Traf2 f 2

Antibodies reactive to β-actin (clone: AC-15, catalog number: A5441; Sigma-Aldrich), the DYKDDDDK tag (FLAG) (clone: 1E6, code number: 012-22384; Fujifilm Wako Pure Chemical Corporation), RIPK1 (clone: 38/RIP, material number: 610458; BD Biosciences, San Jose, CA, USA), TNF-R1 (H-5, catalog number: sc-8436; Santa Cruz Biotechnology, Dallas, TX, USA), TNF-R1 (C25C1, catalog number: #3736; Cell Signaling Technology, Danvers, MA, USA), TRADD (clone: 37/TRADD, material number: 610572; BD Biosciences), and TRAF2 (F-2, catalog number: sc-136999; Santa Cruz Biotechnology) were used as primary antibodies for Western blotting. A peroxidase-conjugated goat anti-mouse IgG (H + L) antibody (code number: 115-035-146) and peroxidase-conjugated goat anti-rabbit IgG (H + L) antibody (code number: 111-035-144) were obtained from Jackson ImmunoResearch Laboratories (West Groove, PA, USA), which were used as secondary antibodies for Western blotting.

Primary antibodies for γ1-actin (2F3; FUJIFILM Wako Pure Chemical Corporation), FLAG (1E6; FUJIFILM Wako Pure Chemical Corporation), ICAM-1 (15.2; Leinco Technologies, Inc., St. Louis, MO, USA), RelA (F-6; Santa Cruz Biotechnology, Dallas, TX, USA), lamin A/C (E-1; Santa Cruz Biotechnology), GAPDH (6C5; Santa Cruz Biotechnology), IκBα (25/IkBa/MAD-3; BD Biosciences, San Jose, CA, USA), phospho-IκBα (Ser32/36) (5A5; Cell Signaling Technology, Danvers, MA, USA), TNF-R1 (H-5; Santa Cruz Biotechnology), TNF-R1 (C25C1; Cell Signaling Technology), RIPK1 (38/RIP; BD Biosciences), TRADD (37/TRADD; BD Biosciences), and TRAF2 (F-2; Santa Cruz Biotechnology) were used. A peroxidase-conjugated anti-mouse IgG(H + L) antibody (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) and peroxidase-conjugated anti-rabbit IgG(H + L) antibody (Jackson ImmunoResearch Laboratories) were used as secondary antibodies.