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Rna bioanalyzer kit

Manufactured by Agilent Technologies
Sourced in United States

The RNA Bioanalyzer kit is a laboratory instrument designed for the analysis and quality assessment of RNA samples. It provides detailed information about the integrity and concentration of RNA molecules in a sample through automated electrophoretic separation and detection.

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2 protocols using rna bioanalyzer kit

1

Comprehensive RNA Extraction and miRNA Expression Profiling

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Total RNA was extracted using TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.) and purified using RNA Cleanup and Concentration kit according to the manufacturer's protocol (Norgen Biotek Corp.). RNA quantification was performed using Nanodrop 2000 at 260 nm wavelength (Thermo Fisher Scientific, Inc.) and RNA integrity and purity was assessed with RNA Bioanalyzer kit according to the manufacturer's protocol (Agilent Technologies, Inc.). miR expression profile was performed using the nCounter Human v2 miRNA Expression Assay and nCounter Nanostring platform according to manufacturer's protocol (NanoString Technologies).
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2

RNA Extraction and Quality Assessment

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Total RNA was extracted using TRIzol (Invitrogen, Carlsbad, CA, USA) and purified using RNA Cleanup and Concentration kit (Norgen, Thorold, ON, Canada). RNA quantification was performed using Nanodrop 2000 (Thermo-Scientific, Waltham, MA, USA) and RNA integrity and purity was assessed with RNA Bioanalyzer kit (Agilent Technologies, Santa Clara, CA, USA) considering samples with an RNA Integrity Number (RIN) at least of 7.
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