Potato dextrose broth (pdb)

Manufactured by Scharlab

Sourced in Spain

PDB is a laboratory equipment designed for the preparation and storage of phosphate buffer solutions. It provides a reliable and consistent environment for various scientific applications that require a stable phosphate-based buffer system.

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Lab products found in correlation

6 protocols using potato dextrose broth (pdb)

Antimicrobial Biopolymer Barrier Coatings

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Pea starch (S) was purchased from Roquette Laisa España S.A. (Benifaió, Valencia, Spain), poly (vinyl alcohol) (PVA) (M_w: 89,000–98,000, degree of hydrolysis >99%, and viscosity at 4% H₂O, 20 °C is 11.6–15.4 cP) was obtained from Sigma Aldrich Química S.L. (Madrid, Spain) and glycerol and magnesium nitrate-6-hydrate were provided by Panreac Química S.A. (Castellar de Vallès, Barcelona, Spain).
Different natural antimicrobial substances used were: echinacea (E) and horsetail extract (HS) extract from Soria Natural S.A. (Lérida, Spain), liquid smoke (LS), provided by G. Mariani & C. S.p.a. (Cellatica, Italy), neem oil (NO) purchased from Magnolia Holland Ibérica S.A. (Vilassar de Mar, Barcelona, Spain) and oregano essential oil (OEO) from Herbes del Molí (Benimarfull, Alicante, Spain).
Stock culture of Escherichia colli (CECT 515), Listeria innocua (CECT 910), and Asperguillus niger (CECT 20156) supplied by Colección Española de Cultivos Tipos (CECT, Burjassot, Spain) were kept frozen (−25 °C) respectively in Tryptone Soy Broth (TSB, Scharlab, Barcelona, Spain) for bacteria and Potato Dextrose Broth (Scharlab, Barcelona, Spain) for fungus, supplemented with 30% glycerol. Penicillium expansum was provided from the culture collection of Department of Biotechnology (Universitat Politècnica de València, Valencia, Spain).

Cano A., Cháfer M., Chiralt A, & González-Martínez C. (2015). Physical and Antimicrobial Properties of Starch-PVA Blend Films as Affected by the Incorporation of Natural Antimicrobial Agents. Foods, 5(1), 3.

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Microbiological Culture Media Assays

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Microbiological culture media used in this study consisted of potato dextrose broth (PDB) and buffered peptone water (BPW) (purchased from Scharlab (Barcelona, Spain)); tryptic soy broth (TSB) and tryptic soy agar (TSA) (from Labkem (Barcelona, Spain)); potato dextrose agar (PDA) (from Condalab (Madrid, Spain)); reinforced clostridial medium (RCM) (from Thermo Scientific (Madrid, Spain)). Antibiotics, such as kanamycin, gentamycin, erythromycin, ketoconazole, and triphenyl tetrazolium-chloride (TTC) (from Sigma-Aldrich (Madrid, Spain)), were also used on these assays.

Gea-Botella S., Moreno-Chamba B., de la Casa L., Salazar-Bermeo J., Martí N., Martínez-Madrid M.C., Valero M, & Saura D. (2021). Carotenoids from Persimmon (Diospyros kaki Thunb.) Byproducts Exert Photoprotective, Antioxidative and Microbial Anti-Adhesive Effects on HaCaT. Pharmaceutics, 13(11), 1898.

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Electrochemical Detection of Heavy Metals

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Tris hydrochloride (Tris-HCl), potassium chloride (KCl), hydrochloric acid (HCl), low molecular weight chitosan (CS, MW = 50–190 kDa), potassium dichromate (K₂Cr₂O₇), lead (II) nitrate Pb(NO₃)₂, zinc sulfate heptahydrate (ZnSO₄·7H₂O), potassium ferricyanide (K₄Fe(CN)₆), ferric chloride (FeCl₃), tetrachloroauric(III) acid trihydrate, and sodium citrate were obtained from Sigma-Aldrich, Merck KGaA, Darmstadt, Germany. The multi-walled carbon nanotubes (MWCNT) were purchased from Carbon GmbH, Bayreuth, Germany. The electrochemical measurements for chromium and zinc were carried out in a solution containing 0.1 M HCl and 0.1 M KCl, and those for lead in a 0.1 M Tris-HCl buffer pH 5, respectively. All solutions used in the experiments were prepared using ultrapure water (18.25 MΩ·cm) obtained with a Milli-Q^® Integral 5 system, Merck KGaA, Darmstadt, Germany.
Analytical grade HNO₃ 65%, HCl 37%, and HF 50% for the acid digestion of soil samples were obtained from Thermo Fisher Scientific, Waltham, MA, USA. Standard solutions for chromium, lead, and zinc were obtained from Merck, KGaA, Darmstadt, Germany. NaCl, as well as dehydrated culture media, namely PDA, TSA, PDB, TSB, Luria–Bertani, and Sabouraud, were obtained from Scharlau, Sentmenat, Spain.

Firincă C., Zamfir L.G., Constantin M., Răut I., Capră L., Popa D., Jinga M.L., Baroi A.M., Fierăscu R.C., Corneli N.O., Postolache C., Doni M., Gurban A.M., Jecu L, & Șesan T.E. (2023). Microbial Removal of Heavy Metals from Contaminated Environments Using Metal-Resistant Indigenous Strains. Journal of Xenobiotics, 14(1), 51-78.

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Mycelial Growth and Bioactive Metabolite Production

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From a newly prepared stock culture (about 7 days incubation at 30 °C), actively growing mycelia (1 piece of 5 mm diameter) were grown for 10 days in absence of light on agar solid medium with malt extract. The medium composition was (g/L) 30, malt extract (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany); 3, peptone (Scharlau, Scharlab S.L., Spain); 20, agar (Scharlau, Scharlab S.L. Spain); pH = 5.6 ± 0.2 at 25 °C. Pieces of fungal mycelium were inoculated in liquid potato dextrose broth (PDB, Scharlau, Scharlab S.L., Spain), which had the following composition (g/L): 4, potato peptone; 20, glucose; pH = 5.6 ± 0.2 at 25 °C. The flasks were incubated for 7 days, in static conditions and in darkness. The mycelium from the culture broth was washed 3–4 times with sterile water to remove the remaining components of the culture medium. After this, 10 g of wet mycelium was suspended in 100 mL of sterile water and incubated under agitation at 150 rpm, 30 °C, for 3 days in darkness.

Constantin M., Răut I., Suica-Bunghez R., Firinca C., Radu N., Gurban A.M., Preda S., Alexandrescu E., Doni M, & Jecu L. (2023). Ganoderma lucidum-Mediated Green Synthesis of Silver Nanoparticles with Antimicrobial Activity. Materials, 16(12), 4261.

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Purification of Antifungal Protein PgAFP

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P. chrysogenum CECT 20922 was grown in potato dextrose broth (PDB, Scharlab, Barcelona, Spain) pH 4.5, at 25 C for 21 days without shaking. Mycelia were removed, the culture medium was filtered to obtain cell-free medium and PgAFP was obtained by Fast Protein Liquid Chromatography as previously described (Acosta et al., 2009; (link)Rodríguez-Martín et al., 2010) (link). Briefly, the cell-free medium was separated by chromatography on cationic and gel filtration columns, the sub-fractions containing the highest absorbance peaks were tested against reference sensitive moulds, and the extracts containing purified PgAFP were pooled from several batches. The protein concentration was quantified by the Lowry method (Lowry et al., 1951) and the stock solution was diluted in the elution buffer to the desired active concentration range (1.17e75 mg/mL) for the various assays.

Delgado J., Owens R.A., Doyle S., Núñez F, & Asensio M.A. (2017). Quantitative proteomics reveals new insights into calcium-mediated resistance mechanisms in Aspergillus flavus against the antifungal protein PgAFP in cheese. Food microbiology, 66.

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Purification of Antifungal Protein PgAFP

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P. chrysogenum was grown in potato dextrose broth (PDB; Scharlab, Barcelona, Spain) pH 4.5, at 25 °C for 21 days. Mycelium was removed and the culture medium was filtered through a nitrocellulose 0.22-μm pore size (Sartorius, Goettingen, Germany) to obtain cell-free medium. The cellfree medium was applied to an ÄKTA FPLC with a cationic exchange column HiTrap SP HP (Amersham Biosciences, Uppsala, Sweden), equipped with a UV detector at 214 nm. The resulting fraction containing PgAFP was then chromatographed on a HiLoad 26/60 Superdex 75 column for FPLC (Amersham Biosciences, Uppsala, Sweden) as previously described (Acosta et al. 2009) (link) to obtain an extract of the purified protein. The extract containing the purified PgAFP from several batches were pooled in a stock solution. The amount of protein in the stock solution was quantified by the Lowry method (Lowry et al. 1951) . For the various assays, the PgAFP stock solution was diluted to the desired active concentration range according to the aim of the test.

Delgado J., Owens R.A., Doyle S., Asensio M.A, & Núñez F. (2015). Impact of the antifungal protein PgAFP from Penicillium chrysogenum on the protein profile in Aspergillus flavus. Applied microbiology and biotechnology, 99(20).

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