Mice were bred and maintained in the specific pathogen-free (SPF) animal facility at UT MD Anderson Cancer Center in accordance with institutional guidelines. We generated RORγf/f mice, in which exon 2 and 3 of Rorc gene are flanked by LoxP sites. The resultant RORγf/f mice were bred to FLPeR mice to remove Neomycin resistance cassette and then back-crossed to C57BL/6 mice for six generations. Foxp3YFP-Cre knock-in mice and Foxp3GFP reporter mice were kindly provided by Dr. Alexander Rudensky (Memorial Sloan-Kettering Cancer Center). Foxp3GFP-Cre BAC transgenic mice were kindly provided by Drs. Jeffrey Bluestone (UCSF) and Shao-Cong Sun (UT MD Anderson Cancer Center). Stat3f/fCD4Cre and B7h−/− mice were previously described (Nurieva et al., 2003 (link); Yang et al., 2007 (link)). C57BL/6 and BALB/c mice were purchased from Jackson Laboratories. Foxp3GFP reporter mice on BALB/c background and DO11.10 mice were obtained from Jackson Laboratories and were crossed in our animal facility. Female and male mice at 8–12 weeks of age were used for experiments.
Do11.10 mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
DO11.10 mice are a transgenic mouse strain that expresses a T cell receptor (TCR) specific for a peptide from chicken ovalbumin (OVA). These mice are widely used in immunological research to study T cell activation and response to antigen presentation.
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Lab products found in correlation
Balb c mice, by Jackson ImmunoResearch (2 mentions)
C57bl 6 and balb c mice, by Jackson ImmunoResearch (1 mentions)
Ot 2, by Jackson ImmunoResearch (1 mentions)
C57bl 6 mice, by Jackson ImmunoResearch (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
L glutamine, by Merck Group (1 mentions)
2 mercaptoethanol, by Merck Group (1 mentions)
Balb c mice, by Charles River Laboratories (1 mentions)
Ot 2 mice, by Jackson ImmunoResearch (1 mentions)
Nos2 mice, by Jackson ImmunoResearch (1 mentions)
C3h hej, by Jackson ImmunoResearch (1 mentions)
Wild type balb c and c57bl 6 mice, by Jackson ImmunoResearch (1 mentions)
C57bl 6, by Charles River Laboratories (1 mentions)
Balb c, by Charles River Laboratories (1 mentions)
C57bl 6, by Jackson ImmunoResearch (1 mentions)
12 protocols using do11.10 mice
1
Generation and Characterization of Genetically Modified Mice
2
Murine Model of Gamma-delta T Cell Activation
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4–8 week old female OT-II and DO11.10 mice were obtained from Jackson Lab (Bar Harbor, ME). Thy1.1 IL-17F reporter mice, originally developed in the lab of Casey Weaver at University of Alabama Birmingham47 (link), were bred in-house at The University of Pittsburgh. All animal studies were approved by the Institutional Animal Care and Use Committee (IACUC) of the University of Pittsburgh. All experiments were performed in accordance with IACUC guidelines and regulations. 8–10 week old female C57BL/6 mice from Jackson lab were used for in vivo studies. On day 0 they were given 5mg/kg Compound B) by oral gavage or same volume of vehicle control. Compound B or vehicle control was dosed every 12–18 hours for 3 doses on day 0 and day 1. On day 1, 24 hours before sacrificing, every mouse received Intranasal IL-23 (500 ng/mouse) and IL-1β (25 ng/mouse) to induce gamma-delta T cell activation35 (link)36 (link). On Day 2 the mice were sacrificed, BAL was performed, and lungs were harvested. All animal studies were approved by the Institutional Animal Care and Use Committee of the University of Pittsburgh.
3
Culturing Murine Immune Cells
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BALB/c mice were purchased from Charles River Laboratories; DO11.10 mice (on the BALB/c background) were derived from The Jackson Laboratory. Both strains were bred and maintained under specific pathogen free conditions in the animal unit of the Eötvös Loránd University. Mice were used at 6–18 wk of age. Spleen or lymph node cell suspensions were cultured in RPMI 1640 medium (GIBCO, Invitrogen, Carlsbad, CA, US) supplemented with 5% heat-inactivated FCS (GIBCO), 2 mM L-glutamine (Sigma-Aldrich, St. Louis, MO, US), 100 U/mL penicillin (Sigma-Aldrich), 100 µg/mL streptomycin (Sigma-Aldrich), 50 µM 2-mercaptoethanol (Sigma-Aldrich), and 1 mM sodium pyruvate (Reanal, Budapest, HU).
4
Mouse Strains for Immunology Research
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BALB/c and C57BL/6 (B6) mice were obtained from Harlan UK. Nos2−/− mice, DO11.10 mice (BALB/c background) and OT-II mice (B6 background) were originally from the Jackson Laboratory. Irf4−/− mice (B6 background) were as described previously56 (link). Cells from the p53−/− mice (B6 background) were provided by Dr. Ewen McGregor (University of Glasgow). Cells from the Stat5−/− mice (B6 background) were provided by Dr. Arian Laurence (NIH, Bethesda). Nos2−/− mice (B6 background) were obtained from the Jackson laboratory. Male and female mice were used at the age of 6-10 weeks. Cells from BALB/c and C57BL/6 mice gave similar results. All animal experiments were performed according to institutional guidelines in the Ribeirão Preto Medical School, University of São Paulo, Brazil (USP Number. 038/2009) and in accordance with the French ethical and animal experiments regulations in the project approved by the Ethics Committee for Animal Experimentation of CNRS Campus Orleans (N° CLE CCO 2012-050, UMR7355, Orleans, France).
5
Mouse Strain Acquisition and Maintenance
6
Evaluating Immune Responses in Mice
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BALB/c and Ova-TCR transgenic DO11.10 mice (BALB/c background, Jackson Laboratories, Bar Harbor, Maine, USA), C57BL/6 mice (Harlan, Rossdorf, Germany), IL-10−/− mice (BALB/c background), Ova-TCR transgenic OT-II and TLR5−/−, MyD88−/−, Trif−/−, MyD88−/−Trif−/− mice (all C57BL/6 background) were bred at the animal facility of the Paul-Ehrlich-Institut and kept under specified pathogen free conditions.
7
Mouse Strain Acquisition and Approval
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Wild-type BALB/c and C57BL/6 mice and DO11.10
mice were purchased from the Jackson Laboratory. All animal experiments
were approved by the Institutional Animal Care and Use Committee for
UC San Diego.
mice were purchased from the Jackson Laboratory. All animal experiments
were approved by the Institutional Animal Care and Use Committee for
UC San Diego.
8
OVA-specific TCR-transgenic Mouse Protocol
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Ovalbumin (OVA)-specific TCR-transgenic DO11.10 mice were obtained from the Jackson Laboratory (Bar Harbor, ME, USA). Their offspring were used at 5–7 weeks of age. The T cells of these mice recognize OVA 323–339 restricted to I-Ad. The TCR-transgenic mice were bred in the animal facility of our university and were maintained on irradiated food and autoclaved distilled water. All mice were maintained and used in accordance with the guidelines for the care and use of experimental animals of Tokyo University of Agriculture and Technology. The procedures of this study were approved by the animal care and use committee in Tokyo University of Agriculture and Technology (26–8, April 1st, 2014; 27–3, April 17th, 2015; 28–3, April 19th, 2016; 30–6, April 6th, 2018). We checked the mice almost every day and confirmed no adverse clinical signs through the experimental periods. Splenocytes were prepared from the mice immediately after sacrificing them by cervical dislocation.
9
DO11-10-tg Rag-2 BALB/c Mice Protocol
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All mice used in this study were 8-week-old male mice. BALBc mice were from Jackson Lab (Bar Harbor, ME). DO11-10-tg Rag-2-/- BALB/c mice were from our in house-breeding colony housed at the University of Florida as previously described.7 (link),28 (link),35 (link) These mice are immune deficient, while their CD4+ T cells exclusively express the DO11.10 TCR specific for the model antigen ovalbumin. Immune-competent DO11.10 mice for the adoptive transfer experiments were purchased from Jackson Lab. All experiments were performed with a minimum of n = 3 animals per group.
10
BALB/c and DO11.10 Mice Maintenance
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BALB/c mice were purchased from Guangdong Experimental Animal Center (Guangzhou, China). DO11.10 mice were purchased from Jackson Laboratory (Bar Harbor, Maine). Mice were maintained in a specific pathogen-free facility at with accessing water and food freely. The animal experimental procedures were approved by the Animal Ethics Committee at Shenzhen University.
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