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5 protocols using sodium carbonate

1

Synthesis of Iron-Based Nanoparticles

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All reagents were used without purification. Iron(iii) chloride hexahydrate (FeCl3·6H2O, 97%, Sigma-Aldrich), iron(ii) chloride tetrahydrate (FeCl2·4H2O, ≥99%, Sigma-Aldrich), manganese(ii) chloride tetrahydrate (MnCl2·4H2O, ≥98%, Daejung), hydrochloric acid (HCl, 35–37%, Samchun), ammonia solution (NH4OH, 28–30%, Samchun), polyacrylic acid (PAA, MW: 2000, 63 wt% aqueous solution, Acros Organics), sodium hydroxide (NaOH, 93%, Duksan), sodium citrate dihydrate (Na3C6H5O7·2H2O, ≥99%, Daejung), citric acid (C6H8O7, 99.5%, Samchun), sodium phosphate dibasic (Na2HPO4, 99%, Samchun), sodium phosphate monobasic dihydrate (NaH2PO4·2H2O, 98–102%, Samchun), sodium carbonate (Na2CO3, 99%, Samchun), sodium bicarbonate (NaHCO3, 99–100.5%, Samchun), fetal bovine serum (FBS, Merck Millipore), Dulbecco's modified eagle medium (DMEM, Welgene), penicillin–streptomycin (Pen–Strep, Gibco), saline (0.9% NaCl, JW Life Science), cell counting kit-8 (CCK-8, Sigma-Aldrich), and phosphate buffered saline (PBS, pH = 7.4, 10 mM, Sigma-Aldrich) were used.
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2

Antioxidant Capacity Assay Protocol

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DPPH, gallic acid, ABTS, Folin-Ciocalteu’s phenol reagent, tannic acid, and butylated hydroxyanisole (BHA) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Sodium phosphate monobasic monohydrate, sodium phosphate dibasic heptahydrate, trichloroacetic acid, potassium sulfate, and sodium carbonate were purchased from Samchun Chemical Co. (Pyeongtaek, Korea). Potassium ferricyanide and ferric chloride were purchased from Daejung Chemicals (Siheung, Korea).
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Stimuli-Responsive Polymeric Formulations

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Sodium alginate was obtained at Wako Pure Chemical Industries (11 kDa, Osaka, Japan). Folic acid, tetrabutylammonium hydroxide solution (TBAOH), tetrabutylammonium fluoride hydrate (TBAF), 1,1′-carbonyldiimidazole (CDI), soybean oil, 5-aminolevulinic acid hydrochloride (5ALA, 98%), sorbitan oleate (Span 80), and Polysorbate 80 (Tween 80) were supplied from Sigma-Aldrich (St Louis, MO, USA). Hydrogen peroxide (H2O2, 30.0–35.5%), dimethyl sulfoxide (DMSO, 99.8%), sodium carbonate (Na2CO3), and hydrochloric acid (HCl, 35.0–37.0%) were purchased from Samchun (Gyeonggi-do, Korea). 2,4,6-trinitrobenzene sulfonic acid (TNBSA, 5% w/v) solution, and bicinchoninic acid (BCA) assay kit were obtained from Thermo Fisher (Waltham, MA, USA). The MCF-7 (breast adenocarcinoma), A549 (lung carcinoma), and SKOV-3 (ovarian adenocarcinoma) cell lines were procured from Korea Cell Line Bank (Seoul, Korea). The human fibroblast (HFB) cell line was offered by Seoul National University Hospital (Bundang, Gyeonggi-do, Korea). RIPA lysis buffer was purchased from Biosesang (Gyeonggi-do, Korea) and Cell Counting Kit-8 (CCK-8) was acquired by Dojindo Molecular Technologies, Inc. (Kumamoto, Japan).
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Plant-Derived Natural Compound Extraction

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Folin-Ciocalteu’s phenol reagent, vanillin, saponin from quillaja bark, and (+)-catechin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Gallic acid was purchased from Tokyo Chemical Industry (Tokyo, Japan) and sodium carbonate was purchased from Samchun Chemical (Pyeongtaek, South Korea). Tamiflu were obtained from Roche (Seoul, Korea). B. juncea (seed), F. suspensa, (fruit) and I. britannica (seed) were obtained from Kyungdong-Market in Seoul, Korea. B. juncea, F. suspensa, and I. britannica were authenticated by Professor Hyun-Dong Paik at the Laboratory of Biotechnology (Konkuk University, Seoul, Korea) and stored as voucher specimen KU-H13, KU-H22 and KU-H26, respectively.
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5

Preparation of Europium-Doped Polymer Nanoparticles

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Styrene (St) was supplied by Junsei Chemicals (Tokyo, Japan). Europium chloride hexahydrate, trioctylphosphine (P (Oct)3), 2,2’-Azobis 2-methylpropionamidine dihydrochloride and dialysis tubing cellulose membrane (Mw cut-off = 14,400) membrane were purchased from Sigma-Aldrich. Methacrylic acid (MAA) was supplied by Yakuri Pure Chemicals Co., Ltd. (Kyoto, Japan). Potassium persulfate (PPS), ethanol (EtOH), sodium hydroxide (NaOH), hydrochloric acid (HCl) and sodium carbonate (NaCO3) were supplied by Samchun Chemicals (Kyungkido, Republic of Korea)
4,4,4-Trifluoro-1-(2-thienyl)-1,3-butanedione (TFTB) was supplied by Tokyo Chemical Industry (Tokyo, Japan). Polyvinylpyrrolidone (PVP, Mw = 30,000) was purchased from Kanto Chemical (Tokyo, Japan). All of the chemicals were analytical grade and were used as received without further purification when it was not specially mentioned. Water was purified using the ELGA Flex 3 water purification system (VWR Co. Ltd., PA, USA).
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