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Dmi inverted fluorescent microscope

Manufactured by Leica
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The DMI inverted fluorescent microscope is a versatile imaging instrument designed to visualize fluorescently-labeled samples. It features a modular and configurable optical system that allows for a range of imaging techniques, including phase contrast, differential interference contrast (DIC), and epifluorescence. The DMI microscope is optimized for live-cell and high-resolution imaging applications.

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Fluoview confocal microscope, by Olympus (1 mentions)

Close spelling variants of this product

Inverted microscope (717 citations) Fluorescent microscope (451 citations) Inverted fluorescent microscope (69 citations) Leica DMI fluorescent microscope (2 citations)

5 protocols using dmi inverted fluorescent microscope

1

Quantification of Pancreatic Beta Cells

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Freshly isolated pancreases were placed in microfuge tubes, snap-frozen in liquid nitrogen, and stored at −70 °C until use. Every 10th section of 10 μm thickness was collected and fixed in 4% paraformaldehyde and 1% Triton-X 100 in PBS. Pancreas sections were washed, blocked for 20 min in 2% BSA in PBS, and incubated for 30 min each with primary and secondary antibodies, insulin rabbit mAb and biotinylated secondary antibody, respectively. Following the manufacturer's instructions, tissues were stained with AB enzyme reagent, followed by peroxidase substrate containing DAB chromagen. Tissues were counterstained with Gill's formulation #2 hematoxylin and mounted on microscope slides. Images of pancreas sections were captured using a 2.5X objective in a Leica DMI inverted fluorescent microscope. The ratio of β-cell area to exocrine tissue area per section was calculated using the ImageJ image processing and analysis program.
Esmaeili Mohsen Abadi S., Balouchzadeh R., Uzun G., Ko H.S., Lee H.F., Park S, & Kwon G. (2020). Tracking changes of the parameters of glucose-insulin homeostasis during the course of obesity in B6D2F1 mice. Heliyon, 6(1), e03251.
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2

Isolated Rat Islet Glucose Regulation

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Isolated rat islets (30 (link)) were incubated for 2 days in complete CMRL-1066 containing 10 mM glucose in the presence and absence of 10 μM SR-135 or 10 μM SRB. Color and fluorescent images of islets were captured using a 20X objective in a Leica DMI inverted fluorescent microscope.
Johns M., Fyalka R., Shea J.A., Neumann W.L., Rausaria S., Msengi E.N., Imani-Nejad M., Zollars H., McPherson T., Schober J., Wooten J, & Kwon G. (2015). SR-135, a Peroxynitrite Decomposing Catalyst, Enhances β-cell Function and Survival in B6D2F1 Mice Fed a High Fat Diet. Archives of biochemistry and biophysics, 0, 49-59.
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3

Immunohistochemical Analysis of Pancreatic β-Cells

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Pancreas sections were washed, blocked for 20 min in 2% BSA in PBS, and incubated for 30 min each with primary and secondary antibodies, insulin rabbit mAb and biotinylated secondary antibody, respectively. Following the manufacturer’s instructions, tissues were stained with AB enzyme reagent, followed by peroxidase substrate containing DAB chromagen. Tissues were counterstained with Gill’s formulation #2 hematoxylin and mounted on microscope slides. Images of pancreas sections were captured using a 2.5X objective in a Leica DMI inverted fluorescent microscope. The ratio of β-cell area to exocrine tissue area per section was calculated using the ImageJ image processing and analysis program.
Johns M., Fyalka R., Shea J.A., Neumann W.L., Rausaria S., Msengi E.N., Imani-Nejad M., Zollars H., McPherson T., Schober J., Wooten J, & Kwon G. (2015). SR-135, a Peroxynitrite Decomposing Catalyst, Enhances β-cell Function and Survival in B6D2F1 Mice Fed a High Fat Diet. Archives of biochemistry and biophysics, 0, 49-59.
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4

Histological Analysis of Liver Glycogen

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Freshly isolated right lobe of the liver was snap-frozen in liquid nitrogen and stored at -70°C until use. Frozen section of 10 μm thickness was collected and fixed in 4% paraformaldehyde and 1% Triton-X 100 in PBS. Liver sections were washed in PBS to remove the residual paraformaldehyde and divided into two conditions with and without treatment with freshly prepared 0.5% alpha-amylase for 20 min. Liver sections were incubated with 0.5% periodic acid solution for 5 min, then stained with Schiff’s reagent for 15 min. All steps were performed at room temperature, and liver sections were rinsed with distilled water after each step. Color images of liver sections were captured using a 20X objective in a Leica DMI inverted fluorescent microscope using ISCapture Software.
Kirilmaz O.B., Salegaonkar A.R., Shiau J., Uzun G., Ko H.S., Lee H.F., Park S, & Kwon G. (2021). Study of blood glucose and insulin infusion rate in real-time in diabetic rats using an artificial pancreas system. PLoS ONE, 16(7), e0254718.
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5

Hydrogel Cryosectioning and Immunostaining

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After retrieval, each hydrogel was cut into 8 pieces and placed in a microfuge tube containing 800 μl of Bright Cryo-M-Bed freezing medium. Hydrogels in microfuge tubes were snap-frozen in liquid nitrogen, and stored at −70°C until use. Frozen hydrogel sections of 10 μm thickness were cut using a Vibratome (St. Louis, MO) and every 10th section was transferred to a coverslip. Hydrogel sections were placed in Wheaton staining jars filled with de-ionized (DI) water and left to soak for 5 min. The hydrogel sections were stained with Hematoxylin solution for 1 min, rinsed with water, and mounted on microscope slides. Images of hydrogel sections were captured using a 5X objective in a Leica DMI inverted fluorescent microscope. For immunohistochemistry, hydrogel sections were washed, blocked, and immunostained with Alexa fluor 647 anti-mouse F4/80 antibody for murine macrophages and DAPI for nuclear staining. Fluorescent images were obtained using a 40X objective in an Olympus FluoView confocal microscope (Olympus Corporation, Waltham, Massachusetts).
Knobeloch T., Abadi S.E., Bruns J., Zustiak S.P, & Kwon G. (2017). Injectable Polyethylene Glycol Hydrogel for Islet Encapsulation: an in vitro and in vivo Characterization. Biomedical physics & engineering express, 3, 035022.
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