Rbc lysis

Manufactured by Merck Group

Sourced in United States

RBC lysis is a lab equipment product that facilitates the disruption and breakdown of red blood cells (erythrocytes). It is designed to separate cellular components, allowing for the isolation and analysis of specific cell types or molecules within a sample.

Automatically generated - may contain errors

Lab products found in correlation

Pan b magnetic beads, by Miltenyi Biotec (2 mentions) Mouse tumor dissociation kit, by Miltenyi Biotec (1 mentions) Myeloid derived suppressor cell isolation kit, by Miltenyi Biotec (1 mentions) Celltrace cfse, by Thermo Fisher Scientific (1 mentions) Mhcii alexa 700, by Thermo Fisher Scientific (1 mentions) Cd43 ly 48 microbeads, by Miltenyi Biotec (1 mentions) Facsaria 2 sorp, by BD (1 mentions) Trustain αcd16 cd32 fc block, by BioLegend (1 mentions)

Spelling variants of this product

RBC lysis buffer (280 citations) RBC lysis solution (9 citations) Hybri-Max RBC lysis buffer (4 citations) RBC blood lysis buffer (4 citations) RBCs lysis buffer (3 citations) RBC (red blood cell) lysis buffer (3 citations) Hypotonic ammonium chloride RBC lysis buffer (2 citations)

6 protocols using rbc lysis

Mouse Immune Cell Isolation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Splenocytes were obtained from mice by mashing the spleens through cell strainers followed by RBC lysis (Sigma). Mouse B cells and CLL cells were purified from mouse spleens by negative selection using CD43 (Ly48) or Pan-B magnetic beads (Miltenyi Biotech), respectively, following the manufacturer’s instructions. MDSCs were purified from spleens, bone marrow or LLC tumors (digested with the mouse tumor dissociation kit purchased from Miltenyi Biotech) by positive selection using myeloid-derived suppressor cell isolation kit (Miltenyi Biotech).

Tang C.H., Chang S., Hashimoto A., Chen Y.J., Kang C.W., Mato A.R., Del Valle J.R., Gabrilovich D.I, & Hu C.C. (2018). Secretory IgM exacerbates tumor progression by inducing accumulations of MDSCs in mice. Cancer immunology research, 6(6), 696-710.

+ Open protocol

+ Expand

OT-II T Cell Proliferation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

OT-II/CD45.1 mice were euthanized and their spleens and lymph nodes collected. These were disrupted and after RBC lysis (Sigma-Aldrich, St. Louis, MO) CD4⁺ T cells were isolated by negative selection using the mouse CD4⁺ T cell isolation kit 19852A (Stemcell Technologies; Seattle, WA) and labeled with Celltrace CFSE (Invitrogen; Eugene, OR). 1 × 10⁷ CFSE-labeled T cells were injected into the tail vein of 77His or WT mice (day 0), and 24 h later (day 1), each mouse received i.v. 100 μg OVA (A-5503; Sigma Aldridge). On day 4, mice were euthanized and their spleens were harvested. Spleen cells were isolated and labeled with anti-CD4 and anti-CD45.1 antibodies, and CD4⁺CD45.1⁺CFSE⁺ OT-II T cells (events) identified by flow cytometry. To determine the percentage of T cells present in each proliferating generation, the following equation was used:

\frac{Events in Generation \times 2^{Generation Number}}{\sum Transformed Events}

Avery J.T., Jimenez R.V., Blake J.L., Wright T.T., Leόn-Ruiz B., Schoeb T.R., Szalai A.J, & Bullard D.C. (2019). Mice expressing the variant rs1143679 allele of ITGAM (CD11b) show impaired DC-mediated T cell proliferation. Mammalian Genome, 30(9), 245-259.

+ Open protocol

+ Expand

Flow Cytometric Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single cell suspensions from harvested tissues were prepared for flow cytometry as previously described (23 (link)). After red blood cell (RBC) lysis (Sigma), single-cell suspensions were incubated for 30 minutes on ice with the following antibodies: CD45-APC, CD11b-APC or CD11b-e450, Gr-1-PerCPCy5.5, Ly6C-FITC, F4/80-PE-Cy7 or F4/80-e450, MHCII-Alexa-700, and CD115 (CSF1R)-PE conjugated antibodies, 1:200 (eBioscience), followed by two washes with 2% FBS in PBS (FACS buffer). Cells were fixed in 3% PFA for 15 min at room temperature and washed two times with FACS buffer. Cell acquisition was done on a BD LSR-II flow cytometer (Beckman Coulter). Data were analyzed with FlowJo software (TreeStar) (23 (link)).

Escamilla J., Schokrpur S., Liu C., Priceman S.J., Moughon D., Jiang Z., Pouliot F., Magyar C., Sung J.L., Xu J., Deng G., West B.L., Bollag G., Fradet Y., Lacombe L., Jung M.E., Huang J, & Wu L. (2015). CSF1 Receptor Targeting In Prostate Cancer Reverses Macrophage-Mediated Resistance To Androgen Blockade Therapy. Cancer research, 75(6), 950-962.

+ Open protocol

+ Expand

Isolation and Activation of CD43- Splenic B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

CD43⁻ splenic B cells were isolated subsequent to RBC lysis (Sigma) by negative selection using CD43 (Ly-48) Microbeads (Miltenyi), and cultured with α-CD40+IL4, LPS+α-IgD and RP105 as described (3 (link)).

Park J., Welner R.S., Chan M.Y., Troppito L., Staber P.B., Tenen D.G, & Yan C.T. (2015). The DNA ligase IV syndrome R278H mutation impairs B-lymphopoiesis via error-prone non-homologous end-joining. Journal of immunology (Baltimore, Md. : 1950), 196(1), 244-255.

+ Open protocol

+ Expand

Isolation of Mouse Splenocytes and B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Splenocytes were obtained from mice by mashing the spleens through cell strainers followed by RBC lysis (Sigma-Aldrich). Mouse B cells were purified from mouse spleens by negative selection using CD43 (Ly48) or pan-B magnetic beads (Miltenyi Biotec), according to the manufacturer’s instructions.

Tang C.H., Chang S., Paton A.W., Paton J.C., Gabrilovich D.I., Ploegh H.L., Del Valle J.R, & Hu C.C. (2018). Phosphorylation of IRE1 at S729 regulates RIDD in B cells and antibody production after immunization. The Journal of Cell Biology, 217(5), 1739-1755.

+ Open protocol

+ Expand

Isolation and Activation of Lung CD4+ TRM Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single-cell suspensions prepared from digested lungs were prepared with RBC lysis (Sigma-Aldrich, St. Louis, MO, USA) and blocked with TruStain αCD16/CD32 Fc-Block (BioLegend, San Diego, CA, USA) for 5 minutes. Cells were stained in FACS buffer with optimized dilutions of antibodies. GL7⁺ and GL7⁻ subsets of lung (i.v.CD45.2⁻) CD4⁺ T_RM cells were sorted into 20% FBS RPMI 1640 on ice with FACS-Aria II SORP (BD Biosciences, Franklin Lakes, NJ, USA). After sorting, cells were cultured for 6 hours with T cell media with or without PMA/Ionomycin stimulation at 37°C and 5% CO₂. Immediately after, cells were washed and pelleted into (RNA) ribonucleic acid Lysis Buffer (Qiagen, Germantown, MD, USA) and stored at −80°C until RNA extraction.

Kajino T., Takahashi H., Hirai M, & Yamada Y. (2000). Efficient Production of Artificially Designed Gelatins with a Bacillus brevis System. Applied and Environmental Microbiology, 66(1), 304-309.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!