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2 protocols using anti phosphorylated and total flt3

1

Antibody and Reagent Profiling for CML

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Anti-phosphorylated and total FLT3, AKT, BTK, STAT5 and MAPK antibodies were purchased from Cell Signalling Technology (Cambridge, MA). Anti-CD34-PE, anti-CD90-FITC, anti-CD73-PE, anti-CD105-APC antibodies were purchased from Miltenyi Biotec (Auburn, CA, USA). Ibrutinib was obtained from Selleck Chemicals. All other reagents were obtained from Sigma-Aldrich (St Louis, MO, USA), unless indicated.
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2

Protein Expression and Apoptosis Assays

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Anti-phosphorylated and total FLT3, BTK (223), AKT, STAT5 and ERK, CCDN2, CCND3, CDK2, CDK4, CDK6, P53, γH2AX, P21WAF1/CIP1, CASPASE-3, CASPASE-7, CASPASE-8, PARP, Bad, Bax, Bcl-2, Bcl-XL, MCL-1, Pim-2, γ-H2AX, and β-actin antibodies were purchased from Cell Signalling Technology (Beverly, MA, USA). Pim-1 was obtained from Abcam (Cambridge, MA). Ibrutinib was obtained from Selleck Chemicals (Houston, USA). HHT was purchased from Sigma-Aldrich (St. Louis, MO, USA).
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