Cdaq 9171

This study simultaneously employed two continuous BP measurement systems (Figure 1A). The A-Line was inserted into the radial artery and connected to a pressure transducer [ICU Medical Transpace^© IV Monitoring Kit (60″), REF no. 42584-05] and displayed on a monitoring system (GE Patient Data Module & Monitoring system, General Electric, Boston, MA). The A-Line signal was then captured at an average sampling rate of 100 Hz using a DAQ board (National Instruments cDAQ-9171 with NI 9234) with a custom application written in C#.
The non-invasive system comprises a soft capacitive pressure sensor (CAP) (18 (link)) and an EcoBP (19 ), an eco-friendly dual-channel custom data acquisition board that includes an inertial measurement unit (IMU). The CAP sensor was placed at the radial artery (or in two cases, the dorsalis pedis artery) for continuous arterial pressure measurement. The CAP signal was captured at a sampling rate of 90 Hz in single-channel mode and 45 Hz in dual-channel mode.

A custom cantilever-based indenter (Fig 1) [16 (link)] was used to indent tissue samples and record force relaxation over time. A piezoelectric stage (P-628.1CD, Physik Instrumente) displaced a soft titanium cantilever with a 4 mm-diameter rigid tip. Cantilever stiffness (79.8 N/m) was calibrated directly with small weights hung from the cantilever tip. A custom program in LabVIEW (National Instruments) was used to control indentation profile and to read deflection of cantilever tip with capacitive sensor (C8S-3.2–2.0 and compact driver CD1-CD6, Lion Precision) through a data acquisition card system (NI 9220 and cDAQ-9171, National Instruments). The cantilever base was driven to 10% of the total thickness of the samples at 15 μm/s. The cantilever was then held as this position while the tissue underwent stress relaxation. Stress relaxation times varied between 60–180 s to allow the various samples to reach a quasi-static state. Samples were kept hydrated in between indentations by submerging them in 1x DMEM cell media (Dow Corning).

The EEG/EMG signals were amplified (gain ×1000) and filtered (EEG:1–300 Hz, EMG:10–300 Hz) using a DC/AC differential amplifier (AM-3000, AM systems, Sequim, WA, USA). The input was then received via an input module (NI-9215, National Instruments, Austin, TX, USA), digitized at a sampling rate of 1000 Hz using a data acquisition module (cDAQ-9171, National Instruments, Austin, TX, USA), and recorded using a custom-made LabVIEW program (National Instruments, Austin, TX, USA). We habituated the 24-hour EEG/EMG recordings more than three times; when REM sleep (see vigilance state assessment) was consistently observed, the experiment was initiated.

The dripping experiment was conducted using a formulation containing Ginkgo biloba leaf extract (Zhejiang Conba Pharmaceutical Co., Ltd., Hangzhou, China) and polyethylene glycol 4000 (Wanbangde Pharmaceutical Group, Wenling, China). The dimethyl silicone oil was used as condensing oil (Jiangxi Alpha Hi-tech Pharmaceutical Co., Ltd., Pingxiang, China). The petroleum ether was used as condensing oil washing solvent (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China). The authors confirm that the present study complies with the IUCN Policy Statement on Research Involving Species at Risk of Extinction and the Convention on the Trade in Endangered Species of Wild Fauna and Flora.
The weight of the dripping pills was in-line measured using a laser detection system which is equipped with a laser micrometer (KEYENCE IG-028, Shanghai, China), a sensor amplifier (KEYENCE IG-1000, Shanghai, China), and a data acquisition card (National Instrument cDAQ-9171, Shanghai, China). The weight of materials was weighed using an electric balance (Mettler Toledo AE240, Shanghai, China). The homogenous dispersing liquid was prepared using a circulating oil bath (Greatwall Scientific SY-20, Zhengzhou, China) and an electric mixer (Zhengrong instrument ES-60 M, Changzhou, China). The preparation of dripping pills was performed using a multifunction dripping machine (Anruikang, Beijing, China).