was calibrated directly with small weights hung from the cantilever tip. A custom program in LabVIEW (National Instruments) was used to control indentation profile and to read deflection of cantilever tip with capacitive sensor (C8S-3.2-2.0 and compact driver CD1–CD6, Lion Precision) through a data acquisition card system (NI 9220 and cDAQ-9171, National Instruments). The cantilever base was driven to a depth of 60 µm at 6 µm/s and then held for 120 s (Fig. 3). Because isolated tissues from animals are hard and expensive to obtain, indentation profiles included a modest loading rate that would allow 10 s of data acquisition at 10 Hz with which to fit indentation models in addition to a relaxation phase that reached a quasi-steady state. This afforded flexibility in choosing models to describe tissue behavior with an efficient use of resources. Samples were indented at 4 different locations along the length of the tissue.
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3

Ginkgo biloba Leaf Extract Dripping Pill Production

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Three batches of Ginkgo biloba leaf extracts which were supplied by two raw material suppliers (two batches were supplied by Jiangsu Beisikang Pharmaceutical Co., Ltd., Xuzhou, China; and one batch was supplied by Zhejiang Conba Pharmaceutical Co., Ltd., Hangzhou, China) were used as active pharmaceutical ingredients (API). The polyethylene glycol (PEG) 4000 (supplied by Wanbangde Pharmaceutical Group, Wenling, China) was used as an excipient. The dimethyl silicone oil was used as condensing oil (purchased from Jiangxi Alpha Hi‐tech Pharmaceutical Co., Ltd., Pingxiang, China).
The width of the droplets was measured using a laser micrometer (KEYENCE IG‐028, Shanghai, China) which is equipped with a sensor amplifier (KEYENCE IG‐1000, Shanghai, China). The data acquisition was performed using a data acquisition card (National Instrument cDAQ‐9171, Shanghai, China). The homogenous dispersing liquid was prepared using a circulating oil bath (Greatwall Scientific SY‐20, Zhengzhou, China) and an electric mixer (Zhengrong instrument ES‐60 M, Changzhou, China). The preparation of dripping pills was performed using a dripping device (Anruikang, Beijing, China). The dripping pills were weighed using an electronic balance (Mettler Toledo AE240, Shanghai, China).
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4

Characterizing Ferroelectric Structure Mechanics

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An instant force of 100 N (peak value) was applied to the 3D-printed ferroelectric structures by an actuator (LinMot USA, Inc.) at 1 Hz controlled by a computer. The tip of the actuator contacting the ferroelectric print has a surface area of ~1.8 cm2. The force was quantified by a portable sensor measurement system (compression piezoelectric sensor (CL-YD-303) integrated with a four-channel dynamic signal acquisition module (NI 9234) and compact data acquisition chassis (NI, cDAQ-9171)).
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5

Upconversion Emission Lifetime and RET Efficiency

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For the measurements of upconversion emission lifetime, the beam at the wavelength of 980 nm from a CW laser (Thorlabs, BL976-PAG900) was modulated using an acousto-optic modulator (AOM) (AA OPTO-ELECTRONIC, MT110-A1-VIS/IR/1064) for 50-μs pulses with a frequency of 100 Hz for excitation of upconversion emission. The emitted photons went through a bandpass filter (Semrock, FF01-442/46-25) and a short pass filter (Semrock, FF02-694/SP-25) and were detected by a SPAD (Excelitas Technologies, SPCM-AQRH-14-FC). The trigger signal from the AOM was synchronized with the SPAD using a data acquisition (DAQ) card (National Instruments, cDAQ-9171). The effective emission decay time, τeff, was calculated by τeff=1I00I(t)dt where I(t) denotes the emission intensity as a function of time, t and I0 represents the maximum emission intensity. The RET efficiency, ERET, was derived from the upconversion emission lifetime measurements and calculated as ERET=1τDAτD where τDA and τD are the lifetime of the donor (UCNPs) in the presence and absence of the acceptor (GO), respectively.
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6

Continuous Blood Pressure Monitoring Methods

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This study simultaneously employed two continuous BP measurement systems (Figure 1A). The A-Line was inserted into the radial artery and connected to a pressure transducer [ICU Medical Transpace© IV Monitoring Kit (60″), REF no. 42584-05] and displayed on a monitoring system (GE Patient Data Module & Monitoring system, General Electric, Boston, MA). The A-Line signal was then captured at an average sampling rate of 100 Hz using a DAQ board (National Instruments cDAQ-9171 with NI 9234) with a custom application written in C#.
The non-invasive system comprises a soft capacitive pressure sensor (CAP) (18 (link)) and an EcoBP (19 ), an eco-friendly dual-channel custom data acquisition board that includes an inertial measurement unit (IMU). The CAP sensor was placed at the radial artery (or in two cases, the dorsalis pedis artery) for continuous arterial pressure measurement. The CAP signal was captured at a sampling rate of 90 Hz in single-channel mode and 45 Hz in dual-channel mode.
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7

Cantilever-based Tissue Indentation

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A custom cantilever-based indenter (Fig 1) [16 (link)] was used to indent tissue samples and record force relaxation over time. A piezoelectric stage (P-628.1CD, Physik Instrumente) displaced a soft titanium cantilever with a 4 mm-diameter rigid tip. Cantilever stiffness (79.8 N/m) was calibrated directly with small weights hung from the cantilever tip. A custom program in LabVIEW (National Instruments) was used to control indentation profile and to read deflection of cantilever tip with capacitive sensor (C8S-3.2–2.0 and compact driver CD1-CD6, Lion Precision) through a data acquisition card system (NI 9220 and cDAQ-9171, National Instruments). The cantilever base was driven to 10% of the total thickness of the samples at 15 μm/s. The cantilever was then held as this position while the tissue underwent stress relaxation. Stress relaxation times varied between 60–180 s to allow the various samples to reach a quasi-static state. Samples were kept hydrated in between indentations by submerging them in 1x DMEM cell media (Dow Corning).
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8

Measuring Vibration Velocity with Doppler Laser

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To measure the velocity of the vibrations applied and measured, we used a Doppler laser vibrometer (PDV‐100, Polytec Ltd, Coventry, UK) set to 500 mm/s maximum velocity and a Low Pass Filter at 22 kHz. The force applied by the shaker was simultaneously measured using the miniature force sensor. The signals of both the laser vibrometer and the force sensor were simultaneously acquired using a two‐channel NI9250 Sound and Vibration module (NI Corporation [UK] Ltd, Newbury, UK) and a USB‐powered data acquisition module (cDAQ‐9171, NI). The acquisition was done using custom‐written software in LabView NXG 5.1 (NI). Samples were acquired at a rate of 10,240 samples per second. Data were saved in TDMS format and subsequently converted to text files using a custom program in LabView.
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9

EEG/EMG Signal Acquisition and Processing

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The EEG/EMG signals were amplified (gain ×1000) and filtered (EEG:1–300 Hz, EMG:10–300 Hz) using a DC/AC differential amplifier (AM-3000, AM systems, Sequim, WA, USA). The input was then received via an input module (NI-9215, National Instruments, Austin, TX, USA), digitized at a sampling rate of 1000 Hz using a data acquisition module (cDAQ-9171, National Instruments, Austin, TX, USA), and recorded using a custom-made LabVIEW program (National Instruments, Austin, TX, USA). We habituated the 24-hour EEG/EMG recordings more than three times; when REM sleep (see vigilance state assessment) was consistently observed, the experiment was initiated.
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10

Ginkgo biloba Dripping Pill Formulation and Characterization

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The dripping experiment was conducted using a formulation containing Ginkgo biloba leaf extract (Zhejiang Conba Pharmaceutical Co., Ltd., Hangzhou, China) and polyethylene glycol 4000 (Wanbangde Pharmaceutical Group, Wenling, China). The dimethyl silicone oil was used as condensing oil (Jiangxi Alpha Hi-tech Pharmaceutical Co., Ltd., Pingxiang, China). The petroleum ether was used as condensing oil washing solvent (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China). The authors confirm that the present study complies with the IUCN Policy Statement on Research Involving Species at Risk of Extinction and the Convention on the Trade in Endangered Species of Wild Fauna and Flora.
The weight of the dripping pills was in-line measured using a laser detection system which is equipped with a laser micrometer (KEYENCE IG-028, Shanghai, China), a sensor amplifier (KEYENCE IG-1000, Shanghai, China), and a data acquisition card (National Instrument cDAQ-9171, Shanghai, China). The weight of materials was weighed using an electric balance (Mettler Toledo AE240, Shanghai, China). The homogenous dispersing liquid was prepared using a circulating oil bath (Greatwall Scientific SY-20, Zhengzhou, China) and an electric mixer (Zhengrong instrument ES-60 M, Changzhou, China). The preparation of dripping pills was performed using a multifunction dripping machine (Anruikang, Beijing, China).
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