MDZ was purchased from Yichang Renfu Pharmacy Company (Yichang, China). Diazepam was purchased from Shandong Xinyi Pharmaceutical Co., Ltd. (Dezhou, China). Poloxamer 407 (P407), poloxamer 188 (P188), Macrogol 4000 (PVP K30), and Sodium dodecyl sulfate (SDS) were purchased from BASF, Ludwigshafen, Germany. Polysorbate 80 (TW80), polysorbate 20 (TW20), and carboxymethylcellulose sodium (CMC-Na) were ordered from Sinopharm Chemical Reagent Co. (Beijing, China). Sodium dodecyl sulfate was procured from VWR International (Radnor, PA, USA). Hydroxypropyl methylcellulose (HPMC) was purchased from Dow Chemical Company (Michigan, MI, USA). Dioctyl sodium sulfosuccinate (DOSS) was purchased from Hunan HuaNa Pharmaceutical Factory Chiral Pharmaceutical Co., Ltd. (Hunan, People’s Republic of China). HPLC-grade acetonitrile and methanol were obtained from Thermo Fisher Scientific (Waltham, MA, USA). All other reagents were of analytical grade. The purified water used in this study was prepared using a Mille-Q system (EMD Millipore, Billerica, MA, USA). The Cu/Zn-SOD assay kit with WST-8, the GSH assay kit, the lipid peroxidation MDA assay kit, and the BCA protein assay kit were purchased from Shanghai Biyuntian Biotechnology Co., Ltd. (Shanghai, China).
Mille q system
Manufactured by Merck Group
Sourced in United States
The Mille-Q system is a water purification system designed to produce ultrapure water. It utilizes a combination of technologies, including reverse osmosis and ion exchange, to remove impurities and contaminants from the water. The Mille-Q system is capable of producing water with a high degree of purity, making it suitable for a variety of laboratory and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Thermo Fisher Scientific (4 mentions)
Acetonitrile, by Thermo Fisher Scientific (4 mentions)
Sodium dodecyl sulfate (sds), by BASF (3 mentions)
Hplc grade acetonitrile, by Thermo Fisher Scientific (2 mentions)
Hplc grade triethylamine, by Thermo Fisher Scientific (2 mentions)
Ripa buffer, by Thermo Fisher Scientific (2 mentions)
Sodium dodecyl sulfate (sds), by Avantor (1 mentions)
Sodium carboxymethyl cellulose cmc na, by Sinopharm (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Hydroxypropyl methylcellulose hpmc, by Dow (1 mentions)
Phenyl methyl sulfonyl fluoride (pmsf), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Polyvinyl alcohol (pva), by Kuraray (1 mentions)
Plga 75 25, by Evonik (1 mentions)
5 protocols using mille q system
1
Reagents for Oxidative Stress Assays
2
Synthesis and Formulation of GeXIVA
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Synthesis of GeXIVA[1,2] performed as previously described [24 (link)]. Trehalose was in injection-grade and purchased from Hayashibara Co., Ltd. (Okayama, Japan). Injection-grade glucose, mannitol, sorbitol, and glycine were purchased from Beijing Fengli Jingqiu Pharmaceutical Co., Ltd. (Beijing, China). Polyethylene glycol 2000 (PEG 2000) was purchased from BASF (Ludwigshafen, Germany). Paclitaxel was provided by Zhejiang Hisun Pharmaceutical Co., Ltd. (Taizhou, China). HPLC-grade triethylamine and acetonitrile were obtained from Thermo Fisher Scientific (Waltham, MA, USA). The purified water used in this study was prepared using a Mille-Q system (EMD Millipore, Billerica, MA, USA).
3
Optimizing Drug Delivery Formulations
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CsA was purchased from Taishang Chemical Pharmacy Company (Taishang, China). Cyclosporin D (CsD) was purchased from Shanghai Tongtian Biotechnology Company (Shanghai, China). Vitamin E polyethylene glycol succinate (TPGS) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Sodium dodecyl sulfate (SDS) was purchased from BASF, Ludwigshafen, Germany. Hydroxypropyl cellulose (HPC) was purchased from Nippon Soda Co., Ltd. (Tokyo, Japan). Roswell Park Memorial Institute (RPMI) 1640 medium, phosphate buffered saline (PBS) and Hank’s Balanced Salt Solution (HBSS) were purchased from HyClone Co., Ltd. (Logan, UT, USA). Fetal bovine serum (FBS) was purchased from gibco Co., Ltd. (GrandIsland, NY, USA). PMSF and RIPA buffer were purchased from Thermo Fisher Scientific Inc. (Rochester, NY, USA). The purified water used in this study was prepared using a Mille-Q system (EMD Millipore, Billerica, MA, USA). HPLC-grade acetonitrile and methanol were obtained from Thermo Fisher Scientific (Waltham, MA, USA). All other reagents were analytical grade.
4
Formulation and Characterization of Trehalose-Loaded PLGA Nanoparticles
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GeXIVA[1,2] API was provided by Gill Biochemical Co., Ltd. (Shanghai). PLGA 75/25 was purchased from EVONIK. Trehalose was injection-grade and was purchased from Hayashihara Co., Ltd. (Okayama, Japan). Polyvinyl alcohol (PVA) was purchased from Kuraray co., ltd. HPLC-grade triethylamine and acetonitrile were obtained from Thermo Fisher Scientific (Waltham, MA, USA). The purified water used in this study was prepared using a Mille-Q system (EMD Millipore, Billerica, MA, USA). All other reagents were of analytical grade and were purchased from Sinopharm Chemical Reagent Co., Ltd.
5
Evaluation of Cyclosporin Formulations
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Cyclosporin A (CsA) was purchased from Taishang Chemical Pharmacy Company (Taishang, China). Cyclosporin D (CsD) was purchased from Shanghai Tongtian Biotechnology Company (Shanghai, China). Neoral® (Ciclosporin soft gelatin capsules) was purchased from local drugstore. Vitamin E polyethylene glycol succinate (TPGS) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Sodium dodecyl sulfate (SDS) were purchased from BASF, Ludwigshafen, Germany. Hydroxypropyl cellulose (HPC) was purchased from Nippon Soda Co., Ltd. (Tokyo, Japan). Simulated intestinal uid (SIF) and simulated gastric uid (SGF) were purchased from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China). Roswell Park Memorial Institute (RPMI) 1640 medium, phosphate buffered saline (PBS) and Hank's Balanced Salt Solution (HBSS) were purchased from HyClone Co., Ltd. (Utah, USA). Fetal bovine serum (FBS) was purchased from gibco Co., Ltd. (New York, USA). PMSF and RIPA buffer were purchased from Thermo Fisher Scienti c Inc. (Illinois, USA). The puri ed water used in this study was prepared using a Mille-Q system (EMD Millipore, Billerica, MA, USA). HPLC-grade acetonitrile and methanol were obtained from Thermo Fisher Scienti c (Waltham, MA, USA). All other reagents were analytical grade.
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