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Cd3 a0452

Manufactured by Agilent Technologies
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Sourced in Canada, Denmark

The CD3 (A0452) is a lab equipment product from Agilent Technologies. It is designed to measure and analyze cellular samples. The core function of this product is to detect and quantify the presence of CD3, a protein found on the surface of T cells. This information can be used in various immunological and cell biology applications.

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Lab products found in correlation

Pannoramic viewer 1, by 3DHISTECH (2 mentions) Cleaved caspase 3 asp175, by Cell Signaling Technology (2 mentions) Axioimager z2 system, by Zeiss (2 mentions) Ki67 vp k451, by Vector Laboratories (2 mentions) Pannoramic scan 250 flash, by 3DHISTECH (2 mentions) Bond max, by Leica (1 mentions) Pd l1 clone e1l3n, by Cell Signaling Technology (1 mentions) Cd8 c8 144b, by Thermo Fisher Scientific (1 mentions) Cd8 clone c8 144b, by Agilent Technologies (1 mentions) Discovery xt, by Roche (1 mentions) Foxp3 clone 236a e7, by Thermo Fisher Scientific (1 mentions) Pd l1 e1l3n, by Cell Signaling Technology (1 mentions) Pd 1 clone nat105, by Abcam (1 mentions) Bcl2 124, by Agilent Technologies (1 mentions) Bcl 6 ln22, by Leica (1 mentions) Ki 67 mib 1, by Agilent Technologies (1 mentions)

Spelling variants of this product

A0452 (81 citations) Anti-CD3 (A0452; (3 citations) Anti-CD3 (clone A0452; (2 citations)

5 protocols using cd3 a0452

1

Automated Multiplex IHC Analysis

Cited 1 time
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Immunohistochemistry for TUNEL and Ki-67 was performed as previously described (19 (link)). For the PROSPECT samples 4μm- thick tissue sections were stained using an automated staining system (Leica Bond Max, Leica Microsystems, Vista, CA, USA), according to standard protocols. The38 Aperio Image Analysis Toolbox (Aperio, Leica Microsystems) was used for digital analysis of images obtained from scanned slides. PDL1 clone E1L3N from Cell Signaling Technologies, CD3 A0452 from Dako and CD8 C8/144B from Thermo Scientific were used.
Koyama S., Akbay E.A., Li Y.Y., Aref A.R., Skoulidis F., Herter-Sprie G.S., Buczkowski K.A., Liu Y., Awad M.M., Denning W.L., Diao L., Wang J., Parra-Cuentas E.R., Wistuba I.I., Soucheray M., Thai T.C., Asahina H., Kitajima S., Altabef A., Cavanaugh J.D., Rhee K., Gao P., Zhang H., Fecci P.E., Shimamura T., Hellmann M.D., Heymach J.V., Hodi F.S., Freeman G.J., Barbie D.A., Dranoff G., Hammerman P.S, & Wong K.K. (2016). STK11/LKB1 deficiency promotes neutrophil recruitment and proinflammatory cytokine production to suppress T cell activity in the lung tumor microenvironment. Cancer research, 76(5), 999-1008.
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2

Histological Analysis of Organ Samples

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Organ samples were fixed in fresh 4% paraformaldehyde at 4 °C overnight and further subjected to routine histological procedures for embedding in paraffin. 5-μm sections of the samples from at least three different animals per group were placed on microscopic slides next to one another to enable cross-comparison within a slide after H&E staining or IHC staining with the antibodies indicated. Antibodies used were GFP (D5.1) XP, PTEN (D4.3), phospho (p)-AKT (S473) (D9E), p-ribosomal protein S6 (S235/236) (D57.2.2E), Cleaved Caspase-3 (Asp175) (all: Cell Signaling Technologies, Danvers, MA), Ki67 (VP-K451, Vector Laboratories, Burlingame, CA), and CD3 (A0452) (DAKO, Glostrup, Denmark). Stained slides were scanned with a Pannoramic Scan 250 Flash or MIDI system and images acquired using Pannoramic Viewer 1.15.2 (both: 3DHistech, Budapest, Hungary). Additional images were taken on a Zeiss Axio Imager.Z2 system.
Miething C., Scuoppo C., Bosbach B., Appelmann I., Nakitandwe J., Ma J., Wu G., Lintault L., Auer M., Premsrirut P.K., Teruya-Feldstein J., Hicks J., Benveniste H., Speicher M.R., Downing J.R, & Lowe S.W. (2014). PTEN action in leukemia dictated by the tissue microenvironment. Nature, 510(7505), 402-406.
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3

Immunohistochemistry for Immune Biomarkers

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Immunohistochemistry for all antibodies was performed using 3 μm thick slides and standard protocols on the automated IHC staining system Discovery XT (Roche/Ventana, Tucson, Arizona, USA). The following antibodies were used: CD3 (A0452, dilution 1:500, DAKO, Glostrup, Denmark), CD8 (clone C8/144B, dilution 1:100, DAKO, Glostrup, Denmark), PD-1 (clone NAT105; dilution 1:50; Abcam, Cambridge, United Kingdom), PD-L1 (E1L3N; dilution 1:200; Cell Signaling, Boston, U.S.A.), FOXP3 (clone 236A/E7; dilution 1:100; eBioscience, San Diego, U.S.A.) Slides were counterstained with hematoxylin and mounted.
Harter P.N., Bernatz S., Scholz A., Zeiner P.S., Zinke J., Kiyose M., Blasel S., Beschorner R., Senft C., Bender B., Ronellenfitsch M.W., Wikman H., Glatzel M., Meinhardt M., Juratli T.A., Steinbach J.P., Plate K.H., Wischhusen J., Weide B, & Mittelbronn M. (2015). Distribution and prognostic relevance of tumor-infiltrating lymphocytes (TILs) and PD-1/PD-L1 immune checkpoints in human brain metastases. Oncotarget, 6(38), 40836-40849.
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4

Immunohistochemical Analysis of Lymphoid Lesions

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Two pathologists (J. Han and J. Sim) reviewed the slides of selected cases. Primary malignant lymphomas and other lymphoproliferative lesions were excluded. IHC staining with CD3 (A0452, Dako, Glostrup, Denmark), CD20 (L26, Leica, Wetzlar, Germany), and Ki-67 (MIB-1, Dako) was performed in all cases. BCL2 (124, Dako), BCL6 (LN22, Novocastra, Newcastle upon Tyne, UK), and kappa and lambda light chain (Dako) IHC staining was performed in only one patient who showed a diffuse pattern (case 6). Additionally, the IgG4:IgG ratio was assessed using IgG (Dako) and IgG4 (MRQ-44, Cell Marque, Rocklin, CA, USA) antibodies in only three cases which showed plasma cell infiltration (cases 1, 2, and 6). Special staining was performed to confirm the presence of microorganisms. Molecular tests such as IgH gene rearrangement test were not performed in any case.
Sim J., Koh H.H., Choi S., Chu J., Kim T.S., Kim H, & Han J. (2018). Pulmonary Nodular Lymphoid Hyperplasia with Mass-Formation: Clinicopathologic Characteristics of Nine Cases and Review of the Literature. Journal of Pathology and Translational Medicine, 52(4), 211-218.
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5

Histological Analysis of Organ Samples

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Organ samples were fixed in fresh 4% paraformaldehyde at 4 °C overnight and further subjected to routine histological procedures for embedding in paraffin. 5-μm sections of the samples from at least three different animals per group were placed on microscopic slides next to one another to enable cross-comparison within a slide after H&E staining or IHC staining with the antibodies indicated. Antibodies used were GFP (D5.1) XP, PTEN (D4.3), phospho (p)-AKT (S473) (D9E), p-ribosomal protein S6 (S235/236) (D57.2.2E), Cleaved Caspase-3 (Asp175) (all: Cell Signaling Technologies, Danvers, MA), Ki67 (VP-K451, Vector Laboratories, Burlingame, CA), and CD3 (A0452) (DAKO, Glostrup, Denmark). Stained slides were scanned with a Pannoramic Scan 250 Flash or MIDI system and images acquired using Pannoramic Viewer 1.15.2 (both: 3DHistech, Budapest, Hungary). Additional images were taken on a Zeiss Axio Imager.Z2 system.
Miething C., Scuoppo C., Bosbach B., Appelmann I., Nakitandwe J., Ma J., Wu G., Lintault L., Auer M., Premsrirut P.K., Teruya-Feldstein J., Hicks J., Benveniste H., Speicher M.R., Downing J.R, & Lowe S.W. (2014). PTEN action in leukemia dictated by the tissue microenvironment. Nature, 510(7505), 402-406.
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