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Cd45 kromeorange conjugated clone j33

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The CD45 KromeOrange-conjugated (clone J33) is a flow cytometry reagent used for the detection and identification of cells expressing the CD45 antigen. It is a monoclonal antibody conjugated with the KromeOrange fluorescent dye.

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Lab products found in correlation

Navios ex, by Beckman Coulter (2 mentions) Navios ex software v2, by Beckman Coulter (2 mentions) Cd31 pacificblue conjugated clone 5.6e, by Beckman Coulter (2 mentions) Cd73 pe conjugated clone ad 2, by Beckman Coulter (2 mentions) Cd90 fitc conjugated clone f15 42 1 5, by Beckman Coulter (2 mentions) Cd105 pc7 conjugated clone tea3 17.1.1, by Beckman Coulter (2 mentions) Navios ex software, by Beckman Coulter (2 mentions) Perfect count microspheres, by Cytognos (2 mentions) 7 aminoactinomycin d, by Beckman Coulter (2 mentions)

2 protocols using cd45 kromeorange conjugated clone j33

1

Characterization of Mesenchymal Stem Cells by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were enumerated using Perfect-Count Microspheres™ (Cytognos, Salamanca, Spain) microbeads in a Navios EX flow cytometer (Beckman Coulter, Pasadena, CA, USA). Viability (%) was determined using the 7-Amino-Actinomycin D (7-AAD, Beckman Coulter, Pasadena, CA, USA) exclusion method. Data were analyzed with Navios EX Software v2.0 (Beckman Coulter) software. In accordance with de International Society on Cell and Gene Therapy (ISCT) criteria [3 (link)], identity of MSC was evaluated by the expression of surface markers CD31 PacificBlue-conjugated (clone 5.6E; Beckman Coulter), CD45 KromeOrange-conjugated (clone J33; Beckman Coulter), CD73 PE-conjugated (clone AD-2; Beckman Coulter), CD90 FITC-conjugated (clone F15-42–1-5, Beckman Coulter), CD105 PC7-conjugated (clone TEA3/17.1.1; Beckman Coulter), and HLA-DR APC-conjugated (clone Immu-357; Beckman Coulter) in a Navios EX Device. Cells were stained for 15 min at room temperature, washed and resuspendend with DPBS. Acquisition was done using Navios EX, and raw data were analyzed with Navios EX Software (version 2.0, Beckman Coulter).
Torrents S., del Moral A.E., Codinach M., Rodríguez L., Querol S, & Vives J. (2023). Optimized reagents for immunopotency assays on mesenchymal stromal cells for clinical use. Immunologic Research, 71(5), 725-734.
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2

Characterization of Mesenchymal Stem Cells by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were enumerated using Perfect-Count Microspheres™ (Cytognos, Salamanca, Spain) microbeads in a Navios EX flow cytometer (Beckman Coulter, Pasadena, CA, USA). Viability (%) was determined using the 7-Amino-Actinomycin D (7-AAD, Beckman Coulter, Pasadena, CA, USA) exclusion method. Data were analyzed with Navios EX Software v2.0 (Beckman Coulter) software. In accordance with de International Society on Cell and Gene Therapy (ISCT) criteria [3 (link)], identity of MSC was evaluated by the expression of surface markers CD31 PacificBlue-conjugated (clone 5.6E; Beckman Coulter), CD45 KromeOrange-conjugated (clone J33; Beckman Coulter), CD73 PE-conjugated (clone AD-2; Beckman Coulter), CD90 FITC-conjugated (clone F15-42–1-5, Beckman Coulter), CD105 PC7-conjugated (clone TEA3/17.1.1; Beckman Coulter), and HLA-DR APC-conjugated (clone Immu-357; Beckman Coulter) in a Navios EX Device. Cells were stained for 15 min at room temperature, washed and resuspendend with DPBS. Acquisition was done using Navios EX, and raw data were analyzed with Navios EX Software (version 2.0, Beckman Coulter).
Torrents S., del Moral A.E., Codinach M., Rodríguez L., Querol S, & Vives J. (2023). Optimized reagents for immunopotency assays on mesenchymal stromal cells for clinical use. Immunologic Research, 71(5), 725-734.
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